Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector
An innovative and sensitive approach using high-performance liquid chromatography-photo diode array detection (HPLC-PDAD) was developed and optimized for the simultaneous determination of abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and three metabolites in eggs. The samples were extracted w...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/23/3894 |
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| author | Yawen Guo Zhaoyuan He Yali Zhu Shuyu Liu Pengfei Gao Kaizhou Xie Tao Zhang Yuhao Dong |
| author_facet | Yawen Guo Zhaoyuan He Yali Zhu Shuyu Liu Pengfei Gao Kaizhou Xie Tao Zhang Yuhao Dong |
| author_sort | Yawen Guo |
| collection | DOAJ |
| description | An innovative and sensitive approach using high-performance liquid chromatography-photo diode array detection (HPLC-PDAD) was developed and optimized for the simultaneous determination of abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and three metabolites in eggs. The samples were extracted with acetonitrile (MeCN)/water (90:10, <i>v</i>/<i>v</i>), and the extracts containing the targets were cleaned up and concentrated by a series of liquid–liquid extraction (LLE) steps. A reversed-phase C18 column and a mobile phase consisting of 0.1% trifluoroacetic acid (TFA) aqueous solution and methanol (MeOH) were utilized to perform optimal chromatographic separation. The developed method was validated on the basis of international guidelines. The limits of detection (LODs) and quantitation (LOQs) were 2.1–10.5 µg/kg and 7.8–28.4 µg/kg, respectively. Satisfactory linear relationships were observed for the targets in their corresponding concentration ranges. The mean recoveries ranged from 85.7% to 97.21% at 4 addition levels, with intraday and interday relative standard deviations (RSDs) in the ranges of 1.68–4.77% and 1.74–5.31%, respectively. The presented protocol was demonstrated to be applicable and reliable by being applied for the detection of target residues in locally sourced egg samples. |
| first_indexed | 2024-03-09T17:47:24Z |
| format | Article |
| id | doaj.art-55c41a5e7d1142e380a5efed0b77ac64 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T17:47:24Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-55c41a5e7d1142e380a5efed0b77ac642023-11-24T11:00:22ZengMDPI AGFoods2304-81582022-12-011123389410.3390/foods11233894Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array DetectorYawen Guo0Zhaoyuan He1Yali Zhu2Shuyu Liu3Pengfei Gao4Kaizhou Xie5Tao Zhang6Yuhao Dong7College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530000, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaAn innovative and sensitive approach using high-performance liquid chromatography-photo diode array detection (HPLC-PDAD) was developed and optimized for the simultaneous determination of abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and three metabolites in eggs. The samples were extracted with acetonitrile (MeCN)/water (90:10, <i>v</i>/<i>v</i>), and the extracts containing the targets were cleaned up and concentrated by a series of liquid–liquid extraction (LLE) steps. A reversed-phase C18 column and a mobile phase consisting of 0.1% trifluoroacetic acid (TFA) aqueous solution and methanol (MeOH) were utilized to perform optimal chromatographic separation. The developed method was validated on the basis of international guidelines. The limits of detection (LODs) and quantitation (LOQs) were 2.1–10.5 µg/kg and 7.8–28.4 µg/kg, respectively. Satisfactory linear relationships were observed for the targets in their corresponding concentration ranges. The mean recoveries ranged from 85.7% to 97.21% at 4 addition levels, with intraday and interday relative standard deviations (RSDs) in the ranges of 1.68–4.77% and 1.74–5.31%, respectively. The presented protocol was demonstrated to be applicable and reliable by being applied for the detection of target residues in locally sourced egg samples.https://www.mdpi.com/2304-8158/11/23/3894abamectinivermectinalbendazoleHPLC–PDADLLE |
| spellingShingle | Yawen Guo Zhaoyuan He Yali Zhu Shuyu Liu Pengfei Gao Kaizhou Xie Tao Zhang Yuhao Dong Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector Foods abamectin ivermectin albendazole HPLC–PDAD LLE |
| title | Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector |
| title_full | Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector |
| title_fullStr | Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector |
| title_full_unstemmed | Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector |
| title_short | Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector |
| title_sort | separation and detection of abamectin ivermectin albendazole and three metabolites in eggs using reversed phase hplc coupled with a photo diode array detector |
| topic | abamectin ivermectin albendazole HPLC–PDAD LLE |
| url | https://www.mdpi.com/2304-8158/11/23/3894 |
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