Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers
The technique of gently harvesting cells and cell sheets using stimuli-responsive cell culture carriers was applied to human umbilical vein cord endothelial cells (HUVEC). To meet the particular requirements of this demanding cell type, a copolymer consisting of N-isopropylacrylamide and diethyleneg...
| Format: | Article |
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| Language: | English |
| Published: |
Budapest University of Technology
2007-10-01
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| Series: | eXPRESS Polymer Letters |
| Subjects: | |
| Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0000349&mi=cd |
| _version_ | 1819200277143617536 |
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| collection | DOAJ |
| description | The technique of gently harvesting cells and cell sheets using stimuli-responsive cell culture carriers was applied to human umbilical vein cord endothelial cells (HUVEC). To meet the particular requirements of this demanding cell type, a copolymer consisting of N-isopropylacrylamide and diethyleneglycol methacrylate (poly(NiPAAm-co-DEGMA)) was combined with a fine-tuned protein pre-coating. Using this approach the detachment of HUVEC sheets was studied. Furthermore, the behavior of the extracellular matrix upon cell detachment was followed by protein staining. The results demonstrate the feasibility of harvesting HUVEC sheets from stimuli-responsive polymer layers and provide valuable options for the advanced engineering of vascular structures. |
| first_indexed | 2024-12-23T03:29:40Z |
| format | Article |
| id | doaj.art-55c896a62a114959b56786cef2d0f518 |
| institution | Directory Open Access Journal |
| issn | 1788-618X |
| language | English |
| last_indexed | 2024-12-23T03:29:40Z |
| publishDate | 2007-10-01 |
| publisher | Budapest University of Technology |
| record_format | Article |
| series | eXPRESS Polymer Letters |
| spelling | doaj.art-55c896a62a114959b56786cef2d0f5182022-12-21T18:01:43ZengBudapest University of TechnologyeXPRESS Polymer Letters1788-618X2007-10-0111066066610.3144/expresspolymlett.2007.90Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriersThe technique of gently harvesting cells and cell sheets using stimuli-responsive cell culture carriers was applied to human umbilical vein cord endothelial cells (HUVEC). To meet the particular requirements of this demanding cell type, a copolymer consisting of N-isopropylacrylamide and diethyleneglycol methacrylate (poly(NiPAAm-co-DEGMA)) was combined with a fine-tuned protein pre-coating. Using this approach the detachment of HUVEC sheets was studied. Furthermore, the behavior of the extracellular matrix upon cell detachment was followed by protein staining. The results demonstrate the feasibility of harvesting HUVEC sheets from stimuli-responsive polymer layers and provide valuable options for the advanced engineering of vascular structures.http://www.expresspolymlett.com/letolt.php?file=EPL-0000349&mi=cdSmart polymerspolymer gelspoly(N-isopropylacrylamide)tissue engineering |
| spellingShingle | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers eXPRESS Polymer Letters Smart polymers polymer gels poly(N-isopropylacrylamide) tissue engineering |
| title | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers |
| title_full | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers |
| title_fullStr | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers |
| title_full_unstemmed | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers |
| title_short | Detachment of human endothelial cell sheets from thermo-responsive poly(NiPAAm-co-DEGMA) carriers |
| title_sort | detachment of human endothelial cell sheets from thermo responsive poly nipaam co degma carriers |
| topic | Smart polymers polymer gels poly(N-isopropylacrylamide) tissue engineering |
| url | http://www.expresspolymlett.com/letolt.php?file=EPL-0000349&mi=cd |