Properties of Folate Binding Protein Purified from Cow’s Milk
Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capaci...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2012-09-01
|
| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/view/5621/4265 |
| _version_ | 1818196378417889280 |
|---|---|
| author | SUBANDRATE DWIRINI RETNO GUNARTI MOHAMAD SADIKIN |
| author_facet | SUBANDRATE DWIRINI RETNO GUNARTI MOHAMAD SADIKIN |
| author_sort | SUBANDRATE |
| collection | DOAJ |
| description | Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after -mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control. |
| first_indexed | 2024-12-12T01:33:08Z |
| format | Article |
| id | doaj.art-55c6858739d245e69877efe3e1e5a7be |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-12T01:33:08Z |
| publishDate | 2012-09-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-55c6858739d245e69877efe3e1e5a7be2022-12-22T00:42:55ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942012-09-01193105109Properties of Folate Binding Protein Purified from Cow’s MilkSUBANDRATEDWIRINI RETNO GUNARTIMOHAMAD SADIKINFolic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after -mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.http://journal.ipb.ac.id/index.php/hayati/article/view/5621/4265folate binding proteinfolic acidbinding capacity |
| spellingShingle | SUBANDRATE DWIRINI RETNO GUNARTI MOHAMAD SADIKIN Properties of Folate Binding Protein Purified from Cow’s Milk Hayati Journal of Biosciences folate binding protein folic acid binding capacity |
| title | Properties of Folate Binding Protein Purified from Cow’s Milk |
| title_full | Properties of Folate Binding Protein Purified from Cow’s Milk |
| title_fullStr | Properties of Folate Binding Protein Purified from Cow’s Milk |
| title_full_unstemmed | Properties of Folate Binding Protein Purified from Cow’s Milk |
| title_short | Properties of Folate Binding Protein Purified from Cow’s Milk |
| title_sort | properties of folate binding protein purified from cow s milk |
| topic | folate binding protein folic acid binding capacity |
| url | http://journal.ipb.ac.id/index.php/hayati/article/view/5621/4265 |
| work_keys_str_mv | AT subandrate propertiesoffolatebindingproteinpurifiedfromcowsmilk AT dwiriniretnogunarti propertiesoffolatebindingproteinpurifiedfromcowsmilk AT mohamadsadikin propertiesoffolatebindingproteinpurifiedfromcowsmilk |