Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)
To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were id...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01192/full |
| _version_ | 1828752382569218048 |
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| author | Xiaochuan Sun Xiaochuan Sun Xiaochuan Sun Yan Wang Yan Wang Liang Xu Liang Xu Chao Li Wei Zhang Xiaobo Luo Xiaobo Luo Haiyan Jiang Liwang Liu Liwang Liu |
| author_facet | Xiaochuan Sun Xiaochuan Sun Xiaochuan Sun Yan Wang Yan Wang Liang Xu Liang Xu Chao Li Wei Zhang Xiaobo Luo Xiaobo Luo Haiyan Jiang Liwang Liu Liwang Liu |
| author_sort | Xiaochuan Sun |
| collection | DOAJ |
| description | To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops. |
| first_indexed | 2024-12-10T21:08:58Z |
| format | Article |
| id | doaj.art-558ec9f5720c4c1b86b97df107797698 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T21:08:58Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-558ec9f5720c4c1b86b97df1077976982022-12-22T01:33:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01192260921Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)Xiaochuan Sun0Xiaochuan Sun1Xiaochuan Sun2Yan Wang3Yan Wang4Liang Xu5Liang Xu6Chao Li7Wei Zhang8Xiaobo Luo9Xiaobo Luo10Haiyan Jiang11Liwang Liu12Liwang Liu13National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of TechnologyHuai'an, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityNanjing, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing, ChinaTo understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.http://journal.frontiersin.org/article/10.3389/fpls.2017.01192/fullradishsalt stressiTRAQproteomicsassociation analysis |
| spellingShingle | Xiaochuan Sun Xiaochuan Sun Xiaochuan Sun Yan Wang Yan Wang Liang Xu Liang Xu Chao Li Wei Zhang Xiaobo Luo Xiaobo Luo Haiyan Jiang Liwang Liu Liwang Liu Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) Frontiers in Plant Science radish salt stress iTRAQ proteomics association analysis |
| title | Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) |
| title_full | Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) |
| title_fullStr | Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) |
| title_full_unstemmed | Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) |
| title_short | Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.) |
| title_sort | unraveling the root proteome changes and its relationship to molecular mechanism underlying salt stress response in radish raphanus sativus l |
| topic | radish salt stress iTRAQ proteomics association analysis |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01192/full |
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