Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet
The use of translationally relevant animal models is essential, also within the field of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Compared to frequently used mouse and rat models, the hamster may provide a higher degree of physiological similarity to humans i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/13/2/604 |
| _version_ | 1797396781942702080 |
|---|---|
| author | Victoria Svop Jensen Christian Fledelius Erik Max Wulff Jens Lykkesfeldt Henning Hvid |
| author_facet | Victoria Svop Jensen Christian Fledelius Erik Max Wulff Jens Lykkesfeldt Henning Hvid |
| author_sort | Victoria Svop Jensen |
| collection | DOAJ |
| description | The use of translationally relevant animal models is essential, also within the field of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Compared to frequently used mouse and rat models, the hamster may provide a higher degree of physiological similarity to humans in terms of lipid profile and lipoprotein metabolism. However, the effects in hamsters after long-term exposure to a NASH diet are not known. Male Syrian hamsters were fed either a high-fat, high-fructose, high-cholesterol diet (NASH diet) or control diets for up to 12 months. Plasma parameters were assessed at two weeks, one, four, eight and 12 months and liver histopathology and biochemistry was characterized after four, eight and 12 months on the experimental diets. After two weeks, hamsters on NASH diet had developed marked dyslipidemia, which persisted for the remainder of the study. Hepatic steatosis was present in NASH-fed hamsters after four months, and hepatic stellate cell activation and fibrosis was observed within four to eight months, respectively, in agreement with progression towards NASH. In summary, we demonstrate that hamsters rapidly develop dyslipidemia when fed a high-fat, high-fructose, high-cholesterol diet. Moreover, within four to eight months, the NASH-diet induced hepatic changes with resemblance to human NAFLD. |
| first_indexed | 2024-03-09T00:56:30Z |
| format | Article |
| id | doaj.art-55c3253c16a24da598fe6ea33b7d5f2f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T00:56:30Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-55c3253c16a24da598fe6ea33b7d5f2f2023-12-11T16:53:21ZengMDPI AGNutrients2072-66432021-02-0113260410.3390/nu13020604Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol DietVictoria Svop Jensen0Christian Fledelius1Erik Max Wulff2Jens Lykkesfeldt3Henning Hvid4Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, DK-1870 Frederiksberg, DenmarkDiabetes Pharmacology 1, Novo Nordisk A/S, Novo Nordisk Park 1, DK-2760 Måløv, DenmarkGubra ApS, Hørsholm Kongevej 11B, DK-2970 Hørsholm, DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, DK-1870 Frederiksberg, DenmarkPathology & Imaging, Novo Nordisk A/S, Novo Nordisk Park 1, DK-2760 Måløv, DenmarkThe use of translationally relevant animal models is essential, also within the field of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Compared to frequently used mouse and rat models, the hamster may provide a higher degree of physiological similarity to humans in terms of lipid profile and lipoprotein metabolism. However, the effects in hamsters after long-term exposure to a NASH diet are not known. Male Syrian hamsters were fed either a high-fat, high-fructose, high-cholesterol diet (NASH diet) or control diets for up to 12 months. Plasma parameters were assessed at two weeks, one, four, eight and 12 months and liver histopathology and biochemistry was characterized after four, eight and 12 months on the experimental diets. After two weeks, hamsters on NASH diet had developed marked dyslipidemia, which persisted for the remainder of the study. Hepatic steatosis was present in NASH-fed hamsters after four months, and hepatic stellate cell activation and fibrosis was observed within four to eight months, respectively, in agreement with progression towards NASH. In summary, we demonstrate that hamsters rapidly develop dyslipidemia when fed a high-fat, high-fructose, high-cholesterol diet. Moreover, within four to eight months, the NASH-diet induced hepatic changes with resemblance to human NAFLD.https://www.mdpi.com/2072-6643/13/2/604dyslipidemianonalcoholic fatty liver diseasenonalcoholic steatohepatitisanimal modelshamster |
| spellingShingle | Victoria Svop Jensen Christian Fledelius Erik Max Wulff Jens Lykkesfeldt Henning Hvid Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet Nutrients dyslipidemia nonalcoholic fatty liver disease nonalcoholic steatohepatitis animal models hamster |
| title | Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet |
| title_full | Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet |
| title_fullStr | Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet |
| title_full_unstemmed | Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet |
| title_short | Temporal Development of Dyslipidemia and Nonalcoholic Fatty Liver Disease (NAFLD) in Syrian Hamsters Fed a High-Fat, High-Fructose, High-Cholesterol Diet |
| title_sort | temporal development of dyslipidemia and nonalcoholic fatty liver disease nafld in syrian hamsters fed a high fat high fructose high cholesterol diet |
| topic | dyslipidemia nonalcoholic fatty liver disease nonalcoholic steatohepatitis animal models hamster |
| url | https://www.mdpi.com/2072-6643/13/2/604 |
| work_keys_str_mv | AT victoriasvopjensen temporaldevelopmentofdyslipidemiaandnonalcoholicfattyliverdiseasenafldinsyrianhamstersfedahighfathighfructosehighcholesteroldiet AT christianfledelius temporaldevelopmentofdyslipidemiaandnonalcoholicfattyliverdiseasenafldinsyrianhamstersfedahighfathighfructosehighcholesteroldiet AT erikmaxwulff temporaldevelopmentofdyslipidemiaandnonalcoholicfattyliverdiseasenafldinsyrianhamstersfedahighfathighfructosehighcholesteroldiet AT jenslykkesfeldt temporaldevelopmentofdyslipidemiaandnonalcoholicfattyliverdiseasenafldinsyrianhamstersfedahighfathighfructosehighcholesteroldiet AT henninghvid temporaldevelopmentofdyslipidemiaandnonalcoholicfattyliverdiseasenafldinsyrianhamstersfedahighfathighfructosehighcholesteroldiet |