Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
Regeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic a...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642300296X |
| _version_ | 1797598010950025216 |
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| author | Xuechen Yin Sen Lin Ying Xiong Peng Zhang Xifan Mei |
| author_facet | Xuechen Yin Sen Lin Ying Xiong Peng Zhang Xifan Mei |
| author_sort | Xuechen Yin |
| collection | DOAJ |
| description | Regeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic acid) nanoparticles (PLGA) obtained by loading celastrol (pCel) for SCI thrapy. Cel, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the generation of pro-inflammatory cytokines. To facilitate its administration, pCel is formulated into microspheres by oil-in-water (O/W) emulsion/solvent evaporation technique. The constructed pCel can induced polarization of macrophages and obviously improved lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-induced mitochondrial dysfunction, and increased neurite length in PC12 cells and primary neurons. In vivo experiments revealed that pCel regulated the phenotypic polarization of macrophages, prevented the release of pro-inflammatory cytokines, promoted myelin regeneration and inhibited scar tissue formation, and further improve motor function. These findings indicated that the neuroprotective effect of this artificial biodegradable nanoplatform is benefit for the therapy of SCI. This research opens an exciting perspective for the application of SCI treatment and supports the clinical significance of pCel. |
| first_indexed | 2024-03-11T03:13:22Z |
| format | Article |
| id | doaj.art-557a0298e8fd4f3d9996966a9c3d2879 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-11T03:13:22Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-557a0298e8fd4f3d9996966a9c3d28792023-11-18T04:29:33ZengElsevierMaterials Today Bio2590-00642023-12-0123100836Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in miceXuechen Yin0Sen Lin1Ying Xiong2Peng Zhang3Xifan Mei4Department of Laboratory Medicine, Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, ChinaDepartment of Orthopedic, Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, ChinaNormandie Université, ENSICAEN, UNICAEN, CNRS, Laboratoire Catalyse et Spectrochimie (LCS), FranceDepartment of Orthopedic, Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, China; Corresponding authorDepartment of Orthopedic, Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, China; Corresponding authorRegeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic acid) nanoparticles (PLGA) obtained by loading celastrol (pCel) for SCI thrapy. Cel, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the generation of pro-inflammatory cytokines. To facilitate its administration, pCel is formulated into microspheres by oil-in-water (O/W) emulsion/solvent evaporation technique. The constructed pCel can induced polarization of macrophages and obviously improved lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-induced mitochondrial dysfunction, and increased neurite length in PC12 cells and primary neurons. In vivo experiments revealed that pCel regulated the phenotypic polarization of macrophages, prevented the release of pro-inflammatory cytokines, promoted myelin regeneration and inhibited scar tissue formation, and further improve motor function. These findings indicated that the neuroprotective effect of this artificial biodegradable nanoplatform is benefit for the therapy of SCI. This research opens an exciting perspective for the application of SCI treatment and supports the clinical significance of pCel.http://www.sciencedirect.com/science/article/pii/S259000642300296XBiomimetic nanoplatformSpinal cord injuryPolarizationImmunoengineeringNeuroinflammation |
| spellingShingle | Xuechen Yin Sen Lin Ying Xiong Peng Zhang Xifan Mei Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice Materials Today Bio Biomimetic nanoplatform Spinal cord injury Polarization Immunoengineering Neuroinflammation |
| title | Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| title_full | Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| title_fullStr | Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| title_full_unstemmed | Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| title_short | Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| title_sort | biomimetic nanoplatform with anti inflammation and neuroprotective effects for repairing spinal cord injury in mice |
| topic | Biomimetic nanoplatform Spinal cord injury Polarization Immunoengineering Neuroinflammation |
| url | http://www.sciencedirect.com/science/article/pii/S259000642300296X |
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