Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production
Abstract Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure is an agricultural waste widely distributed worldwide. This study investigated the induction of cellulases by cow manure and the conversion of...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-11-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0720-2 |
| _version_ | 1818320828748529664 |
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| author | Qin Yan Xinli Liu Yanan Wang Hongxing Li Zhigang Li Lin Zhou Yinbo Qu Zhonghai Li Xiaoming Bao |
| author_facet | Qin Yan Xinli Liu Yanan Wang Hongxing Li Zhigang Li Lin Zhou Yinbo Qu Zhonghai Li Xiaoming Bao |
| author_sort | Qin Yan |
| collection | DOAJ |
| description | Abstract Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure is an agricultural waste widely distributed worldwide. This study investigated the induction of cellulases by cow manure and the conversion of cow manure materials into lignocellulosic ethanol. Alkaline NaOH pretreatment improved the accessibility of cow manure lignocellulose to enzymes followed by enzymatic hydrolysis using Penicillium oxalicum cellulases. The ethanol yields from pretreated cow manure and anaerobically digested cow manure were 0.19 and 0.13 g/g-raw biomass, respectively, using recombinant Saccharomyces cerevisiae strain LF1 designed for lignocellulosic ethanol production through simultaneous saccharification and fermentation. Fed-batch supplementation with cellulolytic enzymes and substrates after initial enzymatic hydrolysis also contributed to ethanol production up to 25.65 g/L. These results demonstrate that cow manure is a potential feedstock for inducing fungal cellulase expression and converting lignocellulose into bioethanol. |
| first_indexed | 2024-12-13T10:31:13Z |
| format | Article |
| id | doaj.art-55a003c06a4f4f258a4090fadacb6253 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T10:31:13Z |
| publishDate | 2018-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-55a003c06a4f4f258a4090fadacb62532022-12-21T23:50:51ZengSpringerOpenAMB Express2191-08552018-11-018111210.1186/s13568-018-0720-2Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol productionQin Yan0Xinli Liu1Yanan Wang2Hongxing Li3Zhigang Li4Lin Zhou5Yinbo Qu6Zhonghai Li7Xiaoming Bao8Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of SciencesState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of SciencesShandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of SciencesShandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of SciencesShandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of SciencesShandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qi Lu University of Technology, Shandong Academy of SciencesState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of SciencesState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of SciencesAbstract Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure is an agricultural waste widely distributed worldwide. This study investigated the induction of cellulases by cow manure and the conversion of cow manure materials into lignocellulosic ethanol. Alkaline NaOH pretreatment improved the accessibility of cow manure lignocellulose to enzymes followed by enzymatic hydrolysis using Penicillium oxalicum cellulases. The ethanol yields from pretreated cow manure and anaerobically digested cow manure were 0.19 and 0.13 g/g-raw biomass, respectively, using recombinant Saccharomyces cerevisiae strain LF1 designed for lignocellulosic ethanol production through simultaneous saccharification and fermentation. Fed-batch supplementation with cellulolytic enzymes and substrates after initial enzymatic hydrolysis also contributed to ethanol production up to 25.65 g/L. These results demonstrate that cow manure is a potential feedstock for inducing fungal cellulase expression and converting lignocellulose into bioethanol.http://link.springer.com/article/10.1186/s13568-018-0720-2Cow manureBioethanolCellulasePenicillium oxalicum |
| spellingShingle | Qin Yan Xinli Liu Yanan Wang Hongxing Li Zhigang Li Lin Zhou Yinbo Qu Zhonghai Li Xiaoming Bao Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production AMB Express Cow manure Bioethanol Cellulase Penicillium oxalicum |
| title | Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| title_full | Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| title_fullStr | Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| title_full_unstemmed | Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| title_short | Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| title_sort | cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production |
| topic | Cow manure Bioethanol Cellulase Penicillium oxalicum |
| url | http://link.springer.com/article/10.1186/s13568-018-0720-2 |
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