APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
1222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2012-05-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/905 |
| _version_ | 1797237685008465920 |
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| author | I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev |
| author_facet | I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev |
| author_sort | I. A. Pashnina |
| collection | DOAJ |
| description | 1222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory comparability of different laboratory methods. The most sensitive method for detection of antinuclear antibodies is indirect immunofluorescence. This is a preferable method for screening of autoimmune connective tissue diseases. At comparison of luminescence types in immunofluorescence test and results of immunoblotting was shown that for each type of a luminescence the set of antibody, revealed by immunoblotting, was characteristic. However, the same antibodies can be found in various types of fluorescence that complicates unequivocal interpretation of immunofluorescence test results. Antibodies to Ro-52 were most often found in all types of fluorescence, and also in the absence of that. |
| first_indexed | 2024-03-08T12:23:02Z |
| format | Article |
| id | doaj.art-559191a0e9af472a837725fbd40564a4 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:23:40Z |
| publishDate | 2012-05-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-559191a0e9af472a837725fbd40564a42024-03-28T07:49:04ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962012-05-0103(2)143147851APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASESI. A. Pashnina0I. M. Krivolapova1I. A. Tuzankina2V. A. Chereshnev3Institute of Immunology and Physiology, Urals Branch of the Russian Acad. Sci.Institute of Immunology and Physiology, Urals Branch of the Russian Acad. Sci.Institute of Immunology and Physiology, Urals Branch of the Russian Acad. Sci.Institute of Immunology and Physiology, Urals Branch of the Russian Acad. Sci.1222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory comparability of different laboratory methods. The most sensitive method for detection of antinuclear antibodies is indirect immunofluorescence. This is a preferable method for screening of autoimmune connective tissue diseases. At comparison of luminescence types in immunofluorescence test and results of immunoblotting was shown that for each type of a luminescence the set of antibody, revealed by immunoblotting, was characteristic. However, the same antibodies can be found in various types of fluorescence that complicates unequivocal interpretation of immunofluorescence test results. Antibodies to Ro-52 were most often found in all types of fluorescence, and also in the absence of that.https://www.actabiomedica.ru/jour/article/view/905autoantibodiesautoimmune diseases |
| spellingShingle | I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES Acta Biomedica Scientifica autoantibodies autoimmune diseases |
| title | APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES |
| title_full | APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES |
| title_fullStr | APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES |
| title_full_unstemmed | APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES |
| title_short | APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES |
| title_sort | application of different laboratory methods for antinuclear autoantibodies investigation in patients with autoimmune connective tissue diseases |
| topic | autoantibodies autoimmune diseases |
| url | https://www.actabiomedica.ru/jour/article/view/905 |
| work_keys_str_mv | AT iapashnina applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT imkrivolapova applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT iatuzankina applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT vachereshnev applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases |