Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk.
In this study, we report the cloning and functional characterization of an early responsive gene, <i>BplERD15</i>, from <i>Betula platyphylla</i> Suk to dehydration. <i>BplERD15</i> is located in the same branch as <i>Morus indica</i> Linnaeus <i>...
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MDPI AG
2020-09-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/9/978 |
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| author | Kaiwen Lv Hairong Wei Jing Jiang |
| author_facet | Kaiwen Lv Hairong Wei Jing Jiang |
| author_sort | Kaiwen Lv |
| collection | DOAJ |
| description | In this study, we report the cloning and functional characterization of an early responsive gene, <i>BplERD15</i>, from <i>Betula platyphylla</i> Suk to dehydration. <i>BplERD15</i> is located in the same branch as <i>Morus indica</i> Linnaeus <i>ERD15</i> and <i>Arabidopsis</i> Heynh <i>ERD15</i> in the phylogenetic tree built with ERD family protein sequences. The tissue-specific expression patterns of <i>BplERD15</i> were characterized using qRT-PCR and the results showed that the transcript levels of <i>BplERD15</i> in six tissues were ranked from the highest to the lowest levels as the following: mature leaves (ML) > young leaves (YL) > roots (R) > buds (B) > young stems (YS) > mature stems (MS). Multiple drought experiments were simulated by adding various osmotica including polyethylene glycol, mannitol, and NaCl to the growth media to decrease their water potentials, and the results showed that the expression of <i>BplERD15</i> could be induced to 12, 9, and 10 folds, respectively, within a 48 h period. However, the expression level of <i>BplERD15</i> was inhibited by the plant hormone abscisic acid in the early response and then restored to the level of control. The <i>BplERD15</i> overexpression (OE) transgenic birch lines were developed and they did not exhibit any phenotypic anomalies and growth deficiency under normal condition. Under drought condition, <i>BplERD15-OE1</i>, <i>3</i>, and <i>4</i> all displayed some drought tolerant characteristics and survived from the drought while the wild type (WT) plants withered and then died. Analysis showed that all <i>BplERD15-OE</i> lines had significant lower electrolyte leakage levels as compared to WT. Our study suggests that <i>BplERD15</i> is a drought-responsive gene that can reduce mortality under stress condition. |
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| language | English |
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| series | Forests |
| spelling | doaj.art-557fc06135e84c2392027a42cd2a66812023-11-20T13:13:38ZengMDPI AGForests1999-49072020-09-0111997810.3390/f11090978Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk.Kaiwen Lv0Hairong Wei1Jing Jiang2State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaCollege of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USAState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaIn this study, we report the cloning and functional characterization of an early responsive gene, <i>BplERD15</i>, from <i>Betula platyphylla</i> Suk to dehydration. <i>BplERD15</i> is located in the same branch as <i>Morus indica</i> Linnaeus <i>ERD15</i> and <i>Arabidopsis</i> Heynh <i>ERD15</i> in the phylogenetic tree built with ERD family protein sequences. The tissue-specific expression patterns of <i>BplERD15</i> were characterized using qRT-PCR and the results showed that the transcript levels of <i>BplERD15</i> in six tissues were ranked from the highest to the lowest levels as the following: mature leaves (ML) > young leaves (YL) > roots (R) > buds (B) > young stems (YS) > mature stems (MS). Multiple drought experiments were simulated by adding various osmotica including polyethylene glycol, mannitol, and NaCl to the growth media to decrease their water potentials, and the results showed that the expression of <i>BplERD15</i> could be induced to 12, 9, and 10 folds, respectively, within a 48 h period. However, the expression level of <i>BplERD15</i> was inhibited by the plant hormone abscisic acid in the early response and then restored to the level of control. The <i>BplERD15</i> overexpression (OE) transgenic birch lines were developed and they did not exhibit any phenotypic anomalies and growth deficiency under normal condition. Under drought condition, <i>BplERD15-OE1</i>, <i>3</i>, and <i>4</i> all displayed some drought tolerant characteristics and survived from the drought while the wild type (WT) plants withered and then died. Analysis showed that all <i>BplERD15-OE</i> lines had significant lower electrolyte leakage levels as compared to WT. Our study suggests that <i>BplERD15</i> is a drought-responsive gene that can reduce mortality under stress condition.https://www.mdpi.com/1999-4907/11/9/978<i>Betula platyphylla</i>early responsive to dehydration genedrought stresstransgenic lines |
| spellingShingle | Kaiwen Lv Hairong Wei Jing Jiang Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. Forests <i>Betula platyphylla</i> early responsive to dehydration gene drought stress transgenic lines |
| title | Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. |
| title_full | Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. |
| title_fullStr | Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. |
| title_full_unstemmed | Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. |
| title_short | Overexpression of <i>BplERD15</i> Enhances Drought Tolerance in <i>Betula platyphylla</i> Suk. |
| title_sort | overexpression of i bplerd15 i enhances drought tolerance in i betula platyphylla i suk |
| topic | <i>Betula platyphylla</i> early responsive to dehydration gene drought stress transgenic lines |
| url | https://www.mdpi.com/1999-4907/11/9/978 |
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