Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle
Background: Sarcocystis is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of Sarcocystis is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of Sarcocystis spp. in heart of cattle in Tehran, Ir...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-12-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2282 |
| _version_ | 1798003967397986304 |
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| author | Ali Dalir Ghaffari Abdolhossein Dalimi Majid Pirestani |
| author_facet | Ali Dalir Ghaffari Abdolhossein Dalimi Majid Pirestani |
| author_sort | Ali Dalir Ghaffari |
| collection | DOAJ |
| description |
Background: Sarcocystis is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of Sarcocystis is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of Sarcocystis spp. in heart of cattle in Tehran, Iran by microscopy and PCR-RFLP methods.
Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing.
Results: Overall, 97 of 100 of the heart muscle samples were infected with Sarcocystis. All the samples were detected as S. cruzi through similar patterns in PCR-RFLP.
Conclusion: S. cruzi is the most common species in the heart of cattle slaughtered in Shahriar.
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| first_indexed | 2024-04-11T12:16:01Z |
| format | Article |
| id | doaj.art-5597d40d6fbb4356897f9f4569cddb07 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T12:16:01Z |
| publishDate | 2022-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-5597d40d6fbb4356897f9f4569cddb072022-12-22T04:24:19ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2022-12-0117410.18502/ijpa.v17i4.11281Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of CattleAli Dalir Ghaffari0Abdolhossein Dalimi1Majid Pirestani2Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: Sarcocystis is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of Sarcocystis is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of Sarcocystis spp. in heart of cattle in Tehran, Iran by microscopy and PCR-RFLP methods. Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing. Results: Overall, 97 of 100 of the heart muscle samples were infected with Sarcocystis. All the samples were detected as S. cruzi through similar patterns in PCR-RFLP. Conclusion: S. cruzi is the most common species in the heart of cattle slaughtered in Shahriar. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2282ProtozoaParasiteCysts |
| spellingShingle | Ali Dalir Ghaffari Abdolhossein Dalimi Majid Pirestani Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle Iranian Journal of Parasitology Protozoa Parasite Cysts |
| title | Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle |
| title_full | Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle |
| title_fullStr | Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle |
| title_full_unstemmed | Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle |
| title_short | Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle |
| title_sort | microscopic and molecular detection of sarcocystis cruzi apicomplexa sarcocystidae in the heart muscle of cattle |
| topic | Protozoa Parasite Cysts |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2282 |
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