Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children

Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children

ObjectivesTo develop a rapid and low-cost method for 16S rDNA nanopore sequencing.MethodsThis was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify the...

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Bibliographic Details
Main Authors: Yinghu Chen, Lingfeng Mao, Dengming Lai, Weize Xu, Yuebai Zhang, Sihao Wu, Di Yang, Shaobo Zhao, Zhicong Liu, Yi Xiao, Yi Tang, Xiaofang Meng, Min Wang, Jueliang Shi, Qixing Chen, Qiang Shu
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-01-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
nanopore sequencing
16S rDNA
bacterial pneumonia
BALF
prospective study
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2022.1001607/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2022.1001607/full

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