Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults
IntroductionDrug-sensitive live coccidiosis vaccines have been used to control coccidiosis and renew drug sensitivity in commercial chicken operations. However, only limited species coverage vaccines have been available for commercial turkey producers. This study aimed to assess the effect of an E....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1165317/full |
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| author | Carolina Trujillo-Peralta Juan David Latorre Jianmin Chai Roberto Senas-Cuesta Aaron Forga Makenly Coles Jiangchao Zhao Xochitl Hernandez-Velasco Guillermo Tellez-Isaias John Barta Lisa Bielke Billy Hargis Danielle Graham |
| author_facet | Carolina Trujillo-Peralta Juan David Latorre Jianmin Chai Roberto Senas-Cuesta Aaron Forga Makenly Coles Jiangchao Zhao Xochitl Hernandez-Velasco Guillermo Tellez-Isaias John Barta Lisa Bielke Billy Hargis Danielle Graham |
| author_sort | Carolina Trujillo-Peralta |
| collection | DOAJ |
| description | IntroductionDrug-sensitive live coccidiosis vaccines have been used to control coccidiosis and renew drug sensitivity in commercial chicken operations. However, only limited species coverage vaccines have been available for commercial turkey producers. This study aimed to assess the effect of an E. meleagrimitis vaccine candidate, with and without amprolium intervention, on performance and oocyst shedding. Additionally, the effect of vaccination, amprolium treatment, and E. meleagrimitis challenge on intestinal integrity and microbiome composition was evaluated.MethodsExperimental groups included: (1) NC (non-vaccinated, non-challenged control); (2) PC (non-vaccinated, challenged control); (3) VX + Amprol (E. meleagrimitis candidate vaccine + amprolium); and 4) VX (E. meleagrimitis candidate vaccine). For VX groups, 50% of the direct poults were orally vaccinated at DOH with 50 sporulated E. meleagrimitis oocysts and were comingled with contact or non-vaccinated poults for the duration of the study. From d10-14, VX + Amprol group received amprolium (0.024%) in the drinking water. All groups except NC were orally challenged with 95K E. meleagrimitis sporulated oocysts/mL/poult at d23. At d29, ileal and cecal contents were collected for 16S rRNA gene-based microbiome analysis.Results and DiscussionVX did not affect performance during the pre-challenge period. At d23-29 (post-challenge), VX groups had significantly (P < 0.05) higher BWG than the PC group. Contacts and directs of VX groups in LS had significantly reduced compared to PC. As anticipated, amprolium treatment markedly reduced fecal and litter OPG for the VX + Amprol group compared to the VX group which did not receive amprolium. The ileal and cecal content results showed that the PC group had different bacterial diversity and structure, including alpha and beta diversity, compared to NC. Linear discriminant analysis Effect Size (LEfSe) identified that Lactobacillus salivarius (ASV2) was enriched in PC’s ileal and cecal content. Compared to NC and PC, the vaccinated groups showed no distinct clusters, but there were similarities in the ileal and cecal communities based on Bray-Curtis and Jaccard distances. In conclusion, these results indicate that vaccination with this strain of E. meleagrimitis, with or without amprolium intervention, caused a very mild infection that induced protective immunity and challenge markedly affected both the ileal and cecal microbiome. |
| first_indexed | 2024-03-13T08:06:13Z |
| format | Article |
| id | doaj.art-557f8bb22ad7448bb10495bd194fe874 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T08:06:13Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-557f8bb22ad7448bb10495bd194fe8742023-06-01T04:47:10ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-06-011010.3389/fvets.2023.11653171165317Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poultsCarolina Trujillo-Peralta0Juan David Latorre1Jianmin Chai2Roberto Senas-Cuesta3Aaron Forga4Makenly Coles5Jiangchao Zhao6Xochitl Hernandez-Velasco7Guillermo Tellez-Isaias8John Barta9Lisa Bielke10Billy Hargis11Danielle Graham12Department of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesSchool of Life Science and Engineering, Foshan University, Foshan, ChinaDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, AR, United StatesDepartamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, MexicoDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, CanadaDepartment of Animal Sciences, The Ohio State University, Wooster, OH, United StatesDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesDepartment of Poultry Science, University of Arkansas Division of Agriculture, Fayetteville, AR, United StatesIntroductionDrug-sensitive live coccidiosis vaccines have been used to control coccidiosis and renew drug sensitivity in commercial chicken operations. However, only limited species coverage vaccines have been available for commercial turkey producers. This study aimed to assess the effect of an E. meleagrimitis vaccine candidate, with and without amprolium intervention, on performance and oocyst shedding. Additionally, the effect of vaccination, amprolium treatment, and E. meleagrimitis challenge on intestinal integrity and microbiome composition was evaluated.MethodsExperimental groups included: (1) NC (non-vaccinated, non-challenged control); (2) PC (non-vaccinated, challenged control); (3) VX + Amprol (E. meleagrimitis candidate vaccine + amprolium); and 4) VX (E. meleagrimitis candidate vaccine). For VX groups, 50% of the direct poults were orally vaccinated at DOH with 50 sporulated E. meleagrimitis oocysts and were comingled with contact or non-vaccinated poults for the duration of the study. From d10-14, VX + Amprol group received amprolium (0.024%) in the drinking water. All groups except NC were orally challenged with 95K E. meleagrimitis sporulated oocysts/mL/poult at d23. At d29, ileal and cecal contents were collected for 16S rRNA gene-based microbiome analysis.Results and DiscussionVX did not affect performance during the pre-challenge period. At d23-29 (post-challenge), VX groups had significantly (P < 0.05) higher BWG than the PC group. Contacts and directs of VX groups in LS had significantly reduced compared to PC. As anticipated, amprolium treatment markedly reduced fecal and litter OPG for the VX + Amprol group compared to the VX group which did not receive amprolium. The ileal and cecal content results showed that the PC group had different bacterial diversity and structure, including alpha and beta diversity, compared to NC. Linear discriminant analysis Effect Size (LEfSe) identified that Lactobacillus salivarius (ASV2) was enriched in PC’s ileal and cecal content. Compared to NC and PC, the vaccinated groups showed no distinct clusters, but there were similarities in the ileal and cecal communities based on Bray-Curtis and Jaccard distances. In conclusion, these results indicate that vaccination with this strain of E. meleagrimitis, with or without amprolium intervention, caused a very mild infection that induced protective immunity and challenge markedly affected both the ileal and cecal microbiome. https://www.frontiersin.org/articles/10.3389/fvets.2023.1165317/fullEimeriacoccidiosisvaccinationturkeymicrobiomeamprolium |
| spellingShingle | Carolina Trujillo-Peralta Juan David Latorre Jianmin Chai Roberto Senas-Cuesta Aaron Forga Makenly Coles Jiangchao Zhao Xochitl Hernandez-Velasco Guillermo Tellez-Isaias John Barta Lisa Bielke Billy Hargis Danielle Graham Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults Frontiers in Veterinary Science Eimeria coccidiosis vaccination turkey microbiome amprolium |
| title | Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults |
| title_full | Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults |
| title_fullStr | Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults |
| title_full_unstemmed | Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults |
| title_short | Impact of Eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of Turkey poults |
| title_sort | impact of eimeria meleagrimitis and intermittent amprolium treatment on performance and the gut microbiome composition of turkey poults |
| topic | Eimeria coccidiosis vaccination turkey microbiome amprolium |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1165317/full |
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