Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine
Arginine (Arg) as an important substrate for protein metabolism and endocrine influences on fish health directly. To evaluate the effect of dietary Arg on these endocrine and immunity of juvenile orange-spotted grouper (Epinephelus coioides), an experiment adding Arg with 0.00, 0.30, 0.60, 0.90, 1.2...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-05-01
|
| Series: | Aquaculture Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513417301370 |
| _version_ | 1818189694952800256 |
|---|---|
| author | Fenglu Han Shuyan Chi Beiping Tan Xiaohui Dong Qihui Yang Hongyu Liu Shuang Zhang Di Liu Yuanfa He |
| author_facet | Fenglu Han Shuyan Chi Beiping Tan Xiaohui Dong Qihui Yang Hongyu Liu Shuang Zhang Di Liu Yuanfa He |
| author_sort | Fenglu Han |
| collection | DOAJ |
| description | Arginine (Arg) as an important substrate for protein metabolism and endocrine influences on fish health directly. To evaluate the effect of dietary Arg on these endocrine and immunity of juvenile orange-spotted grouper (Epinephelus coioides), an experiment adding Arg with 0.00, 0.30, 0.60, 0.90, 1.20, 1.50, and 1.80% in seven diets was conducted. Dietary Arg levels reached 2.13 (Diet 1), 2.42 (Diet 2), 2.71 (Diet 3), 2.95 (Diet 4), 3.20 (Diet 5), 3.48 (Diet 6) and 3.74% (Diet 7), respectively. Each treatment was randomly assigned to triplicate groups of 30 fish (7.52 ± 0.02 g) each tank. The present results indicated that different Arg levels of diets were no significant effects on the fish survival rate of each groups (P > 0.05) after 8 weeks feeding experiment. The highest weight gain and specific growth rate were found in Diet 4, and significantly higher than the other groups (P < 0.05). Hepatic arginase activities were significantly higher in fish fed Diet 6 and Diet 7 compared to the activities of the fish fed first three Diets (P < 0.05). Hepatic ornithine decarboxylase activities of fish fed Diet 1 and Diet 2 were significantly decreased compared to the fish fed Diet 4–Diet 7 (P < 0.05). With ascending dietary Arg, the serum Insulin (INS) and insulin-like growth factor I (IGF-I) levels significantly increased. Gene expression of tissue toll-like receptors 22 in the three tissues were found first up-regulated and then down-regulated with the Arg increasing. Gene expression of major histocompatibility complex II in liver and kidney significantly increased in fish fed Diet 4 and Diet 6, and decreased thereafter. However, gene expression of hepcidin decreased with dietary Arg increased. The present study showed alterations in serum INS and IGF-I levels in response to Arg-induced growth activation. Additionally, the results indicate that Arg has beneficial effects on regulating mRNA expression of immune-associated genes and improving humoral and cellular immunity of fish. Keywords: Epinephelus coioides, Arginine, Growth performance, Metabolism, Immunity |
| first_indexed | 2024-12-11T23:46:54Z |
| format | Article |
| id | doaj.art-557790ef0e5745f3976290dea43ddaa3 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-11T23:46:54Z |
| publishDate | 2018-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-557790ef0e5745f3976290dea43ddaa32022-12-22T00:45:36ZengElsevierAquaculture Reports2352-51342018-05-0110816Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginineFenglu Han0Shuyan Chi1Beiping Tan2Xiaohui Dong3Qihui Yang4Hongyu Liu5Shuang Zhang6Di Liu7Yuanfa He8Laboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCorresponding author at: Laboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.; Laboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaLaboratory of Aquaculture Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaArginine (Arg) as an important substrate for protein metabolism and endocrine influences on fish health directly. To evaluate the effect of dietary Arg on these endocrine and immunity of juvenile orange-spotted grouper (Epinephelus coioides), an experiment adding Arg with 0.00, 0.30, 0.60, 0.90, 1.20, 1.50, and 1.80% in seven diets was conducted. Dietary Arg levels reached 2.13 (Diet 1), 2.42 (Diet 2), 2.71 (Diet 3), 2.95 (Diet 4), 3.20 (Diet 5), 3.48 (Diet 6) and 3.74% (Diet 7), respectively. Each treatment was randomly assigned to triplicate groups of 30 fish (7.52 ± 0.02 g) each tank. The present results indicated that different Arg levels of diets were no significant effects on the fish survival rate of each groups (P > 0.05) after 8 weeks feeding experiment. The highest weight gain and specific growth rate were found in Diet 4, and significantly higher than the other groups (P < 0.05). Hepatic arginase activities were significantly higher in fish fed Diet 6 and Diet 7 compared to the activities of the fish fed first three Diets (P < 0.05). Hepatic ornithine decarboxylase activities of fish fed Diet 1 and Diet 2 were significantly decreased compared to the fish fed Diet 4–Diet 7 (P < 0.05). With ascending dietary Arg, the serum Insulin (INS) and insulin-like growth factor I (IGF-I) levels significantly increased. Gene expression of tissue toll-like receptors 22 in the three tissues were found first up-regulated and then down-regulated with the Arg increasing. Gene expression of major histocompatibility complex II in liver and kidney significantly increased in fish fed Diet 4 and Diet 6, and decreased thereafter. However, gene expression of hepcidin decreased with dietary Arg increased. The present study showed alterations in serum INS and IGF-I levels in response to Arg-induced growth activation. Additionally, the results indicate that Arg has beneficial effects on regulating mRNA expression of immune-associated genes and improving humoral and cellular immunity of fish. Keywords: Epinephelus coioides, Arginine, Growth performance, Metabolism, Immunityhttp://www.sciencedirect.com/science/article/pii/S2352513417301370 |
| spellingShingle | Fenglu Han Shuyan Chi Beiping Tan Xiaohui Dong Qihui Yang Hongyu Liu Shuang Zhang Di Liu Yuanfa He Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine Aquaculture Reports |
| title | Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine |
| title_full | Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine |
| title_fullStr | Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine |
| title_full_unstemmed | Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine |
| title_short | Metabolic and immune effects of orange-spotted grouper, Epinephelus coioides induced by dietary arginine |
| title_sort | metabolic and immune effects of orange spotted grouper epinephelus coioides induced by dietary arginine |
| url | http://www.sciencedirect.com/science/article/pii/S2352513417301370 |
| work_keys_str_mv | AT fengluhan metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT shuyanchi metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT beipingtan metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT xiaohuidong metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT qihuiyang metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT hongyuliu metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT shuangzhang metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT diliu metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine AT yuanfahe metabolicandimmuneeffectsoforangespottedgrouperepinepheluscoioidesinducedbydietaryarginine |