Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation?
The aim of this study was to define the relationship between several environmental, laboratory, and genetic factors, i.e., TLR2 and NLRP3 polymorphisms, and <i>Helicobacter pylori</i> (<i>H. pylori</i>) infection in children, by comparing three different groups of pediatric s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Children |
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| Online Access: | https://www.mdpi.com/2227-9067/9/2/121 |
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| author | Lorena Elena Meliț Cristina Oana Mărginean Maria Oana Săsăran Simona Mocanu Dana Valentina Ghiga Adriana Crișan Claudia Bănescu |
| author_facet | Lorena Elena Meliț Cristina Oana Mărginean Maria Oana Săsăran Simona Mocanu Dana Valentina Ghiga Adriana Crișan Claudia Bănescu |
| author_sort | Lorena Elena Meliț |
| collection | DOAJ |
| description | The aim of this study was to define the relationship between several environmental, laboratory, and genetic factors, i.e., TLR2 and NLRP3 polymorphisms, and <i>Helicobacter pylori</i> (<i>H. pylori</i>) infection in children, by comparing three different groups of pediatric subjects: <i>H. pylori</i>-induced gastritis, non-<i>H. pylori</i> gastritis, and healthy controls. Our final study sample included 269 children, which were divided into three groups according to the histopathological exam: group 1 with 51 children with <i>H. pylori</i>-induced gastritis, group 2 with 103 children with <i>H. pylori</i>-negative gastritis, and group 3 (control group) with 115 children without any histopathological changes. All children underwent a thorough anamnesis, clinical exam, laboratory tests, and upper digestive endoscopy with gastric biopsy for rapid urease test, histopathological exam, and genetic analysis of <i>TLR2</i> rs3804099, <i>TLR2</i> rs3804100, and <i>NLRP3</i> rs10754558 gene polymorphisms. We noticed a significant association between living conditions and the type of gastritis (<i>p</i> < 0.0001). Both rapid urease and serological tests were significantly associated with the presence of <i>H. pylori</i> (<i>p</i> < 0.0001). The CT variant genotype of <i>TLR2</i> rs380499 was significantly associated with neutrophil count (<i>p</i> = 0.0325). We noticed a significant association between the CC variant genotype of <i>NLRP3</i> rs10754558 and leucocytes, neutrophils, eosinophils, as well as ALT (<i>p</i> = 0.0185, <i>p</i> = 0.0379, <i>p</i> = 0.0483, <i>p</i> = 0.0356). Based on these findings, we state that poor living conditions and rural areas represent risk factors for <i>H. pylori</i> infection. The rapid urease test is a reliable diagnostic tool for this infection. CT and TT carriers of <i>TLR2</i> rs3804099, as well as CC carriers of <i>NLRP3</i> rs10754558, might display a more severe degree of systemic inflammation. |
| first_indexed | 2024-03-09T22:19:08Z |
| format | Article |
| id | doaj.art-557a12a7711c40aabe420b11b547d833 |
| institution | Directory Open Access Journal |
| issn | 2227-9067 |
| language | English |
| last_indexed | 2024-03-09T22:19:08Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Children |
| spelling | doaj.art-557a12a7711c40aabe420b11b547d8332023-11-23T19:17:46ZengMDPI AGChildren2227-90672022-01-019212110.3390/children9020121Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation?Lorena Elena Meliț0Cristina Oana Mărginean1Maria Oana Săsăran2Simona Mocanu3Dana Valentina Ghiga4Adriana Crișan5Claudia Bănescu6Department of Pediatrics I, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaDepartment of Pediatrics I, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaDepartment of Pediatrics III, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaDepartment of Pathology, County Emergency Clinical Hospital of Târgu Mureș, Gheorghe Marinescu Street No. 50, 540136 Târgu Mureș, RomaniaDepartment of Scientific Medical Research Methodology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaDepartment of Genetics, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaDepartment of Genetics, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, Gheorghe Marinescu Street No. 38, 540136 Târgu Mureș, RomaniaThe aim of this study was to define the relationship between several environmental, laboratory, and genetic factors, i.e., TLR2 and NLRP3 polymorphisms, and <i>Helicobacter pylori</i> (<i>H. pylori</i>) infection in children, by comparing three different groups of pediatric subjects: <i>H. pylori</i>-induced gastritis, non-<i>H. pylori</i> gastritis, and healthy controls. Our final study sample included 269 children, which were divided into three groups according to the histopathological exam: group 1 with 51 children with <i>H. pylori</i>-induced gastritis, group 2 with 103 children with <i>H. pylori</i>-negative gastritis, and group 3 (control group) with 115 children without any histopathological changes. All children underwent a thorough anamnesis, clinical exam, laboratory tests, and upper digestive endoscopy with gastric biopsy for rapid urease test, histopathological exam, and genetic analysis of <i>TLR2</i> rs3804099, <i>TLR2</i> rs3804100, and <i>NLRP3</i> rs10754558 gene polymorphisms. We noticed a significant association between living conditions and the type of gastritis (<i>p</i> < 0.0001). Both rapid urease and serological tests were significantly associated with the presence of <i>H. pylori</i> (<i>p</i> < 0.0001). The CT variant genotype of <i>TLR2</i> rs380499 was significantly associated with neutrophil count (<i>p</i> = 0.0325). We noticed a significant association between the CC variant genotype of <i>NLRP3</i> rs10754558 and leucocytes, neutrophils, eosinophils, as well as ALT (<i>p</i> = 0.0185, <i>p</i> = 0.0379, <i>p</i> = 0.0483, <i>p</i> = 0.0356). Based on these findings, we state that poor living conditions and rural areas represent risk factors for <i>H. pylori</i> infection. The rapid urease test is a reliable diagnostic tool for this infection. CT and TT carriers of <i>TLR2</i> rs3804099, as well as CC carriers of <i>NLRP3</i> rs10754558, might display a more severe degree of systemic inflammation.https://www.mdpi.com/2227-9067/9/2/121innate immune responsechildrengastritisTLR2 rs3804099 gene polymorphismsTLR2 rs3804100 gene polymorphismsNLRP3 rs10754558 gene polymorphisms |
| spellingShingle | Lorena Elena Meliț Cristina Oana Mărginean Maria Oana Săsăran Simona Mocanu Dana Valentina Ghiga Adriana Crișan Claudia Bănescu Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? Children innate immune response children gastritis TLR2 rs3804099 gene polymorphisms TLR2 rs3804100 gene polymorphisms NLRP3 rs10754558 gene polymorphisms |
| title | Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? |
| title_full | Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? |
| title_fullStr | Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? |
| title_full_unstemmed | Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? |
| title_short | Innate Immune Responses in Pediatric Patients with Gastritis—A Trademark of Infection or Chronic Inflammation? |
| title_sort | innate immune responses in pediatric patients with gastritis a trademark of infection or chronic inflammation |
| topic | innate immune response children gastritis TLR2 rs3804099 gene polymorphisms TLR2 rs3804100 gene polymorphisms NLRP3 rs10754558 gene polymorphisms |
| url | https://www.mdpi.com/2227-9067/9/2/121 |
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