Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin

A significant species differences are existed between the skins of the animals according to their lifestyle. The camel is clearly adapted for a desert, and its skin differs in the arrangement and morphology of the hair follicles from that of other domestic mammals. Differential interference Contras...

Full description

Bibliographic Details
Main Authors: Ghasaq S. Mshary, Zainab Y. Kadhim, Karima A. Al Salihi
Format: Article
Language:English
Published: Mirror of Research in Veterinary Sciences and Animals (MRVSA) 2019-05-01
Series:Mirror of Research in Veterinary Sciences and Animals
Subjects:
Online Access:http://mrvsa.com/upload/5%20Application%20of%20differential%20interference%20contrast%20microscpe.pdf
_version_ 1818677454739341312
author Ghasaq S. Mshary
Zainab Y. Kadhim
Karima A. Al Salihi
author_facet Ghasaq S. Mshary
Zainab Y. Kadhim
Karima A. Al Salihi
author_sort Ghasaq S. Mshary
collection DOAJ
description A significant species differences are existed between the skins of the animals according to their lifestyle. The camel is clearly adapted for a desert, and its skin differs in the arrangement and morphology of the hair follicles from that of other domestic mammals. Differential interference Contrast microscope (DICM) is related optics give a specimen a three dimensional appearance that enhance depth of focus so that thicker specimens can be observed at higher magnifications. Consequently, this study was designed to dissect the microanatomy of camelid skin and to investigate the arrangement of its layers and structure with special emphasis on sweat glands by validating a differential interference contrast microscope. Skin sample were collected from different regions of five animals (Camelus dromedaries) that slaughtered at Al-Najaf abattoir/Republic of Iraq. The samples were kept in 10% neutral buffered formalin and processed routinely for histopathological sectioning and examined under DICM. The results of this study revealed a clear structures of all layers and its cells of the camelids skin. Moreover, DICM revealed the distribution and structure of sweat and sebaceous glands together with the organization of hairs and their follicles that vary from other mammals. In conclusion, this study approved the validity of DICM in the examination of camelids skin. The authors recommend to apply this tool in examination of normal skin sections as well as validate this technique in diagnosis of skin diseases.
first_indexed 2024-12-17T08:59:38Z
format Article
id doaj.art-55a8425a957c43f29059e3f018ff9562
institution Directory Open Access Journal
issn 2520-324X
2520-324X
language English
last_indexed 2024-12-17T08:59:38Z
publishDate 2019-05-01
publisher Mirror of Research in Veterinary Sciences and Animals (MRVSA)
record_format Article
series Mirror of Research in Veterinary Sciences and Animals
spelling doaj.art-55a8425a957c43f29059e3f018ff95622022-12-21T21:55:49ZengMirror of Research in Veterinary Sciences and Animals (MRVSA)Mirror of Research in Veterinary Sciences and Animals2520-324X2520-324X2019-05-01723442Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skinGhasaq S. Mshary 0Zainab Y. Kadhim 1Karima A. Al Salihi2College of Veterinary Medicine/ AL- Muthanna University/ IraqCollege of Veterinary Medicine/ AL- Muthanna University/ IraqCollege of Veterinary Medicine/ AL- Muthanna University/ IraqA significant species differences are existed between the skins of the animals according to their lifestyle. The camel is clearly adapted for a desert, and its skin differs in the arrangement and morphology of the hair follicles from that of other domestic mammals. Differential interference Contrast microscope (DICM) is related optics give a specimen a three dimensional appearance that enhance depth of focus so that thicker specimens can be observed at higher magnifications. Consequently, this study was designed to dissect the microanatomy of camelid skin and to investigate the arrangement of its layers and structure with special emphasis on sweat glands by validating a differential interference contrast microscope. Skin sample were collected from different regions of five animals (Camelus dromedaries) that slaughtered at Al-Najaf abattoir/Republic of Iraq. The samples were kept in 10% neutral buffered formalin and processed routinely for histopathological sectioning and examined under DICM. The results of this study revealed a clear structures of all layers and its cells of the camelids skin. Moreover, DICM revealed the distribution and structure of sweat and sebaceous glands together with the organization of hairs and their follicles that vary from other mammals. In conclusion, this study approved the validity of DICM in the examination of camelids skin. The authors recommend to apply this tool in examination of normal skin sections as well as validate this technique in diagnosis of skin diseases.http://mrvsa.com/upload/5%20Application%20of%20differential%20interference%20contrast%20microscpe.pdfCamelidsDICMSkinsweat glandssebaceous glands.
spellingShingle Ghasaq S. Mshary
Zainab Y. Kadhim
Karima A. Al Salihi
Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
Mirror of Research in Veterinary Sciences and Animals
Camelids
DICM
Skin
sweat glands
sebaceous glands.
title Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
title_full Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
title_fullStr Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
title_full_unstemmed Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
title_short Application of Differential Interference Contrast microscope in studying of Camelus dromedaries skin
title_sort application of differential interference contrast microscope in studying of camelus dromedaries skin
topic Camelids
DICM
Skin
sweat glands
sebaceous glands.
url http://mrvsa.com/upload/5%20Application%20of%20differential%20interference%20contrast%20microscpe.pdf
work_keys_str_mv AT ghasaqsmshary applicationofdifferentialinterferencecontrastmicroscopeinstudyingofcamelusdromedariesskin
AT zainabykadhim applicationofdifferentialinterferencecontrastmicroscopeinstudyingofcamelusdromedariesskin
AT karimaaalsalihi applicationofdifferentialinterferencecontrastmicroscopeinstudyingofcamelusdromedariesskin