Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.)
The modification of secondary metabolites is crucial to the function of metabolites in tea (<i>Camellia sinensis</i> L.). The arabinan deficient (ARAD) encodes an arabinosyltransferase and is involved in the arabinan biosynthesis pathway. Two full-length sequences of CsARADs were cloned...
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MDPI AG
2023-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/6/1476 |
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| author | Tzu-Chiao Liao Chung-Tse Chen Mao-Chang Wang Shang-Ling Ou Jason T. C. Tzen Chin-Ying Yang |
| author_facet | Tzu-Chiao Liao Chung-Tse Chen Mao-Chang Wang Shang-Ling Ou Jason T. C. Tzen Chin-Ying Yang |
| author_sort | Tzu-Chiao Liao |
| collection | DOAJ |
| description | The modification of secondary metabolites is crucial to the function of metabolites in tea (<i>Camellia sinensis</i> L.). The arabinan deficient (ARAD) encodes an arabinosyltransferase and is involved in the arabinan biosynthesis pathway. Two full-length sequences of CsARADs were cloned and obtained from tea plants through the rapid amplification of cDNA ends and named CsARAD1 and CsARAD2. CsARAD1 and CsARAD2 are predicted to be 2 membrane proteins containing <i>N</i>-glycosylation, phosphorylation, and <i>N</i>-myristoylation sites and are 2 homologs of the glycosyltransferases (GT) 47 family, according to various bioinformatic analyses. CsARADs showed higher transcription levels in nonlignified tissues (e.g., buds and young leaves) than in old leaves and stems. CsARADs also exhibited the highest expression level in autumn, indicating that CsARAD regulation is affected by environmental factors. The transcript levels of CsARADs were changed after various abiotic stress treatments, and CsARAD1 and CsARAD2 displayed different regulation patterns in temperature stress, saline, and drought-like conditions. CsARAD1 and CsARAD2 were both significantly downregulated after tea seedlings were treated with an ethylene precursor and abscisic acid. In addition, CsARAD2 was downregulated after being treated with methyl jasmonate and gibberellin. Collectively, our findings on the function of arabinosyltransferase serve as a basis for further research and breeding applications. |
| first_indexed | 2024-03-11T02:52:44Z |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T02:52:44Z |
| publishDate | 2023-05-01 |
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| series | Agronomy |
| spelling | doaj.art-5582ddfdc9ef41ca87a50af3f194624a2023-11-18T08:53:48ZengMDPI AGAgronomy2073-43952023-05-01136147610.3390/agronomy13061476Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.)Tzu-Chiao Liao0Chung-Tse Chen1Mao-Chang Wang2Shang-Ling Ou3Jason T. C. Tzen4Chin-Ying Yang5Department of Agronomy, National Chung Hsing University, Taichung 40227, TaiwanGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Accounting, Chinese Culture University, Taipei 11114, TaiwanDepartment of Agronomy, National Chung Hsing University, Taichung 40227, TaiwanGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Agronomy, National Chung Hsing University, Taichung 40227, TaiwanThe modification of secondary metabolites is crucial to the function of metabolites in tea (<i>Camellia sinensis</i> L.). The arabinan deficient (ARAD) encodes an arabinosyltransferase and is involved in the arabinan biosynthesis pathway. Two full-length sequences of CsARADs were cloned and obtained from tea plants through the rapid amplification of cDNA ends and named CsARAD1 and CsARAD2. CsARAD1 and CsARAD2 are predicted to be 2 membrane proteins containing <i>N</i>-glycosylation, phosphorylation, and <i>N</i>-myristoylation sites and are 2 homologs of the glycosyltransferases (GT) 47 family, according to various bioinformatic analyses. CsARADs showed higher transcription levels in nonlignified tissues (e.g., buds and young leaves) than in old leaves and stems. CsARADs also exhibited the highest expression level in autumn, indicating that CsARAD regulation is affected by environmental factors. The transcript levels of CsARADs were changed after various abiotic stress treatments, and CsARAD1 and CsARAD2 displayed different regulation patterns in temperature stress, saline, and drought-like conditions. CsARAD1 and CsARAD2 were both significantly downregulated after tea seedlings were treated with an ethylene precursor and abscisic acid. In addition, CsARAD2 was downregulated after being treated with methyl jasmonate and gibberellin. Collectively, our findings on the function of arabinosyltransferase serve as a basis for further research and breeding applications.https://www.mdpi.com/2073-4395/13/6/1476tea<i>Camellia sinensis</i> L.arabinan deficientARADabiotic stress |
| spellingShingle | Tzu-Chiao Liao Chung-Tse Chen Mao-Chang Wang Shang-Ling Ou Jason T. C. Tzen Chin-Ying Yang Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) Agronomy tea <i>Camellia sinensis</i> L. arabinan deficient ARAD abiotic stress |
| title | Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) |
| title_full | Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) |
| title_fullStr | Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) |
| title_full_unstemmed | Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) |
| title_short | Isolation and Molecular Characterization of Two Arabinosyltransferases in Response to Abiotic Stresses in Sijichun Tea Plants (<i>Camellia sinensis</i> L.) |
| title_sort | isolation and molecular characterization of two arabinosyltransferases in response to abiotic stresses in sijichun tea plants i camellia sinensis i l |
| topic | tea <i>Camellia sinensis</i> L. arabinan deficient ARAD abiotic stress |
| url | https://www.mdpi.com/2073-4395/13/6/1476 |
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