| Summary: | Chilean peppers, cultivated from <i>Capsicum pubescens</i>, are globally renowned as popular vegetable and spice crops. <i>C. pubescens</i> belongs to the <i>Capsicum</i> L. (pepper) family and is one of the five pepper cultivars grown in China. In this study, we assembled and annotated the complete mt genome of <i>C. pubescens</i>. We investigated several aspects of its genome, including characteristics, codon usage, RNA editing sites, repeat sequences, selective pressure, gene clusters, and phylogenetic relationships. Furthermore, we compared it with other plant mt genomes. The data we obtained will provide valuable information for studying evolutionary processes in the Capsicum genus and will assist in the functional analysis of Capsicum mitogenomes.
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