Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets
Abstract We aimed to compare two assessment methodologies (real-time vs. video-recorded) using the Unesp-Botucatu Pig Composite Acute Pain Scale (UPAPS) in piglets before and after castration. Twenty-nine male piglets were castrated. Four observers scored the UPAPS over three perioperative timepoint...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45869-8 |
| _version_ | 1797636960837173248 |
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| author | Pedro Henrique Esteves Trindade Magdiel Lopez-Soriano Victoria Rocha Merenda Rubia Mitalli Tomacheuski Monique Danielle Pairis-Garcia |
| author_facet | Pedro Henrique Esteves Trindade Magdiel Lopez-Soriano Victoria Rocha Merenda Rubia Mitalli Tomacheuski Monique Danielle Pairis-Garcia |
| author_sort | Pedro Henrique Esteves Trindade |
| collection | DOAJ |
| description | Abstract We aimed to compare two assessment methodologies (real-time vs. video-recorded) using the Unesp-Botucatu Pig Composite Acute Pain Scale (UPAPS) in piglets before and after castration. Twenty-nine male piglets were castrated. Four observers scored the UPAPS over three perioperative timepoints of castration following two assessment methodologies. In real-time assessments, the observers were in-person observing the piglets in front of the pen. After two weeks, the observers did video-recorded assessments randomizing piglets and timepoints. Modeling was conducted to compare the UPAPS and each pain-altered behavior between methodologies. Intraclass correlation coefficient (ICC), Bland–Altman, and Lin’s concordance correlation coefficient (CCC) were conducted to investigate agreement between methodologies. UPAPS was statistically equivalent between methodologies (P = 0.4371). The ICC for each method was very good (0.85 to 0.91). The agreement of the UPAPS assessed between methodologies had minimal bias (− 0.04), no proportion bias, and 53% of the assessments presented a perfect agreement. However, CCC of the UPAPS was moderate (0.65), and only one pain-altered behavior (“presents difficulty in overcoming obstacles or other animals”) occurred more in real-time assessments (P = 0.0444). In conclusion, piglet pain assessment by UPAPS can be conducted in real-time based on a suitable agreement between the real-time and video-recorded assessment methods. |
| first_indexed | 2024-03-11T12:42:38Z |
| format | Article |
| id | doaj.art-55850200e1314980895a184f96c5b147 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T12:42:38Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-55850200e1314980895a184f96c5b1472023-11-05T12:12:30ZengNature PortfolioScientific Reports2045-23222023-10-011311810.1038/s41598-023-45869-8Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated pigletsPedro Henrique Esteves Trindade0Magdiel Lopez-Soriano1Victoria Rocha Merenda2Rubia Mitalli Tomacheuski3Monique Danielle Pairis-Garcia4Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU)Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU)Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU)Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU)Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU)Abstract We aimed to compare two assessment methodologies (real-time vs. video-recorded) using the Unesp-Botucatu Pig Composite Acute Pain Scale (UPAPS) in piglets before and after castration. Twenty-nine male piglets were castrated. Four observers scored the UPAPS over three perioperative timepoints of castration following two assessment methodologies. In real-time assessments, the observers were in-person observing the piglets in front of the pen. After two weeks, the observers did video-recorded assessments randomizing piglets and timepoints. Modeling was conducted to compare the UPAPS and each pain-altered behavior between methodologies. Intraclass correlation coefficient (ICC), Bland–Altman, and Lin’s concordance correlation coefficient (CCC) were conducted to investigate agreement between methodologies. UPAPS was statistically equivalent between methodologies (P = 0.4371). The ICC for each method was very good (0.85 to 0.91). The agreement of the UPAPS assessed between methodologies had minimal bias (− 0.04), no proportion bias, and 53% of the assessments presented a perfect agreement. However, CCC of the UPAPS was moderate (0.65), and only one pain-altered behavior (“presents difficulty in overcoming obstacles or other animals”) occurred more in real-time assessments (P = 0.0444). In conclusion, piglet pain assessment by UPAPS can be conducted in real-time based on a suitable agreement between the real-time and video-recorded assessment methods.https://doi.org/10.1038/s41598-023-45869-8 |
| spellingShingle | Pedro Henrique Esteves Trindade Magdiel Lopez-Soriano Victoria Rocha Merenda Rubia Mitalli Tomacheuski Monique Danielle Pairis-Garcia Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets Scientific Reports |
| title | Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets |
| title_full | Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets |
| title_fullStr | Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets |
| title_full_unstemmed | Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets |
| title_short | Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets |
| title_sort | effects of assessment method real time versus video recorded on a validated pain altered behavior scale used in castrated piglets |
| url | https://doi.org/10.1038/s41598-023-45869-8 |
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