Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats
The detrimental effect of diclofenac sodium (Diclo-Na) on male reproductive organs is reported upon in this paper. Chitosan is a polysaccharide composed of various amounts of glucosamine. Chitosan nanoparticles (CH-NPs) have attracted much attention owing to their biomedical activity. Selenium (Se)...
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MDPI AG
2021-11-01
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| author | Samy M. El-Megharbel Fawziah A. Al-Salmi Sarah Al-Harthi Khadeejah Alsolami Reham Z. Hamza |
| author_facet | Samy M. El-Megharbel Fawziah A. Al-Salmi Sarah Al-Harthi Khadeejah Alsolami Reham Z. Hamza |
| author_sort | Samy M. El-Megharbel |
| collection | DOAJ |
| description | The detrimental effect of diclofenac sodium (Diclo-Na) on male reproductive organs is reported upon in this paper. Chitosan is a polysaccharide composed of various amounts of glucosamine. Chitosan nanoparticles (CH-NPs) have attracted much attention owing to their biomedical activity. Selenium (Se) has a vital role in nutrition, plays an important role in enhancing male reproduction, and has a wide range of free radical scavenging activities. However, the study of the impact of chitosan nanoparticles in combination with Se (IV) (CH-NPs/Se) on male reproductive toxicity associated with Diclo-Na administration is lacking in recent literature. The current study assessed the ameliorative effects of complexes of CH-NPs/Se (IV) on Diclo-Na and the ways in which they alter reproductive toxicity in male rats. Male rats were treated for 30 days successively, either with Diclo-Na (10 mg/kg) or co-treated with a CH-NPs/Se complex (280 mg/kg). Sperm characteristics, marker enzymes of testicular function, LH, FSH, and testosterone were evaluated in addition to oxidative stress markers and histological alterations. CH-NPs/Se significantly alleviated Diclo-Na-induced decline in sperm count and motility, testicular function enzymes, and levels of LH and testosterone in serum. Additionally, CH-NPs/Se co-administration at 280 mg/Kg, inhibited the Diclo-Na-induced decline of antioxidant enzyme activities and elevated oxidative stress indices and reactive free radicals in testicular homogenates of male rats. CH-NPs/Se (280 mg/kg) alone improved Diclo-Na and ameliorated histological damages in exposed rats. In conclusion, chitosan improved testicular function in Diclo-Na-treated rats by enhancing the testosterone hormone levels, ameliorating testicular tissue, and inhibiting markers of oxidative stress in male rats. |
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| last_indexed | 2024-03-10T04:21:49Z |
| publishDate | 2021-11-01 |
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| series | Crystals |
| spelling | doaj.art-55b519ff5d8f4854bcd6f07d6dbc91f82023-11-23T07:48:16ZengMDPI AGCrystals2073-43522021-11-011112147710.3390/cryst11121477Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male RatsSamy M. El-Megharbel0Fawziah A. Al-Salmi1Sarah Al-Harthi2Khadeejah Alsolami3Reham Z. Hamza4Chemistry Department, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBiology Department, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaChemistry Department, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaPharmacology and Toxicology Department, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBiology Department, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaThe detrimental effect of diclofenac sodium (Diclo-Na) on male reproductive organs is reported upon in this paper. Chitosan is a polysaccharide composed of various amounts of glucosamine. Chitosan nanoparticles (CH-NPs) have attracted much attention owing to their biomedical activity. Selenium (Se) has a vital role in nutrition, plays an important role in enhancing male reproduction, and has a wide range of free radical scavenging activities. However, the study of the impact of chitosan nanoparticles in combination with Se (IV) (CH-NPs/Se) on male reproductive toxicity associated with Diclo-Na administration is lacking in recent literature. The current study assessed the ameliorative effects of complexes of CH-NPs/Se (IV) on Diclo-Na and the ways in which they alter reproductive toxicity in male rats. Male rats were treated for 30 days successively, either with Diclo-Na (10 mg/kg) or co-treated with a CH-NPs/Se complex (280 mg/kg). Sperm characteristics, marker enzymes of testicular function, LH, FSH, and testosterone were evaluated in addition to oxidative stress markers and histological alterations. CH-NPs/Se significantly alleviated Diclo-Na-induced decline in sperm count and motility, testicular function enzymes, and levels of LH and testosterone in serum. Additionally, CH-NPs/Se co-administration at 280 mg/Kg, inhibited the Diclo-Na-induced decline of antioxidant enzyme activities and elevated oxidative stress indices and reactive free radicals in testicular homogenates of male rats. CH-NPs/Se (280 mg/kg) alone improved Diclo-Na and ameliorated histological damages in exposed rats. In conclusion, chitosan improved testicular function in Diclo-Na-treated rats by enhancing the testosterone hormone levels, ameliorating testicular tissue, and inhibiting markers of oxidative stress in male rats.https://www.mdpi.com/2073-4352/11/12/1477male reproductionseleniumdiclofenac-sodiumoxidative stress markerschitosan nanoparticles |
| spellingShingle | Samy M. El-Megharbel Fawziah A. Al-Salmi Sarah Al-Harthi Khadeejah Alsolami Reham Z. Hamza Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats Crystals male reproduction selenium diclofenac-sodium oxidative stress markers chitosan nanoparticles |
| title | Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats |
| title_full | Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats |
| title_fullStr | Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats |
| title_full_unstemmed | Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats |
| title_short | Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats |
| title_sort | chitosan selenium nanoparticles attenuate diclofenac sodium induced testicular toxicity in male rats |
| topic | male reproduction selenium diclofenac-sodium oxidative stress markers chitosan nanoparticles |
| url | https://www.mdpi.com/2073-4352/11/12/1477 |
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