Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane
Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-<i>co</i>-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for th...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/24/3/508 |
| _version_ | 1817980446206590976 |
|---|---|
| author | William J. Cloete Stefan Hayward Pieter Swart Bert Klumperman |
| author_facet | William J. Cloete Stefan Hayward Pieter Swart Bert Klumperman |
| author_sort | William J. Cloete |
| collection | DOAJ |
| description | Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-<i>co</i>-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone. |
| first_indexed | 2024-04-13T22:53:55Z |
| format | Article |
| id | doaj.art-55935b63feb64aefaab8d2ce58669a4b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T22:53:55Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-55935b63feb64aefaab8d2ce58669a4b2022-12-22T02:26:05ZengMDPI AGMolecules1420-30492019-01-0124350810.3390/molecules24030508molecules24030508Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous MembraneWilliam J. Cloete0Stefan Hayward1Pieter Swart2Bert Klumperman3Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaDepartment of Biochemistry, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaDepartment of Biochemistry, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaDepartment of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland 7602, South AfricaTwo commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-<i>co</i>-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone.https://www.mdpi.com/1420-3049/24/3/508poly(styrene-co-maleic anhydride)enzymeimmobilizationprotease |
| spellingShingle | William J. Cloete Stefan Hayward Pieter Swart Bert Klumperman Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane Molecules poly(styrene-co-maleic anhydride) enzyme immobilization protease |
| title | Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane |
| title_full | Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane |
| title_fullStr | Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane |
| title_full_unstemmed | Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane |
| title_short | Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane |
| title_sort | degradation of proteins and starch by combined immobilization of protease α amylase and β galactosidase on a single electrospun nanofibrous membrane |
| topic | poly(styrene-co-maleic anhydride) enzyme immobilization protease |
| url | https://www.mdpi.com/1420-3049/24/3/508 |
| work_keys_str_mv | AT williamjcloete degradationofproteinsandstarchbycombinedimmobilizationofproteaseaamylaseandbgalactosidaseonasingleelectrospunnanofibrousmembrane AT stefanhayward degradationofproteinsandstarchbycombinedimmobilizationofproteaseaamylaseandbgalactosidaseonasingleelectrospunnanofibrousmembrane AT pieterswart degradationofproteinsandstarchbycombinedimmobilizationofproteaseaamylaseandbgalactosidaseonasingleelectrospunnanofibrousmembrane AT bertklumperman degradationofproteinsandstarchbycombinedimmobilizationofproteaseaamylaseandbgalactosidaseonasingleelectrospunnanofibrousmembrane |