L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato
InteractionDespite numerous recent insights into neighbor detection and belowground plant communication mediated by root exudates, less is known about the specificity and nature of substances within root exudates and the mechanism by which they may act belowground in root-root interactions.MethodsHe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1056629/full |
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| author | Hongjie Yu Hongjie Yu Hongjie Yu Danmei Gao Danmei Gao Muhammad Khashi u Rahman Muhammad Khashi u Rahman Shaocan Chen Shaocan Chen Fengzhi Wu Fengzhi Wu |
| author_facet | Hongjie Yu Hongjie Yu Hongjie Yu Danmei Gao Danmei Gao Muhammad Khashi u Rahman Muhammad Khashi u Rahman Shaocan Chen Shaocan Chen Fengzhi Wu Fengzhi Wu |
| author_sort | Hongjie Yu |
| collection | DOAJ |
| description | InteractionDespite numerous recent insights into neighbor detection and belowground plant communication mediated by root exudates, less is known about the specificity and nature of substances within root exudates and the mechanism by which they may act belowground in root-root interactions.MethodsHere, we used a coculture experiment to study the root length density (RLD) of tomato (Solanum lycopersicum L.) grown with potato onion (Allium cepa var. aggregatum G. Don) cultivars with growth-promoting (S-potato onion) or no growth-promoting (N-potato onion) effects.Results and DiscussionTomato plants grown with growth-promoting potato onion or its root exudates increased root distribution and length density oppositely and grew their roots away as compared to when grown with potato onion of no growth-promoting potential, its root exudates, and control (tomato monoculture/distilled water treatment). Root exudates profiling of two potato onion cultivars by UPLC-Q-TOF/MS showed that L-phenylalanine was only found in root exudates of S-potato onion. The role of L-phenylalanine was further confirmed in a box experiment in which it altered tomato root distribution and forced the roots grow away. In vitro trial revealed that tomato seedlings root exposed to L-phenylalanine changed the auxin distribution, decreased the concentration of amyloplasts in columella cells of roots, and changed the root deviation angle to grow away from the addition side. These results suggest that L-phenylalanine in S-potato onion root exudates may act as an “active compound” and trigger physio-morphological changes in neighboring tomato roots. |
| first_indexed | 2024-04-10T09:54:06Z |
| format | Article |
| id | doaj.art-55c57a1b2bd64c0d8fdc2a1b56f85b74 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T09:54:06Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-55c57a1b2bd64c0d8fdc2a1b56f85b742023-02-16T12:41:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.10566291056629L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomatoHongjie Yu0Hongjie Yu1Hongjie Yu2Danmei Gao3Danmei Gao4Muhammad Khashi u Rahman5Muhammad Khashi u Rahman6Shaocan Chen7Shaocan Chen8Fengzhi Wu9Fengzhi Wu10Institute of Agricultural Economy and Scientific Information, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Harbin, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Harbin, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Harbin, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Harbin, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Harbin, ChinaInteractionDespite numerous recent insights into neighbor detection and belowground plant communication mediated by root exudates, less is known about the specificity and nature of substances within root exudates and the mechanism by which they may act belowground in root-root interactions.MethodsHere, we used a coculture experiment to study the root length density (RLD) of tomato (Solanum lycopersicum L.) grown with potato onion (Allium cepa var. aggregatum G. Don) cultivars with growth-promoting (S-potato onion) or no growth-promoting (N-potato onion) effects.Results and DiscussionTomato plants grown with growth-promoting potato onion or its root exudates increased root distribution and length density oppositely and grew their roots away as compared to when grown with potato onion of no growth-promoting potential, its root exudates, and control (tomato monoculture/distilled water treatment). Root exudates profiling of two potato onion cultivars by UPLC-Q-TOF/MS showed that L-phenylalanine was only found in root exudates of S-potato onion. The role of L-phenylalanine was further confirmed in a box experiment in which it altered tomato root distribution and forced the roots grow away. In vitro trial revealed that tomato seedlings root exposed to L-phenylalanine changed the auxin distribution, decreased the concentration of amyloplasts in columella cells of roots, and changed the root deviation angle to grow away from the addition side. These results suggest that L-phenylalanine in S-potato onion root exudates may act as an “active compound” and trigger physio-morphological changes in neighboring tomato roots.https://www.frontiersin.org/articles/10.3389/fpls.2023.1056629/fullinterspecific interactionroot exudateschemical cueroot gravitationtomato |
| spellingShingle | Hongjie Yu Hongjie Yu Hongjie Yu Danmei Gao Danmei Gao Muhammad Khashi u Rahman Muhammad Khashi u Rahman Shaocan Chen Shaocan Chen Fengzhi Wu Fengzhi Wu L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato Frontiers in Plant Science interspecific interaction root exudates chemical cue root gravitation tomato |
| title | L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato |
| title_full | L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato |
| title_fullStr | L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato |
| title_full_unstemmed | L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato |
| title_short | L-phenylalanine in potato onion (Allium cepa var. aggregatum G. Don) root exudates mediates neighbor detection and trigger physio-morphological root responses of tomato |
| title_sort | l phenylalanine in potato onion allium cepa var aggregatum g don root exudates mediates neighbor detection and trigger physio morphological root responses of tomato |
| topic | interspecific interaction root exudates chemical cue root gravitation tomato |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1056629/full |
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