Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm
Intrauterine insemination with donor sperm (IUI-D) requires multiple in vitro manipulations such as sperm selection and cryopreservation during which spermatozoa may be exposed to oxidative stress (OS) and other insults that may produce potential damage including sperm DNA fragmentation (SDF). High...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Journal of Personalized Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4426/13/12/1668 |
| _version_ | 1797380406248472576 |
|---|---|
| author | Alessa Sugihara Usha Punjabi Tiziana Chimienti Ilse Goovaerts Kris Peeters Jason Bouziotis Diane De Neubourg |
| author_facet | Alessa Sugihara Usha Punjabi Tiziana Chimienti Ilse Goovaerts Kris Peeters Jason Bouziotis Diane De Neubourg |
| author_sort | Alessa Sugihara |
| collection | DOAJ |
| description | Intrauterine insemination with donor sperm (IUI-D) requires multiple in vitro manipulations such as sperm selection and cryopreservation during which spermatozoa may be exposed to oxidative stress (OS) and other insults that may produce potential damage including sperm DNA fragmentation (SDF). High levels of SDF, referring to damage or breaks in the genetic material of sperm cells, are linked to an increased risk of reproductive failure. This retrospective, observational study set out to evaluate whether SDF assessment could predict clinical outcome in an IUI-D program, where sperm donors are selected on strict conventional semen parameters. A total of 18 donors and 106 recipients were matched for IUI-D. Out of 429 cycles, 100 (23.3%) resulted in clinical pregnancy. We counted 78 live births (18.2% of cycles), while 20 pregnancies ended in miscarriage (4.7% of cycles), 1 in extra-uterine pregnancy and 1 in stillbirth. Female age significantly influenced clinical pregnancy and miscarriage rates. SDF increased after cryopreservation (26.3 ± 14.5%; <i>p</i> < 0.001) and more so after post-thaw density gradient (34.9 ± 22.1%; <i>p</i> = 0.04) without affecting clinical pregnancy (OR [95% CI] 1.01 [0.99; 1.02]; <i>p</i> = 0.27), live birth (1.00 [0.99; 1.02]; <i>p</i> = 0.72) and miscarriage rates (1.02 [1.00; 1.05]; <i>p</i> = 0.08). The implications of our findings extend to a better selection of sperm donors and a better sperm preparation technique tailored to the donor semen’s properties in order to maximize the chances of a favorable treatment outcome. |
| first_indexed | 2024-03-08T20:36:45Z |
| format | Article |
| id | doaj.art-557df8e1af324628ba39fe6644e20380 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-08T20:36:45Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-557df8e1af324628ba39fe6644e203802023-12-22T14:19:53ZengMDPI AGJournal of Personalized Medicine2075-44262023-11-011312166810.3390/jpm13121668Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor SpermAlessa Sugihara0Usha Punjabi1Tiziana Chimienti2Ilse Goovaerts3Kris Peeters4Jason Bouziotis5Diane De Neubourg6Centre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumCentre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumCentre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumCentre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumCentre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumClinical Trial Center, University Hospital of Antwerp, 2650 Edegem, BelgiumCentre of Reproductive Medicine, University Hospital of Antwerp, 2650 Edegem, BelgiumIntrauterine insemination with donor sperm (IUI-D) requires multiple in vitro manipulations such as sperm selection and cryopreservation during which spermatozoa may be exposed to oxidative stress (OS) and other insults that may produce potential damage including sperm DNA fragmentation (SDF). High levels of SDF, referring to damage or breaks in the genetic material of sperm cells, are linked to an increased risk of reproductive failure. This retrospective, observational study set out to evaluate whether SDF assessment could predict clinical outcome in an IUI-D program, where sperm donors are selected on strict conventional semen parameters. A total of 18 donors and 106 recipients were matched for IUI-D. Out of 429 cycles, 100 (23.3%) resulted in clinical pregnancy. We counted 78 live births (18.2% of cycles), while 20 pregnancies ended in miscarriage (4.7% of cycles), 1 in extra-uterine pregnancy and 1 in stillbirth. Female age significantly influenced clinical pregnancy and miscarriage rates. SDF increased after cryopreservation (26.3 ± 14.5%; <i>p</i> < 0.001) and more so after post-thaw density gradient (34.9 ± 22.1%; <i>p</i> = 0.04) without affecting clinical pregnancy (OR [95% CI] 1.01 [0.99; 1.02]; <i>p</i> = 0.27), live birth (1.00 [0.99; 1.02]; <i>p</i> = 0.72) and miscarriage rates (1.02 [1.00; 1.05]; <i>p</i> = 0.08). The implications of our findings extend to a better selection of sperm donors and a better sperm preparation technique tailored to the donor semen’s properties in order to maximize the chances of a favorable treatment outcome.https://www.mdpi.com/2075-4426/13/12/1668intrauterine inseminationdonor spermheterologous inseminationsperm DNA fragmentationsperm qualitycryopreservation |
| spellingShingle | Alessa Sugihara Usha Punjabi Tiziana Chimienti Ilse Goovaerts Kris Peeters Jason Bouziotis Diane De Neubourg Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm Journal of Personalized Medicine intrauterine insemination donor sperm heterologous insemination sperm DNA fragmentation sperm quality cryopreservation |
| title | Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm |
| title_full | Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm |
| title_fullStr | Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm |
| title_full_unstemmed | Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm |
| title_short | Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm |
| title_sort | sperm dna fragmentation after cryopreservation and sperm selection has no implications for clinical pregnancies and live births after intrauterine insemination with donor sperm |
| topic | intrauterine insemination donor sperm heterologous insemination sperm DNA fragmentation sperm quality cryopreservation |
| url | https://www.mdpi.com/2075-4426/13/12/1668 |
| work_keys_str_mv | AT alessasugihara spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT ushapunjabi spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT tizianachimienti spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT ilsegoovaerts spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT krispeeters spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT jasonbouziotis spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm AT dianedeneubourg spermdnafragmentationaftercryopreservationandspermselectionhasnoimplicationsforclinicalpregnanciesandlivebirthsafterintrauterineinseminationwithdonorsperm |