An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar
The subgenus Laurentomantis in the genus Gephyromantis contains some of the least known amphibian species of Madagascar. The six currently valid nominal species are rainforest frogs known from few individuals, hampering a full understanding of the species diversity of the clade. We assembled data on...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft
2022-05-01
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| Series: | Vertebrate Zoology |
| Online Access: | https://vertebrate-zoology.arphahub.com/article/78830/download/pdf/ |
| _version_ | 1797333876493778944 |
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| author | Miguel Vences Jörn Köhler Angelica Crottini Michael Hofreiter Carl R. Hutter Louis du Preez Michaela Preick Andolalao Rakotoarison Loïs Rancilhac Achille P. Raselimanana Gonçalo M. Rosa Mark D. Scherz Frank Glaw |
| author_facet | Miguel Vences Jörn Köhler Angelica Crottini Michael Hofreiter Carl R. Hutter Louis du Preez Michaela Preick Andolalao Rakotoarison Loïs Rancilhac Achille P. Raselimanana Gonçalo M. Rosa Mark D. Scherz Frank Glaw |
| author_sort | Miguel Vences |
| collection | DOAJ |
| description | The subgenus Laurentomantis in the genus Gephyromantis contains some of the least known amphibian species of Madagascar. The six currently valid nominal species are rainforest frogs known from few individuals, hampering a full understanding of the species diversity of the clade. We assembled data on specimens collected during field surveys over the past 30 years and integrated analysis of mitochondrial and nuclear-encoded genes of 88 individuals, a comprehensive bioacoustic analysis, and morphological comparisons to delimit a minimum of nine species-level lineages in the subgenus. To clarify the identity of the species Gephyromantis malagasius, we applied a target-enrichment approach to a sample of the 110 year-old holotype of Microphryne malagasia Methuen and Hewitt, 1913 to assign this specimen to a lineage based on a mitochondrial DNA barcode. The holotype clustered unambiguously with specimens previously named G. ventrimaculatus. Consequently we propose to consider Trachymantis malagasia ventrimaculatus Angel, 1935 as a junior synonym of Gephyromantis malagasius. Due to this redefinition of G. malagasius, no scientific name is available for any of the four deep lineages of frogs previously subsumed under this name, all characterized by red color ventrally on the hindlimbs. These are here formally named as Gephyromantis fiharimpe sp. nov., G. matsilo sp. nov., G. oelkrugi sp. nov., and G. portonae sp. nov. The new species are distinguishable from each other by genetic divergences of >4% uncorrected pairwise distance in a fragment of the 16S rRNA marker and a combination of morphological and bioacoustic characters. Gephyromantis fiharimpe and G. matsilo occur, respectively, at mid-elevations and lower elevations along a wide stretch of Madagascar’s eastern rainforest band, while G. oelkrugi and G. portonae appear to be more range-restricted in parts of Madagascar’s North East and Northern Central East regions. Open taxonomic questions surround G. horridus, to which we here assign specimens from Montagne d’Ambre and the type locality Nosy Be; and G. ranjomavo, which contains genetically divergent populations from Marojejy, Tsaratanana, and Ampotsidy. |
| first_indexed | 2024-03-08T08:11:25Z |
| format | Article |
| id | doaj.art-5580763527ec4756a0106d24ce47ea62 |
| institution | Directory Open Access Journal |
| issn | 2625-8498 |
| language | English |
| last_indexed | 2024-03-08T08:11:25Z |
| publishDate | 2022-05-01 |
| publisher | Pensoft |
| record_format | Article |
| series | Vertebrate Zoology |
| spelling | doaj.art-5580763527ec4756a0106d24ce47ea622024-02-02T08:38:21ZengPensoftVertebrate Zoology2625-84982022-05-017227130910.3897/vz.72.e7883078830An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from MadagascarMiguel Vences0Jörn Köhler1Angelica Crottini2Michael Hofreiter3Carl R. Hutter4Louis du Preez5Michaela Preick6Andolalao Rakotoarison7Loïs Rancilhac8Achille P. Raselimanana9Gonçalo M. Rosa10Mark D. Scherz11Frank Glaw12Technische Universität BraunschweigHessisches LandesmuseumUniversidade do PortoUniversität PotsdamLouisiana State UniversityNorth-West UniversityPotsdam UniversityTechnische Universität BraunschweigTecnische Universitaet BraunschweigUniversité d’AntananarivoZoological Society of LondonUniversity of CopenhagenZoologische Staatssammlung MünchenThe subgenus Laurentomantis in the genus Gephyromantis contains some of the least known amphibian species of Madagascar. The six currently valid nominal species are rainforest frogs known from few individuals, hampering a full understanding of the species diversity of the clade. We assembled data on specimens collected during field surveys over the past 30 years and integrated analysis of mitochondrial and nuclear-encoded genes of 88 individuals, a comprehensive bioacoustic analysis, and morphological comparisons to delimit a minimum of nine species-level lineages in the subgenus. To clarify the identity of the species Gephyromantis malagasius, we applied a target-enrichment approach to a sample of the 110 year-old holotype of Microphryne malagasia Methuen and Hewitt, 1913 to assign this specimen to a lineage based on a mitochondrial DNA barcode. The holotype clustered unambiguously with specimens previously named G. ventrimaculatus. Consequently we propose to consider Trachymantis malagasia ventrimaculatus Angel, 1935 as a junior synonym of Gephyromantis malagasius. Due to this redefinition of G. malagasius, no scientific name is available for any of the four deep lineages of frogs previously subsumed under this name, all characterized by red color ventrally on the hindlimbs. These are here formally named as Gephyromantis fiharimpe sp. nov., G. matsilo sp. nov., G. oelkrugi sp. nov., and G. portonae sp. nov. The new species are distinguishable from each other by genetic divergences of >4% uncorrected pairwise distance in a fragment of the 16S rRNA marker and a combination of morphological and bioacoustic characters. Gephyromantis fiharimpe and G. matsilo occur, respectively, at mid-elevations and lower elevations along a wide stretch of Madagascar’s eastern rainforest band, while G. oelkrugi and G. portonae appear to be more range-restricted in parts of Madagascar’s North East and Northern Central East regions. Open taxonomic questions surround G. horridus, to which we here assign specimens from Montagne d’Ambre and the type locality Nosy Be; and G. ranjomavo, which contains genetically divergent populations from Marojejy, Tsaratanana, and Ampotsidy.https://vertebrate-zoology.arphahub.com/article/78830/download/pdf/ |
| spellingShingle | Miguel Vences Jörn Köhler Angelica Crottini Michael Hofreiter Carl R. Hutter Louis du Preez Michaela Preick Andolalao Rakotoarison Loïs Rancilhac Achille P. Raselimanana Gonçalo M. Rosa Mark D. Scherz Frank Glaw An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar Vertebrate Zoology |
| title | An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar |
| title_full | An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar |
| title_fullStr | An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar |
| title_full_unstemmed | An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar |
| title_short | An integrative taxonomic revision and redefinition of Gephyromantis (Laurentomantis) malagasius based on archival DNA analysis reveals four new mantellid frog species from Madagascar |
| title_sort | integrative taxonomic revision and redefinition of gephyromantis laurentomantis malagasius based on archival dna analysis reveals four new mantellid frog species from madagascar |
| url | https://vertebrate-zoology.arphahub.com/article/78830/download/pdf/ |
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