Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection
The interest in sustainable and low-cost materials for the production of new sensing platforms has been increasing over the time. The possibility of obtaining reliable sensors, on a large scale, easy-to-produce, and cost-effective allows for the development of analytical devices with adequate charac...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053922000455 |
| _version_ | 1811187703075569664 |
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| author | Rodrigo Vieira Blasques Jéssica S. Stefano Jéssica R. Camargo Luiz R. Guterres e Silva Laís Canniatti Brazaca Bruno Campos Janegitz |
| author_facet | Rodrigo Vieira Blasques Jéssica S. Stefano Jéssica R. Camargo Luiz R. Guterres e Silva Laís Canniatti Brazaca Bruno Campos Janegitz |
| author_sort | Rodrigo Vieira Blasques |
| collection | DOAJ |
| description | The interest in sustainable and low-cost materials for the production of new sensing platforms has been increasing over the time. The possibility of obtaining reliable sensors, on a large scale, easy-to-produce, and cost-effective allows for the development of analytical devices with adequate characteristics for point-of-care analysis. In this context, we present the development of a new low-cost and easy-to-produce disposable electrochemical device composed of a conductive ink modified with Prussian blue for the detection of uric acid, hydrogen peroxide, and glucose. The electrode surface was characterized by scanning electron microscopy coupled with energy dispersive spectroscopy, Fourier-transform infrared spectroscopy, and cyclic voltammetric techniques. Also, the proposed sensors and biosensors were applied for the electrochemical determination of uric acid, hydrogen peroxide, and glucose. The device achieved a linear response ranging from 5.0 to 150.0 μmol L−1 for uric acid, with a limit of detection of 0.70 μmol L−1 using differential pulse voltammetry. The detection of hydrogen peroxide and glucose was performed using amperometry at a working potential of −0.3 V, which. Hydrogen peroxide sensing presented a linear range varying from 100.0 to 800.0 µmol L−1, with a LOD of 31.6 μmol L−1. A glucose biosensor was developed after modification of the electrode with a film composed of chitosan, glutaraldehyde, and glucose oxidase, which presented a linear range varying from 50.0 to 500.0 μmol L−1, with a LOD of 9.20 μmol L−1. Also, the construction of a 3D printed wearable wristband was proposed facing uric acid determination, to show the versatility of the sensor. |
| first_indexed | 2024-04-11T14:07:56Z |
| format | Article |
| id | doaj.art-55bfd14461ff43a68b16ed247634662f |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-04-11T14:07:56Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-55bfd14461ff43a68b16ed247634662f2022-12-22T04:19:49ZengElsevierSensors and Actuators Reports2666-05392022-11-014100118Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detectionRodrigo Vieira Blasques0Jéssica S. Stefano1Jéssica R. Camargo2Luiz R. Guterres e Silva3Laís Canniatti Brazaca4Bruno Campos Janegitz5Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil; Department of Physics, Chemistry, and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo 18052-780, BrazilLaboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, BrazilLaboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil; Department of Physics, Chemistry, and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo 18052-780, BrazilLaboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil; Department of Physics, Chemistry, and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo 18052-780, BrazilDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, United StatesLaboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil; Corresponding author.The interest in sustainable and low-cost materials for the production of new sensing platforms has been increasing over the time. The possibility of obtaining reliable sensors, on a large scale, easy-to-produce, and cost-effective allows for the development of analytical devices with adequate characteristics for point-of-care analysis. In this context, we present the development of a new low-cost and easy-to-produce disposable electrochemical device composed of a conductive ink modified with Prussian blue for the detection of uric acid, hydrogen peroxide, and glucose. The electrode surface was characterized by scanning electron microscopy coupled with energy dispersive spectroscopy, Fourier-transform infrared spectroscopy, and cyclic voltammetric techniques. Also, the proposed sensors and biosensors were applied for the electrochemical determination of uric acid, hydrogen peroxide, and glucose. The device achieved a linear response ranging from 5.0 to 150.0 μmol L−1 for uric acid, with a limit of detection of 0.70 μmol L−1 using differential pulse voltammetry. The detection of hydrogen peroxide and glucose was performed using amperometry at a working potential of −0.3 V, which. Hydrogen peroxide sensing presented a linear range varying from 100.0 to 800.0 µmol L−1, with a LOD of 31.6 μmol L−1. A glucose biosensor was developed after modification of the electrode with a film composed of chitosan, glutaraldehyde, and glucose oxidase, which presented a linear range varying from 50.0 to 500.0 μmol L−1, with a LOD of 9.20 μmol L−1. Also, the construction of a 3D printed wearable wristband was proposed facing uric acid determination, to show the versatility of the sensor.http://www.sciencedirect.com/science/article/pii/S2666053922000455Screen printed electrodeLab-made conductive inkPoint-of-carePrussian blue modified electrodeGlucose biosensing |
| spellingShingle | Rodrigo Vieira Blasques Jéssica S. Stefano Jéssica R. Camargo Luiz R. Guterres e Silva Laís Canniatti Brazaca Bruno Campos Janegitz Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection Sensors and Actuators Reports Screen printed electrode Lab-made conductive ink Point-of-care Prussian blue modified electrode Glucose biosensing |
| title | Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection |
| title_full | Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection |
| title_fullStr | Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection |
| title_full_unstemmed | Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection |
| title_short | Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection |
| title_sort | disposable prussian blue anchored electrochemical sensor for enzymatic and non enzymatic multi analyte detection |
| topic | Screen printed electrode Lab-made conductive ink Point-of-care Prussian blue modified electrode Glucose biosensing |
| url | http://www.sciencedirect.com/science/article/pii/S2666053922000455 |
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