<i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress
As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF) may be effective in enhancing plant resilience to drought, one of the major limiting factors threatening crop productivity. AMF host their own microbiota and previous data demonstrated that endobacteria thriving in <i>Gigas...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/7/886 |
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| author | Matteo Chialva Luisa Lanfranco Gianluca Guazzotti Veronica Santoro Mara Novero Paola Bonfante |
| author_facet | Matteo Chialva Luisa Lanfranco Gianluca Guazzotti Veronica Santoro Mara Novero Paola Bonfante |
| author_sort | Matteo Chialva |
| collection | DOAJ |
| description | As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF) may be effective in enhancing plant resilience to drought, one of the major limiting factors threatening crop productivity. AMF host their own microbiota and previous data demonstrated that endobacteria thriving in <i>Gigaspora margarita</i> modulate fungal antioxidant responses. Here, we used the <i>G. margarita</i>–<i>Candidatus</i> Glomeribacter gigasporarum system to test whether the tripartite interaction between tomato, <i>G. margarita</i> and its endobacteria may improve plant resilience to combined water/nutrient stress. Tomato plants were inoculated with spores containing endobacteria (B+) or not (B-), and exposed to combined water/nutrient stress. Plants traits, AM colonization and expression of AM marker genes were measured. Results showed that mycorrhizal frequency was low and no growth effect was observed. Under control conditions, B+ inoculated plants were more responsive to the symbiosis, as they showed an up-regulation of three AM marker genes involved in phosphate and lipids metabolism compared with B− inoculated or not-inoculated plants. When combined stress was imposed, the difference between fungal strains was still evident for one marker gene. These results indicate that the fungal endobacteria finely modulate plant metabolism, even in the absence of growth response. |
| first_indexed | 2024-03-10T18:29:35Z |
| format | Article |
| id | doaj.art-55b27a2004074340a50c227d7dd33f61 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:29:35Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-55b27a2004074340a50c227d7dd33f612023-11-20T06:41:42ZengMDPI AGPlants2223-77472020-07-019788610.3390/plants9070886<i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient StressMatteo Chialva0Luisa Lanfranco1Gianluca Guazzotti2Veronica Santoro3Mara Novero4Paola Bonfante5Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, ItalyDepartment of Agricultural, Forest and Food Science, University of Torino, Largo Braccini 2, I-10095 Grugliasco, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, ItalyDepartment of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, ItalyAs members of the plant microbiota, arbuscular mycorrhizal fungi (AMF) may be effective in enhancing plant resilience to drought, one of the major limiting factors threatening crop productivity. AMF host their own microbiota and previous data demonstrated that endobacteria thriving in <i>Gigaspora margarita</i> modulate fungal antioxidant responses. Here, we used the <i>G. margarita</i>–<i>Candidatus</i> Glomeribacter gigasporarum system to test whether the tripartite interaction between tomato, <i>G. margarita</i> and its endobacteria may improve plant resilience to combined water/nutrient stress. Tomato plants were inoculated with spores containing endobacteria (B+) or not (B-), and exposed to combined water/nutrient stress. Plants traits, AM colonization and expression of AM marker genes were measured. Results showed that mycorrhizal frequency was low and no growth effect was observed. Under control conditions, B+ inoculated plants were more responsive to the symbiosis, as they showed an up-regulation of three AM marker genes involved in phosphate and lipids metabolism compared with B− inoculated or not-inoculated plants. When combined stress was imposed, the difference between fungal strains was still evident for one marker gene. These results indicate that the fungal endobacteria finely modulate plant metabolism, even in the absence of growth response.https://www.mdpi.com/2223-7747/9/7/886arbuscular mycorrhizal fungidroughtendobacteriamultiple stressnutrientsstress resilience |
| spellingShingle | Matteo Chialva Luisa Lanfranco Gianluca Guazzotti Veronica Santoro Mara Novero Paola Bonfante <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress Plants arbuscular mycorrhizal fungi drought endobacteria multiple stress nutrients stress resilience |
| title | <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress |
| title_full | <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress |
| title_fullStr | <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress |
| title_full_unstemmed | <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress |
| title_short | <i>Gigaspora margarita</i> and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress |
| title_sort | i gigaspora margarita i and its endobacterium modulate symbiotic marker genes in tomato roots under combined water and nutrient stress |
| topic | arbuscular mycorrhizal fungi drought endobacteria multiple stress nutrients stress resilience |
| url | https://www.mdpi.com/2223-7747/9/7/886 |
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