COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA
Aim. Characteristics of clonal composition of Corynebacterium diphtheriae strain populatior in Russia using MLST, as well as evaluation of a possibility of using of this method during execution of monitoring of diphtheria infection causative agent strains. Materials and methods. C. diphtheriae strai...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2016-10-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/87 |
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| author | I. A. Chagina O. Yu. Borisova L. I. Kafarskaya S. S. Afanasiev V. A. Aleshkin Yu. V. Nesvizhsky M. S. Afanasiev A. V. Aleshkin E. V. Yusuf T. I. Moskvina L. I. Ponomareva A. V. Karaulov |
| author_facet | I. A. Chagina O. Yu. Borisova L. I. Kafarskaya S. S. Afanasiev V. A. Aleshkin Yu. V. Nesvizhsky M. S. Afanasiev A. V. Aleshkin E. V. Yusuf T. I. Moskvina L. I. Ponomareva A. V. Karaulov |
| author_sort | I. A. Chagina |
| collection | DOAJ |
| description | Aim. Characteristics of clonal composition of Corynebacterium diphtheriae strain populatior in Russia using MLST, as well as evaluation of a possibility of using of this method during execution of monitoring of diphtheria infection causative agent strains. Materials and methods. C. diphtheriae strains, isolated in Russia in 1957 - 2015 and sent to Gabrichevsky MRIEM reference centre for diphtheria and pertussis, were studied. Genotyping of C. diphtheriae using MLST wa: carried out based on sequencing of «housekeeping» gene fragments. ST identification was carriec out according to PubMLST. Results. C. diphtheriae strains of 36 sequence-types (ST) were identified on the territory of Russia - 27 previously known and 9 novel, detected for the first time. 2 sequence types ST25 and ST8 (22% and 18%) dominated. Inter-relation between phenotype properties (toxigenicity and biovar) and membership of C. diphtheriae strains in certain sequence-types was shown - toxigenic and non-toxigenic C. diphtheriae strains of various biovars were characterized by certain sequence-types. Changes of clonal composition of C. diphtheriae population in dynamics of epidemic process of diphtheria infection were shown. Conclusion. Use of MLST allowed to characterized clonal composition of C. diphtheriae strains’ population in Russia and has shown perspectives of use of this method to characterize population of diphtheria causative agent, detect epidemically significant strains and decipher foci of diphtheria infection. |
| first_indexed | 2024-12-10T19:00:25Z |
| format | Article |
| id | doaj.art-557d3d6b9fe342058cd3326675413ae4 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:00:25Z |
| publishDate | 2016-10-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-557d3d6b9fe342058cd3326675413ae42022-12-22T01:37:01ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132016-10-0105506010.36233/0372-9311-2016-5-50-6087COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIAI. A. Chagina0O. Yu. Borisova1L. I. Kafarskaya2S. S. Afanasiev3V. A. Aleshkin4Yu. V. NesvizhskyM. S. Afanasiev5A. V. Aleshkin6E. V. Yusuf7T. I. Moskvina8L. I. Ponomareva9A. V. Karaulov10Gabrichevsky Moscow Research Institute of Epidemiology and MicrobiologyGabrichevsky Moscow Research Institute of Epidemiology and Microbiology; Pirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityGabrichevsky Moscow Research Institute of Epidemiology and MicrobiologyGabrichevsky Moscow Research Institute of Epidemiology and MicrobiologySechenov First Moscow State Medical UniversityGabrichevsky Moscow Research Institute of Epidemiology and MicrobiologyCentre for Hygiene and Epidemiology in Khanty-Mansi Autonomous District YugraCentre for Hygiene and Epidemiology in Chelyabinsk RegionCentre for Hygiene and Epidemiology in the Udmurt RepublicSechenov First Moscow State Medical UniversityAim. Characteristics of clonal composition of Corynebacterium diphtheriae strain populatior in Russia using MLST, as well as evaluation of a possibility of using of this method during execution of monitoring of diphtheria infection causative agent strains. Materials and methods. C. diphtheriae strains, isolated in Russia in 1957 - 2015 and sent to Gabrichevsky MRIEM reference centre for diphtheria and pertussis, were studied. Genotyping of C. diphtheriae using MLST wa: carried out based on sequencing of «housekeeping» gene fragments. ST identification was carriec out according to PubMLST. Results. C. diphtheriae strains of 36 sequence-types (ST) were identified on the territory of Russia - 27 previously known and 9 novel, detected for the first time. 2 sequence types ST25 and ST8 (22% and 18%) dominated. Inter-relation between phenotype properties (toxigenicity and biovar) and membership of C. diphtheriae strains in certain sequence-types was shown - toxigenic and non-toxigenic C. diphtheriae strains of various biovars were characterized by certain sequence-types. Changes of clonal composition of C. diphtheriae population in dynamics of epidemic process of diphtheria infection were shown. Conclusion. Use of MLST allowed to characterized clonal composition of C. diphtheriae strains’ population in Russia and has shown perspectives of use of this method to characterize population of diphtheria causative agent, detect epidemically significant strains and decipher foci of diphtheria infection.https://microbiol.elpub.ru/jour/article/view/87corynebacterium diphtheriaecorynebacterium diphtheriaemultilocus dna sequencing |
| spellingShingle | I. A. Chagina O. Yu. Borisova L. I. Kafarskaya S. S. Afanasiev V. A. Aleshkin Yu. V. Nesvizhsky M. S. Afanasiev A. V. Aleshkin E. V. Yusuf T. I. Moskvina L. I. Ponomareva A. V. Karaulov COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA Журнал микробиологии, эпидемиологии и иммунобиологии corynebacterium diphtheriae corynebacterium diphtheriae multilocus dna sequencing |
| title | COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA |
| title_full | COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA |
| title_fullStr | COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA |
| title_full_unstemmed | COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA |
| title_short | COMPOSITION OF POPULATION OF DIPHTHERIA CAUSATIVE AGENT STRAINS IN RUSSIA |
| title_sort | composition of population of diphtheria causative agent strains in russia |
| topic | corynebacterium diphtheriae corynebacterium diphtheriae multilocus dna sequencing |
| url | https://microbiol.elpub.ru/jour/article/view/87 |
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