Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication.
Break-induced replication (BIR) is a highly mutagenic eukaryotic homologous DNA recombination pathway that repairs one-ended DNA double strand breaks such as broken DNA replication forks and eroded telomeres. While searching for cis-acting factors regulating ectopic BIR efficiency, we found that ect...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-06-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010124 |
| _version_ | 1811294859340808192 |
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| author | Tannia Uribe-Calvillo Laetitia Maestroni Marie-Claude Marsolier Basheer Khadaroo Christine Arbiol Jonathan Schott Bertrand Llorente |
| author_facet | Tannia Uribe-Calvillo Laetitia Maestroni Marie-Claude Marsolier Basheer Khadaroo Christine Arbiol Jonathan Schott Bertrand Llorente |
| author_sort | Tannia Uribe-Calvillo |
| collection | DOAJ |
| description | Break-induced replication (BIR) is a highly mutagenic eukaryotic homologous DNA recombination pathway that repairs one-ended DNA double strand breaks such as broken DNA replication forks and eroded telomeres. While searching for cis-acting factors regulating ectopic BIR efficiency, we found that ectopic BIR efficiency is the highest close to chromosome ends. The variations of ectopic BIR efficiency as a function of the length of DNA to replicate can be described as a combination of two decreasing exponential functions, a property in line with repeated cycles of strand invasion, elongation and dissociation that characterize BIR. Interestingly, the apparent processivity of ectopic BIR depends on the length of DNA already synthesized. Ectopic BIR is more susceptible to disruption during the synthesis of the first ~35-40 kb of DNA than later, notably when the template chromatid is being transcribed or heterochromatic. Finally, we show that the Srs2 helicase promotes ectopic BIR from both telomere proximal and telomere distal regions in diploid cells but only from telomere proximal sites in haploid cells. Altogether, we bring new light on the factors impacting a last resort DNA repair pathway. |
| first_indexed | 2024-04-13T05:23:53Z |
| format | Article |
| id | doaj.art-55b6cab540ea429eac55b374be07d434 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T05:23:53Z |
| publishDate | 2022-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-55b6cab540ea429eac55b374be07d4342022-12-22T03:00:39ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-06-01186e101012410.1371/journal.pgen.1010124Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication.Tannia Uribe-CalvilloLaetitia MaestroniMarie-Claude MarsolierBasheer KhadarooChristine ArbiolJonathan SchottBertrand LlorenteBreak-induced replication (BIR) is a highly mutagenic eukaryotic homologous DNA recombination pathway that repairs one-ended DNA double strand breaks such as broken DNA replication forks and eroded telomeres. While searching for cis-acting factors regulating ectopic BIR efficiency, we found that ectopic BIR efficiency is the highest close to chromosome ends. The variations of ectopic BIR efficiency as a function of the length of DNA to replicate can be described as a combination of two decreasing exponential functions, a property in line with repeated cycles of strand invasion, elongation and dissociation that characterize BIR. Interestingly, the apparent processivity of ectopic BIR depends on the length of DNA already synthesized. Ectopic BIR is more susceptible to disruption during the synthesis of the first ~35-40 kb of DNA than later, notably when the template chromatid is being transcribed or heterochromatic. Finally, we show that the Srs2 helicase promotes ectopic BIR from both telomere proximal and telomere distal regions in diploid cells but only from telomere proximal sites in haploid cells. Altogether, we bring new light on the factors impacting a last resort DNA repair pathway.https://doi.org/10.1371/journal.pgen.1010124 |
| spellingShingle | Tannia Uribe-Calvillo Laetitia Maestroni Marie-Claude Marsolier Basheer Khadaroo Christine Arbiol Jonathan Schott Bertrand Llorente Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. PLoS Genetics |
| title | Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. |
| title_full | Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. |
| title_fullStr | Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. |
| title_full_unstemmed | Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. |
| title_short | Comprehensive analysis of cis- and trans-acting factors affecting ectopic Break-Induced Replication. |
| title_sort | comprehensive analysis of cis and trans acting factors affecting ectopic break induced replication |
| url | https://doi.org/10.1371/journal.pgen.1010124 |
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