Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media
The paper presents results of quantitative and qualitative analyses of the composition of saprophytic and pathogenic microflora colonising contemporary wood of Scots pine (Pinus sylvestris L.) and oak (Quercus sp.), deposited for eight years in peat soil, under anoxia conditions at two measuring sta...
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| Format: | Article |
| Language: | English |
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Wydawnictwo Naukowe Uniwersytetu Kardynała Stefana Wyszyńskiego
2021-10-01
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| Series: | Studia Ecologiae et Bioethicae |
| Subjects: | |
| Online Access: | https://czasopisma.uksw.edu.pl/index.php/seb/article/view/9151 |
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| author | Zbigniew Wyżewski Julitta Gajewska Hanna Rekosz-Burlaga Leszek Babiński Bogusława Waliszewska Monika Sitarek-Andrzejczyk |
| author_facet | Zbigniew Wyżewski Julitta Gajewska Hanna Rekosz-Burlaga Leszek Babiński Bogusława Waliszewska Monika Sitarek-Andrzejczyk |
| author_sort | Zbigniew Wyżewski |
| collection | DOAJ |
| description | The paper presents results of quantitative and qualitative analyses of the composition of saprophytic and pathogenic microflora colonising contemporary wood of Scots pine (Pinus sylvestris L.) and oak (Quercus sp.), deposited for eight years in peat soil, under anoxia conditions at two measuring stations located at the fortified settlement of the Lusatian culture in Biskupin. Qualitative determinations of bacteria were performed based on the analyses of their cultural, morphological, physiological, and bio-chemical characteristics. Petrifilms™ and conventional bacteriological media were used for quantitative analyses. Bacteria in the examined wood and surrounding soil were identified as belonging to species, including those of the genera Pseudomonas, Clostridium (Cl. butyricum/beierinckii, Cl. perfringens), Bacillus, Corynebacterium, bacteria from the family Enterobacteriaceae, as well as aerobic and anaerobic cellulolytic bacteria. An important correlation between the standard pour plate method and PetrifilmTM was observed. Petrifilms are an effective alternative, in comparison with traditional methods, for the determination of total bacterial counts for wood samples. |
| first_indexed | 2024-04-12T02:55:08Z |
| format | Article |
| id | doaj.art-55c24667a792412393d677f97d01290d |
| institution | Directory Open Access Journal |
| issn | 1733-1218 |
| language | English |
| last_indexed | 2024-04-12T02:55:08Z |
| publishDate | 2021-10-01 |
| publisher | Wydawnictwo Naukowe Uniwersytetu Kardynała Stefana Wyszyńskiego |
| record_format | Article |
| series | Studia Ecologiae et Bioethicae |
| spelling | doaj.art-55c24667a792412393d677f97d01290d2022-12-22T03:50:51ZengWydawnictwo Naukowe Uniwersytetu Kardynała Stefana WyszyńskiegoStudia Ecologiae et Bioethicae1733-12182021-10-0119310.21697/seb.2021.19.3.07Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological MediaZbigniew Wyżewski0Julitta Gajewska1Hanna Rekosz-Burlaga2Leszek Babiński3Bogusława Waliszewska4Monika Sitarek-Andrzejczyk5Institute of Biological Sciences, Cardinal Stefan Wyszyński University in Warsaw,Institute of Agriculture and Biology, Warsaw University of Life Sciences, Institute of Agriculture and Biology, Warsaw University of Life Sciences, Department of Conservation, Archaeological Museum in Biskupin, Institute of Chemical Wood Technology, Poznań University of Life Sciences, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, The paper presents results of quantitative and qualitative analyses of the composition of saprophytic and pathogenic microflora colonising contemporary wood of Scots pine (Pinus sylvestris L.) and oak (Quercus sp.), deposited for eight years in peat soil, under anoxia conditions at two measuring stations located at the fortified settlement of the Lusatian culture in Biskupin. Qualitative determinations of bacteria were performed based on the analyses of their cultural, morphological, physiological, and bio-chemical characteristics. Petrifilms™ and conventional bacteriological media were used for quantitative analyses. Bacteria in the examined wood and surrounding soil were identified as belonging to species, including those of the genera Pseudomonas, Clostridium (Cl. butyricum/beierinckii, Cl. perfringens), Bacillus, Corynebacterium, bacteria from the family Enterobacteriaceae, as well as aerobic and anaerobic cellulolytic bacteria. An important correlation between the standard pour plate method and PetrifilmTM was observed. Petrifilms are an effective alternative, in comparison with traditional methods, for the determination of total bacterial counts for wood samples.https://czasopisma.uksw.edu.pl/index.php/seb/article/view/9151wooddegradationarcheological sitebacteriapour plate methodPetrifilm™ |
| spellingShingle | Zbigniew Wyżewski Julitta Gajewska Hanna Rekosz-Burlaga Leszek Babiński Bogusława Waliszewska Monika Sitarek-Andrzejczyk Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media Studia Ecologiae et Bioethicae wood degradation archeological site bacteria pour plate method Petrifilm™ |
| title | Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media |
| title_full | Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media |
| title_fullStr | Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media |
| title_full_unstemmed | Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media |
| title_short | Bacteriological Characteristics of Contemporary Wood During Soil Deposition in Anoxia Conditions at the Biskupin Archaeological Site (Poland) - Petrifilms™ as an Alternative for Conventional Bacteriological Media |
| title_sort | bacteriological characteristics of contemporary wood during soil deposition in anoxia conditions at the biskupin archaeological site poland petrifilms™ as an alternative for conventional bacteriological media |
| topic | wood degradation archeological site bacteria pour plate method Petrifilm™ |
| url | https://czasopisma.uksw.edu.pl/index.php/seb/article/view/9151 |
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