Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand
Members of the <i>Bacillus cereus</i> group are spore-forming Gram-positive bacilli that are commonly associated with diarrheal or emetic food poisoning. They are widespread in nature and frequently present in both raw and processed food products. Here, we genetically characterized 24 &l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/13/3/245 |
| _version_ | 1797242272342867968 |
|---|---|
| author | Phornphan Sornchuer Kritsakorn Saninjuk Sumet Amonyingcharoen Jittiporn Ruangtong Nattaya Thongsepee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj |
| author_facet | Phornphan Sornchuer Kritsakorn Saninjuk Sumet Amonyingcharoen Jittiporn Ruangtong Nattaya Thongsepee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj |
| author_sort | Phornphan Sornchuer |
| collection | DOAJ |
| description | Members of the <i>Bacillus cereus</i> group are spore-forming Gram-positive bacilli that are commonly associated with diarrheal or emetic food poisoning. They are widespread in nature and frequently present in both raw and processed food products. Here, we genetically characterized 24 <i>B. cereus</i> group isolates from foodstuffs. Whole-genome sequencing (WGS) revealed that most of the isolates were closely related to <i>B. cereus sensu stricto</i> (12 isolates), followed by <i>B. pacificus</i> (5 isolates), <i>B. paranthracis</i> (5 isolates), <i>B. tropicus</i> (1 isolate), and “<i>B. bingmayongensis</i>” (1 isolate). The most detected virulence genes were <i>BAS_RS06430</i>, followed by bacillibactin biosynthesis genes (<i>dhbA</i>, <i>dhbB</i>, <i>dhbC</i>, <i>dhbE</i>, and <i>dhbF</i>), genes encoding the three-component non-hemolytic enterotoxin (<i>nheA</i>, <i>nheB</i>, and <i>nheC</i>), a gene encoding an iron-regulated leucine-rich surface protein (<i>ilsA</i>), and a gene encoding a metalloprotease (<i>inhA</i>). Various biofilm-associated genes were found, with high prevalences of <i>tasA</i> and <i>sipW</i> genes (matrix protein-encoding genes); <i>purA</i>, <i>purC</i>, and <i>purL</i> genes (eDNA synthesis genes); <i>lytR</i> and <i>ugd</i> genes (matrix polysaccharide synthesis genes); and <i>abrB</i>, <i>codY</i>, <i>nprR</i>, <i>plcR</i>, <i>sinR</i>, and <i>spo0A</i> genes (biofilm transcription regulator genes). Genes related to fosfomycin and beta-lactam resistance were identified in most of the isolates. We therefore demonstrated that WGS analysis represents a useful tool for rapidly identifying and characterizing <i>B. cereus</i> group strains. Determining the genetic epidemiology, the presence of virulence and antimicrobial resistance genes, and the pathogenic potential of each strain is crucial for improving the risk assessment of foodborne <i>B. cereus</i> group strains. |
| first_indexed | 2024-04-24T18:36:35Z |
| format | Article |
| id | doaj.art-557cdf967aea4b0f8cdb47fcfcc8b0aa |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-04-24T18:36:35Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-557cdf967aea4b0f8cdb47fcfcc8b0aa2024-03-27T13:18:10ZengMDPI AGAntibiotics2079-63822024-03-0113324510.3390/antibiotics13030245Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in ThailandPhornphan Sornchuer0Kritsakorn Saninjuk1Sumet Amonyingcharoen2Jittiporn Ruangtong3Nattaya Thongsepee4Pongsakorn Martviset5Pathanin Chantree6Kant Sangpairoj7Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandPorcinotec Co., Ltd., Nonthaburi 11000, ThailandMedical Life Sciences Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandThammasat University Research Unit in Nutraceuticals and Food Safety, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandDepartment of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, ThailandMembers of the <i>Bacillus cereus</i> group are spore-forming Gram-positive bacilli that are commonly associated with diarrheal or emetic food poisoning. They are widespread in nature and frequently present in both raw and processed food products. Here, we genetically characterized 24 <i>B. cereus</i> group isolates from foodstuffs. Whole-genome sequencing (WGS) revealed that most of the isolates were closely related to <i>B. cereus sensu stricto</i> (12 isolates), followed by <i>B. pacificus</i> (5 isolates), <i>B. paranthracis</i> (5 isolates), <i>B. tropicus</i> (1 isolate), and “<i>B. bingmayongensis</i>” (1 isolate). The most detected virulence genes were <i>BAS_RS06430</i>, followed by bacillibactin biosynthesis genes (<i>dhbA</i>, <i>dhbB</i>, <i>dhbC</i>, <i>dhbE</i>, and <i>dhbF</i>), genes encoding the three-component non-hemolytic enterotoxin (<i>nheA</i>, <i>nheB</i>, and <i>nheC</i>), a gene encoding an iron-regulated leucine-rich surface protein (<i>ilsA</i>), and a gene encoding a metalloprotease (<i>inhA</i>). Various biofilm-associated genes were found, with high prevalences of <i>tasA</i> and <i>sipW</i> genes (matrix protein-encoding genes); <i>purA</i>, <i>purC</i>, and <i>purL</i> genes (eDNA synthesis genes); <i>lytR</i> and <i>ugd</i> genes (matrix polysaccharide synthesis genes); and <i>abrB</i>, <i>codY</i>, <i>nprR</i>, <i>plcR</i>, <i>sinR</i>, and <i>spo0A</i> genes (biofilm transcription regulator genes). Genes related to fosfomycin and beta-lactam resistance were identified in most of the isolates. We therefore demonstrated that WGS analysis represents a useful tool for rapidly identifying and characterizing <i>B. cereus</i> group strains. Determining the genetic epidemiology, the presence of virulence and antimicrobial resistance genes, and the pathogenic potential of each strain is crucial for improving the risk assessment of foodborne <i>B. cereus</i> group strains.https://www.mdpi.com/2079-6382/13/3/245<i>Bacillus cereus</i>foodborne pathogenantimicrobial resistance genevirulence genewhole-genome sequencing |
| spellingShingle | Phornphan Sornchuer Kritsakorn Saninjuk Sumet Amonyingcharoen Jittiporn Ruangtong Nattaya Thongsepee Pongsakorn Martviset Pathanin Chantree Kant Sangpairoj Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand Antibiotics <i>Bacillus cereus</i> foodborne pathogen antimicrobial resistance gene virulence gene whole-genome sequencing |
| title | Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand |
| title_full | Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand |
| title_fullStr | Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand |
| title_full_unstemmed | Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand |
| title_short | Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic <i>B. cereus</i> Group Isolates from Foodstuffs in Thailand |
| title_sort | whole genome sequencing reveals antimicrobial resistance and virulence genes of both pathogenic and non pathogenic i b cereus i group isolates from foodstuffs in thailand |
| topic | <i>Bacillus cereus</i> foodborne pathogen antimicrobial resistance gene virulence gene whole-genome sequencing |
| url | https://www.mdpi.com/2079-6382/13/3/245 |
| work_keys_str_mv | AT phornphansornchuer wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT kritsakornsaninjuk wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT sumetamonyingcharoen wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT jittipornruangtong wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT nattayathongsepee wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT pongsakornmartviset wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT pathaninchantree wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand AT kantsangpairoj wholegenomesequencingrevealsantimicrobialresistanceandvirulencegenesofbothpathogenicandnonpathogenicibcereusigroupisolatesfromfoodstuffsinthailand |