Diapause induces functional axonal regeneration after necrotic insult in C. elegans.
Many neurons are unable to regenerate after damage. The ability to regenerate after an insult depends on life stage, neuronal subtype, intrinsic and extrinsic factors. C. elegans is a powerful model to test the genetic and environmental factors that affect axonal regeneration after damage, since its...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1007863 |
| _version_ | 1827919222991224832 |
|---|---|
| author | Mauricio Caneo Victoria Julian Alexandra B Byrne Mark J Alkema Andrea Calixto |
| author_facet | Mauricio Caneo Victoria Julian Alexandra B Byrne Mark J Alkema Andrea Calixto |
| author_sort | Mauricio Caneo |
| collection | DOAJ |
| description | Many neurons are unable to regenerate after damage. The ability to regenerate after an insult depends on life stage, neuronal subtype, intrinsic and extrinsic factors. C. elegans is a powerful model to test the genetic and environmental factors that affect axonal regeneration after damage, since its axons can regenerate after neuronal insult. Here we demonstrate that diapause promotes the complete morphological regeneration of truncated touch receptor neuron (TRN) axons expressing a neurotoxic MEC-4(d) DEG/ENaC channel. Truncated axons of different lengths were repaired during diapause and we observed potent axonal regrowth from somas alone. Complete morphological regeneration depends on DLK-1 but neuronal sprouting and outgrowth is DLK-1 independent. We show that TRN regeneration is fully functional since animals regain their ability to respond to mechanical stimulation. Thus, diapause induced regeneration provides a simple model of complete axonal regeneration which will greatly facilitate the study of environmental and genetic factors affecting the rate at which neurons die. |
| first_indexed | 2024-03-13T03:55:47Z |
| format | Article |
| id | doaj.art-55bcd6bf47b045f8a8d5ae4afffe8d62 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-03-13T03:55:47Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-55bcd6bf47b045f8a8d5ae4afffe8d622023-06-22T05:31:30ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-01-01151e100786310.1371/journal.pgen.1007863Diapause induces functional axonal regeneration after necrotic insult in C. elegans.Mauricio CaneoVictoria JulianAlexandra B ByrneMark J AlkemaAndrea CalixtoMany neurons are unable to regenerate after damage. The ability to regenerate after an insult depends on life stage, neuronal subtype, intrinsic and extrinsic factors. C. elegans is a powerful model to test the genetic and environmental factors that affect axonal regeneration after damage, since its axons can regenerate after neuronal insult. Here we demonstrate that diapause promotes the complete morphological regeneration of truncated touch receptor neuron (TRN) axons expressing a neurotoxic MEC-4(d) DEG/ENaC channel. Truncated axons of different lengths were repaired during diapause and we observed potent axonal regrowth from somas alone. Complete morphological regeneration depends on DLK-1 but neuronal sprouting and outgrowth is DLK-1 independent. We show that TRN regeneration is fully functional since animals regain their ability to respond to mechanical stimulation. Thus, diapause induced regeneration provides a simple model of complete axonal regeneration which will greatly facilitate the study of environmental and genetic factors affecting the rate at which neurons die.https://doi.org/10.1371/journal.pgen.1007863 |
| spellingShingle | Mauricio Caneo Victoria Julian Alexandra B Byrne Mark J Alkema Andrea Calixto Diapause induces functional axonal regeneration after necrotic insult in C. elegans. PLoS Genetics |
| title | Diapause induces functional axonal regeneration after necrotic insult in C. elegans. |
| title_full | Diapause induces functional axonal regeneration after necrotic insult in C. elegans. |
| title_fullStr | Diapause induces functional axonal regeneration after necrotic insult in C. elegans. |
| title_full_unstemmed | Diapause induces functional axonal regeneration after necrotic insult in C. elegans. |
| title_short | Diapause induces functional axonal regeneration after necrotic insult in C. elegans. |
| title_sort | diapause induces functional axonal regeneration after necrotic insult in c elegans |
| url | https://doi.org/10.1371/journal.pgen.1007863 |
| work_keys_str_mv | AT mauriciocaneo diapauseinducesfunctionalaxonalregenerationafternecroticinsultincelegans AT victoriajulian diapauseinducesfunctionalaxonalregenerationafternecroticinsultincelegans AT alexandrabbyrne diapauseinducesfunctionalaxonalregenerationafternecroticinsultincelegans AT markjalkema diapauseinducesfunctionalaxonalregenerationafternecroticinsultincelegans AT andreacalixto diapauseinducesfunctionalaxonalregenerationafternecroticinsultincelegans |