Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells
Introduction: Cardiovascular disease (CVD) is a type of disease that affects the function of cardiac-vascular tissues. This study aimed to consider the possible effects of autophagy, as an intrinsic catabolic pathway of cells, on the differentiation and aging process of mesenchymal stem cells (MSCs)...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-08-01
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| Series: | Journal of Cardiovascular and Thoracic Research |
| Subjects: | |
| Online Access: | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-234.pdf |
| _version_ | 1818364141646118912 |
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| author | Mehdi Hassanpour Omid Cheraghi Reza Rahbarghazi Mohammad Nouri |
| author_facet | Mehdi Hassanpour Omid Cheraghi Reza Rahbarghazi Mohammad Nouri |
| author_sort | Mehdi Hassanpour |
| collection | DOAJ |
| description | Introduction: Cardiovascular disease (CVD) is a type of disease that affects the function of cardiac-vascular tissues. This study aimed to consider the possible effects of autophagy, as an intrinsic catabolic pathway of cells, on the differentiation and aging process of mesenchymal stem cells (MSCs). Methods: In this study, bone marrow-derived MSCs were obtained from rabbit bone marrow aspirates. The stemness feature was confirmed by using flow cytometry analysis Cells at passage three were treated with 50 μM Metformin and 15μM hydroxychloroquine (HCQ) for 72 hours. The intracellular accumulation of autophagolysosomes was imaged using LysoTracker staining. Protein levels of autophagy (LC3II/I ratio), aging (Klotho, PARP-1, and Sirt-1) effectors, and cardiomyocyte-like phenotype (α-actinin) were studied by western blotting. Results: Based on our findings, flow cytometry analysis showed that the obtained cells expressed CD44 and CD133 strongly, and CD31 and CD34 dimly, showing a typical characteristic of MSCs. Our data confirmed an increased LC3II/I ratio in the metformin-received group compared to the untreated and HCQ-treated cells (P < 0.05). Besides, we showed that the incubation of rabbit MSCs with HCQ increased cellular aging by induction of PARP-1 while Metformin increased rejuvenating factor Sirt-1 comparing with the normal group (P < 0.05). Western blotting data showed that the autophagy stimulation response in rabbit MSCs postponed the biological aging and decreased the differentiation potential to the cardiac cells by diminishing α-actinin comparing with control cells (P < 0.05). Conclusion: In summary, for the informants in this study, it could be noted that autophagy inhibition/stimulation could alter rabbit MSCs aging and differentiation capacity. |
| first_indexed | 2024-12-13T21:59:39Z |
| format | Article |
| id | doaj.art-5594ed6ea10146e79d7c6a3c367e8946 |
| institution | Directory Open Access Journal |
| issn | 2008-5117 2008-6830 |
| language | English |
| last_indexed | 2024-12-13T21:59:39Z |
| publishDate | 2021-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Cardiovascular and Thoracic Research |
| spelling | doaj.art-5594ed6ea10146e79d7c6a3c367e89462022-12-21T23:30:03ZengTabriz University of Medical SciencesJournal of Cardiovascular and Thoracic Research2008-51172008-68302021-08-0113323424010.34172/jcvtr.2021.43jcvtr-30312Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cellsMehdi Hassanpour0Omid Cheraghi1Reza Rahbarghazi2Mohammad Nouri3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, IranDepartment of Applied Cell Science, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, IranIntroduction: Cardiovascular disease (CVD) is a type of disease that affects the function of cardiac-vascular tissues. This study aimed to consider the possible effects of autophagy, as an intrinsic catabolic pathway of cells, on the differentiation and aging process of mesenchymal stem cells (MSCs). Methods: In this study, bone marrow-derived MSCs were obtained from rabbit bone marrow aspirates. The stemness feature was confirmed by using flow cytometry analysis Cells at passage three were treated with 50 μM Metformin and 15μM hydroxychloroquine (HCQ) for 72 hours. The intracellular accumulation of autophagolysosomes was imaged using LysoTracker staining. Protein levels of autophagy (LC3II/I ratio), aging (Klotho, PARP-1, and Sirt-1) effectors, and cardiomyocyte-like phenotype (α-actinin) were studied by western blotting. Results: Based on our findings, flow cytometry analysis showed that the obtained cells expressed CD44 and CD133 strongly, and CD31 and CD34 dimly, showing a typical characteristic of MSCs. Our data confirmed an increased LC3II/I ratio in the metformin-received group compared to the untreated and HCQ-treated cells (P < 0.05). Besides, we showed that the incubation of rabbit MSCs with HCQ increased cellular aging by induction of PARP-1 while Metformin increased rejuvenating factor Sirt-1 comparing with the normal group (P < 0.05). Western blotting data showed that the autophagy stimulation response in rabbit MSCs postponed the biological aging and decreased the differentiation potential to the cardiac cells by diminishing α-actinin comparing with control cells (P < 0.05). Conclusion: In summary, for the informants in this study, it could be noted that autophagy inhibition/stimulation could alter rabbit MSCs aging and differentiation capacity.https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-234.pdfbone marrow mesenchymal stem cellsautophagydifferentiationcardiomyocyteaging |
| spellingShingle | Mehdi Hassanpour Omid Cheraghi Reza Rahbarghazi Mohammad Nouri Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells Journal of Cardiovascular and Thoracic Research bone marrow mesenchymal stem cells autophagy differentiation cardiomyocyte aging |
| title | Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| title_full | Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| title_fullStr | Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| title_full_unstemmed | Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| title_short | Autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| title_sort | autophagy stimulation delayed biological aging and decreased cardiac differentiation in rabbit mesenchymal stem cells |
| topic | bone marrow mesenchymal stem cells autophagy differentiation cardiomyocyte aging |
| url | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-234.pdf |
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