Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study
Abstract Background This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. Methods Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 year...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-07-01
|
| Series: | Head & Face Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13005-023-00372-3 |
| _version_ | 1797784353190707200 |
|---|---|
| author | Caio Luiz Bitencourt Reis Mirian Aiko Nakane Matsumoto Flares Baratto-Filho Rafaela Scariot Maria Bernadete Sasso Stuani Fábio Lourenço Romano Ricardo Della Coletta Daniela Silva Barroso de Oliveira Peter Proff Christian Kirschneck Erika Calvano Küchler |
| author_facet | Caio Luiz Bitencourt Reis Mirian Aiko Nakane Matsumoto Flares Baratto-Filho Rafaela Scariot Maria Bernadete Sasso Stuani Fábio Lourenço Romano Ricardo Della Coletta Daniela Silva Barroso de Oliveira Peter Proff Christian Kirschneck Erika Calvano Küchler |
| author_sort | Caio Luiz Bitencourt Reis |
| collection | DOAJ |
| description | Abstract Background This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. Methods Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as “delayed skeletal maturation”, “advanced skeletal maturation” or “normal skeletal maturation”. DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference. Results There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042). Conclusions The rs708111 in the WNT3A gene impacts on skeletal maturation. |
| first_indexed | 2024-03-13T00:39:36Z |
| format | Article |
| id | doaj.art-55a818051cd0400a9876ea30d343c2f3 |
| institution | Directory Open Access Journal |
| issn | 1746-160X |
| language | English |
| last_indexed | 2024-03-13T00:39:36Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Head & Face Medicine |
| spelling | doaj.art-55a818051cd0400a9876ea30d343c2f32023-07-09T11:26:17ZengBMCHead & Face Medicine1746-160X2023-07-011911810.1186/s13005-023-00372-3Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional studyCaio Luiz Bitencourt Reis0Mirian Aiko Nakane Matsumoto1Flares Baratto-Filho2Rafaela Scariot3Maria Bernadete Sasso Stuani4Fábio Lourenço Romano5Ricardo Della Coletta6Daniela Silva Barroso de Oliveira7Peter Proff8Christian Kirschneck9Erika Calvano Küchler10Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São PauloDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São PauloSchool of Dentistry, Tuiuti University of ParanáDepartment of Stomatology, Federal University of ParanáDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São PauloDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São PauloDepartment of Oral Diagnosis, School of Dentistry of Piracicaba, University of Campinas (UNICAMP)Department of Clinic and Surgery, School of Dentistry, Federal University of AlfenasDepartment of Orthodontics, University of RegensburgDepartment of Orthodontics, University of RegensburgDepartment of Orthodontics, University of RegensburgAbstract Background This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. Methods Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as “delayed skeletal maturation”, “advanced skeletal maturation” or “normal skeletal maturation”. DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference. Results There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042). Conclusions The rs708111 in the WNT3A gene impacts on skeletal maturation.https://doi.org/10.1186/s13005-023-00372-3Craniofacial growthm dental developmentSkeletal maturationGenetic variationWNTRUNX2 |
| spellingShingle | Caio Luiz Bitencourt Reis Mirian Aiko Nakane Matsumoto Flares Baratto-Filho Rafaela Scariot Maria Bernadete Sasso Stuani Fábio Lourenço Romano Ricardo Della Coletta Daniela Silva Barroso de Oliveira Peter Proff Christian Kirschneck Erika Calvano Küchler Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study Head & Face Medicine Craniofacial growthm dental development Skeletal maturation Genetic variation WNT RUNX2 |
| title | Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study |
| title_full | Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study |
| title_fullStr | Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study |
| title_full_unstemmed | Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study |
| title_short | Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study |
| title_sort | impact of genetic variations in the wnt family members and runx2 on dental and skeletal maturation a cross sectional study |
| topic | Craniofacial growthm dental development Skeletal maturation Genetic variation WNT RUNX2 |
| url | https://doi.org/10.1186/s13005-023-00372-3 |
| work_keys_str_mv | AT caioluizbitencourtreis impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT mirianaikonakanematsumoto impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT flaresbarattofilho impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT rafaelascariot impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT mariabernadetesassostuani impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT fabiolourencoromano impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT ricardodellacoletta impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT danielasilvabarrosodeoliveira impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT peterproff impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT christiankirschneck impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy AT erikacalvanokuchler impactofgeneticvariationsinthewntfamilymembersandrunx2ondentalandskeletalmaturationacrosssectionalstudy |