Biological significance of miR-126 expression in atrial fibrillation and heart failure
We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 m...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2015-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/bjmbr/v48n11/1414-431X-bjmbr-1414-431X20154590.pdf |
| _version_ | 1798013859272851456 |
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| author | X.J. Wei M. Han F.Y. Yang G.C. Wei Z.G. Liang H. Yao C.W. Ji R.S. Xie C.L. Gong Y. Tian |
| author_facet | X.J. Wei M. Han F.Y. Yang G.C. Wei Z.G. Liang H. Yao C.W. Ji R.S. Xie C.L. Gong Y. Tian |
| author_sort | X.J. Wei |
| collection | DOAJ |
| description | We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2−ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those with paroxysmal AF (P<0.05, t-test). Moreover, miR-126 expression was markedly lower in the HF-AF group compared with the AF and HF groups. The 3 patient groups had higher N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, lower left ventricular ejection fraction (LVEF), larger left atrial diameter, and higher cardiothoracic ratio compared with controls. There were significant differences in NT-proBNP levels and LVEF among the AF, HF, and HF-AF groups. Pearson correlation analysis showed that relative miR-126 expression was positively associated with LVEF, logarithm of NT-proBNP, left atrial diameter, cardiothoracic ratio, and age in HF-AF patients. Multiple linear regression analysis showed that miR-126 expression was positively correlated with LVEF, but negatively correlated with the logarithm of NT-pro BNP and the cardiothoracic ratio (all P<0.05). Serum miR-126 levels could serve as a potential candidate biomarker for evaluating the severity of AF and HF. However, to confirm these results, future studies with a larger and diverse patient population are necessary. |
| first_indexed | 2024-04-11T15:09:19Z |
| format | Article |
| id | doaj.art-55a57bc62ca14fda9bac06f23faea4c5 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T15:09:19Z |
| publishDate | 2015-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-55a57bc62ca14fda9bac06f23faea4c52022-12-22T04:16:42ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2015-08-01481198398910.1590/1414-431x20154590Biological significance of miR-126 expression in atrial fibrillation and heart failureX.J. WeiM. HanF.Y. YangG.C. WeiZ.G. LiangH. YaoC.W. JiR.S. XieC.L. GongY. TianWe investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2−ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those with paroxysmal AF (P<0.05, t-test). Moreover, miR-126 expression was markedly lower in the HF-AF group compared with the AF and HF groups. The 3 patient groups had higher N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, lower left ventricular ejection fraction (LVEF), larger left atrial diameter, and higher cardiothoracic ratio compared with controls. There were significant differences in NT-proBNP levels and LVEF among the AF, HF, and HF-AF groups. Pearson correlation analysis showed that relative miR-126 expression was positively associated with LVEF, logarithm of NT-proBNP, left atrial diameter, cardiothoracic ratio, and age in HF-AF patients. Multiple linear regression analysis showed that miR-126 expression was positively correlated with LVEF, but negatively correlated with the logarithm of NT-pro BNP and the cardiothoracic ratio (all P<0.05). Serum miR-126 levels could serve as a potential candidate biomarker for evaluating the severity of AF and HF. However, to confirm these results, future studies with a larger and diverse patient population are necessary.http://www.scielo.br/pdf/bjmbr/v48n11/1414-431X-bjmbr-1414-431X20154590.pdfMicroRNA-126Atrial fibrillationHeart failureHeart function indicesN-terminal prohormone brain natriuretic peptide (NT-proBNP) |
| spellingShingle | X.J. Wei M. Han F.Y. Yang G.C. Wei Z.G. Liang H. Yao C.W. Ji R.S. Xie C.L. Gong Y. Tian Biological significance of miR-126 expression in atrial fibrillation and heart failure Brazilian Journal of Medical and Biological Research MicroRNA-126 Atrial fibrillation Heart failure Heart function indices N-terminal prohormone brain natriuretic peptide (NT-proBNP) |
| title | Biological significance of miR-126 expression in atrial fibrillation and heart failure |
| title_full | Biological significance of miR-126 expression in atrial fibrillation and heart failure |
| title_fullStr | Biological significance of miR-126 expression in atrial fibrillation and heart failure |
| title_full_unstemmed | Biological significance of miR-126 expression in atrial fibrillation and heart failure |
| title_short | Biological significance of miR-126 expression in atrial fibrillation and heart failure |
| title_sort | biological significance of mir 126 expression in atrial fibrillation and heart failure |
| topic | MicroRNA-126 Atrial fibrillation Heart failure Heart function indices N-terminal prohormone brain natriuretic peptide (NT-proBNP) |
| url | http://www.scielo.br/pdf/bjmbr/v48n11/1414-431X-bjmbr-1414-431X20154590.pdf |
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