The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway

Breast cancer is the most common female malignancy worldwide, however its molecular pathogenesis still needs in-depth investigation. Here we first revealed that the olfactory receptor family 2, subfamily T, member 6 (OR2T6) was significantly over-expressed in breast cancer tissues compared with norm...

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Main Authors: Ming Li, Xiao Wang, Ran-Ran Ma, Duan-Bo Shi, Ya-Wen Wang, Xiao-Mei Li, Jun-Yi He, Jun Wang, Peng Gao
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Oncology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fonc.2019.01210/full
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author Ming Li
Ming Li
Xiao Wang
Xiao Wang
Ran-Ran Ma
Ran-Ran Ma
Duan-Bo Shi
Duan-Bo Shi
Ya-Wen Wang
Xiao-Mei Li
Jun-Yi He
Jun-Yi He
Jun Wang
Peng Gao
Peng Gao
author_facet Ming Li
Ming Li
Xiao Wang
Xiao Wang
Ran-Ran Ma
Ran-Ran Ma
Duan-Bo Shi
Duan-Bo Shi
Ya-Wen Wang
Xiao-Mei Li
Jun-Yi He
Jun-Yi He
Jun Wang
Peng Gao
Peng Gao
author_sort Ming Li
collection DOAJ
description Breast cancer is the most common female malignancy worldwide, however its molecular pathogenesis still needs in-depth investigation. Here we first revealed that the olfactory receptor family 2, subfamily T, member 6 (OR2T6) was significantly over-expressed in breast cancer tissues compared with normal breast tissues. OR2T6 expression was tightly correlated with higher TNM staging, positive lymph node metastasis, and associated with poorer patients' overall and disease-free survival. And OR2T6 enhanced the proliferation, invasion, and migration ability of breast cancer cell lines in vitro (MCF-7 and MDA-MD-231). Mechanically, it promoted the expression of mesenchymal markers (Vimentin, N-cadherin, and β-catenin) while inhibited E-cadherin expression, suggesting that OR2T6 played a key role in the regulation of epithelial-mesenchymal transition (EMT) process. Moreover, the human gene expression microarray clarified that MAPK/ERK pathway could be initiated by OR2T6 at mRNA level, which was further confirmed at protein level by western blot analysis. Thus, we concluded that OR2T6, as a novel oncogene, contributed to the progression of breast carcinoma by the initiation of EMT and MAPK/ERK pathway.
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spelling doaj.art-55b45ddc57514ca580785997dfdc22ee2022-12-21T19:49:03ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-11-01910.3389/fonc.2019.01210493939The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK PathwayMing Li0Ming Li1Xiao Wang2Xiao Wang3Ran-Ran Ma4Ran-Ran Ma5Duan-Bo Shi6Duan-Bo Shi7Ya-Wen Wang8Xiao-Mei Li9Jun-Yi He10Jun-Yi He11Jun Wang12Peng Gao13Peng Gao14Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Dezhou People's Hospital, Dezhou, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Qilu Hospital, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Qilu Hospital, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Qilu Hospital, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Qilu Hospital, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaKey Laboratory for Experimental Teratology of the Ministry of Education and Department of Pathology, School of Medicine, Shandong University, Jinan, ChinaDepartment of Pathology, Qilu Hospital, Shandong University, Jinan, ChinaBreast cancer is the most common female malignancy worldwide, however its molecular pathogenesis still needs in-depth investigation. Here we first revealed that the olfactory receptor family 2, subfamily T, member 6 (OR2T6) was significantly over-expressed in breast cancer tissues compared with normal breast tissues. OR2T6 expression was tightly correlated with higher TNM staging, positive lymph node metastasis, and associated with poorer patients' overall and disease-free survival. And OR2T6 enhanced the proliferation, invasion, and migration ability of breast cancer cell lines in vitro (MCF-7 and MDA-MD-231). Mechanically, it promoted the expression of mesenchymal markers (Vimentin, N-cadherin, and β-catenin) while inhibited E-cadherin expression, suggesting that OR2T6 played a key role in the regulation of epithelial-mesenchymal transition (EMT) process. Moreover, the human gene expression microarray clarified that MAPK/ERK pathway could be initiated by OR2T6 at mRNA level, which was further confirmed at protein level by western blot analysis. Thus, we concluded that OR2T6, as a novel oncogene, contributed to the progression of breast carcinoma by the initiation of EMT and MAPK/ERK pathway.https://www.frontiersin.org/article/10.3389/fonc.2019.01210/fullOR2T6breast carcinomaepithelial-mesenchymal transitionMAPK/ERK pathwayprognosis
spellingShingle Ming Li
Ming Li
Xiao Wang
Xiao Wang
Ran-Ran Ma
Ran-Ran Ma
Duan-Bo Shi
Duan-Bo Shi
Ya-Wen Wang
Xiao-Mei Li
Jun-Yi He
Jun-Yi He
Jun Wang
Peng Gao
Peng Gao
The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
Frontiers in Oncology
OR2T6
breast carcinoma
epithelial-mesenchymal transition
MAPK/ERK pathway
prognosis
title The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
title_full The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
title_fullStr The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
title_full_unstemmed The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
title_short The Olfactory Receptor Family 2, Subfamily T, Member 6 (OR2T6) Is Involved in Breast Cancer Progression via Initiating Epithelial-Mesenchymal Transition and MAPK/ERK Pathway
title_sort olfactory receptor family 2 subfamily t member 6 or2t6 is involved in breast cancer progression via initiating epithelial mesenchymal transition and mapk erk pathway
topic OR2T6
breast carcinoma
epithelial-mesenchymal transition
MAPK/ERK pathway
prognosis
url https://www.frontiersin.org/article/10.3389/fonc.2019.01210/full
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