Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions
<i>Listeria monocytogenes</i> is a one of the most important food-borne pathogens. Its ability to form biofilm contributes to increased resistance to disinfectants and inefficient disinfection, posing a serious threat for the food industry, and in the end the consumer. The aim of this st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/9/280 |
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| author | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Piotr Gajewski Natalia Wiktorczyk Magdalena Wietlicka-Piszcz Andżelika Dudek Karolina Jadwiga Skowron Eugenia Gospodarek-Komkowska |
| author_facet | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Piotr Gajewski Natalia Wiktorczyk Magdalena Wietlicka-Piszcz Andżelika Dudek Karolina Jadwiga Skowron Eugenia Gospodarek-Komkowska |
| author_sort | Krzysztof Skowron |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> is a one of the most important food-borne pathogens. Its ability to form biofilm contributes to increased resistance to disinfectants and inefficient disinfection, posing a serious threat for the food industry, and in the end the consumer. The aim of this study was the comparison of the biofilm formation ability of <i>L. monocytogenes</i> strains on stainless steel, under different environmental conditions (temperature, pH, NaCl concentration, nutrients availability), and the assessment of biofilm susceptibility to disinfectants. The bactericidal activity of four disinfectants in two concentrations (100% and 50% of working solution) against biofilm was conducted on four clinical strains, four strains isolated from food and one reference strain ATCC 19111. It was found that biofilm susceptibility to disinfectants was influenced by environmental conditions. Biofilm susceptibility correlated with the decrease of temperature, pH, nutrients availability and salinity of the environment. The least sensitive to disinfectants was biofilm produced at pH = 4 (the bacterial number ranged from 0.25 log CFU × cm<sup>−2</sup> to 1.72 log CFU × cm<sup>−2</sup>) whereas the most sensitive was biofilm produced at pH = 9 (5.16 log CFU × cm<sup>−2</sup> to 7.84 log CFU × cm<sup>−2</sup>). Quatosept was the most effective disinfectant, regardless of the conditions. In conclusion, biofilm susceptibility to disinfectants is strain-dependent and is affected by environmental conditions. |
| first_indexed | 2024-12-19T03:23:52Z |
| format | Article |
| id | doaj.art-5599eeae54624db1aa1a6f9f94e4e224 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-19T03:23:52Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5599eeae54624db1aa1a6f9f94e4e2242022-12-21T20:37:42ZengMDPI AGMicroorganisms2076-26072019-08-017928010.3390/microorganisms7090280microorganisms7090280Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental ConditionsKrzysztof Skowron0Ewa Wałecka-Zacharska1Katarzyna Grudlewska2Piotr Gajewski3Natalia Wiktorczyk4Magdalena Wietlicka-Piszcz5Andżelika Dudek6Karolina Jadwiga Skowron7Eugenia Gospodarek-Komkowska8Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 50-375 Wrocław, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Theoretical Foundations of Biomedical Sciences and Medical Computer Science, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandFaculty of Telecommunication, Information Technology and Electrical Engineering, Institute of Telecommunications and Computer Science, UTP University of Science and Technology, Al. prof. S. Kaliskiego 7, 85-796 Bydgoszcz, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, Poland<i>Listeria monocytogenes</i> is a one of the most important food-borne pathogens. Its ability to form biofilm contributes to increased resistance to disinfectants and inefficient disinfection, posing a serious threat for the food industry, and in the end the consumer. The aim of this study was the comparison of the biofilm formation ability of <i>L. monocytogenes</i> strains on stainless steel, under different environmental conditions (temperature, pH, NaCl concentration, nutrients availability), and the assessment of biofilm susceptibility to disinfectants. The bactericidal activity of four disinfectants in two concentrations (100% and 50% of working solution) against biofilm was conducted on four clinical strains, four strains isolated from food and one reference strain ATCC 19111. It was found that biofilm susceptibility to disinfectants was influenced by environmental conditions. Biofilm susceptibility correlated with the decrease of temperature, pH, nutrients availability and salinity of the environment. The least sensitive to disinfectants was biofilm produced at pH = 4 (the bacterial number ranged from 0.25 log CFU × cm<sup>−2</sup> to 1.72 log CFU × cm<sup>−2</sup>) whereas the most sensitive was biofilm produced at pH = 9 (5.16 log CFU × cm<sup>−2</sup> to 7.84 log CFU × cm<sup>−2</sup>). Quatosept was the most effective disinfectant, regardless of the conditions. In conclusion, biofilm susceptibility to disinfectants is strain-dependent and is affected by environmental conditions.https://www.mdpi.com/2076-2607/7/9/280<i>L. monocytogenes</i>biofilm formationbiofilm resistancedisinfectantsstainless steelenvironmental conditions |
| spellingShingle | Krzysztof Skowron Ewa Wałecka-Zacharska Katarzyna Grudlewska Piotr Gajewski Natalia Wiktorczyk Magdalena Wietlicka-Piszcz Andżelika Dudek Karolina Jadwiga Skowron Eugenia Gospodarek-Komkowska Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions Microorganisms <i>L. monocytogenes</i> biofilm formation biofilm resistance disinfectants stainless steel environmental conditions |
| title | Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions |
| title_full | Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions |
| title_fullStr | Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions |
| title_full_unstemmed | Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions |
| title_short | Disinfectant Susceptibility of Biofilm Formed by <i>Listeria monocytogenes</i> under Selected Environmental Conditions |
| title_sort | disinfectant susceptibility of biofilm formed by i listeria monocytogenes i under selected environmental conditions |
| topic | <i>L. monocytogenes</i> biofilm formation biofilm resistance disinfectants stainless steel environmental conditions |
| url | https://www.mdpi.com/2076-2607/7/9/280 |
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