Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats
Hyperuricemia is the main cause of gout and involved in the occurrence of multiple diseases, such as hypertension, metabolic disorders and chronic kidney disease. Emerging evidence suggests that lactic acid bacteria (LAB) have shown the beneficial effects on the prevention or treatment of hyperurice...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.993951/full |
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| author | Yanjun Li Yanjun Li Yanjun Li Jun Zhu Jun Zhu Guodong Lin Guodong Lin Kan Gao Kan Gao Yunxia Yu Yunxia Yu Su Chen Su Chen Lie Chen Lie Chen Zuoguo Chen Zuoguo Chen Li Li Li Li |
| author_facet | Yanjun Li Yanjun Li Yanjun Li Jun Zhu Jun Zhu Guodong Lin Guodong Lin Kan Gao Kan Gao Yunxia Yu Yunxia Yu Su Chen Su Chen Lie Chen Lie Chen Zuoguo Chen Zuoguo Chen Li Li Li Li |
| author_sort | Yanjun Li |
| collection | DOAJ |
| description | Hyperuricemia is the main cause of gout and involved in the occurrence of multiple diseases, such as hypertension, metabolic disorders and chronic kidney disease. Emerging evidence suggests that lactic acid bacteria (LAB) have shown the beneficial effects on the prevention or treatment of hyperuricemia. In this study, the urate-lowering effect of two LAB strains, Lacticaseibacillus rhamnosus 1155 (LR1155) and Limosilactobacillus fermentum 2644 (LF2644) on hyperuricemic rats were investigated. A hyperuricemic rat model was induced by the intragastric treatment of potassium oxonate, combined with a high purine diet. The oral administration of LR1155, LF2644, or a combination of LR1155 and LF2644 for 4 weeks significantly prevented the rise of the serum uric acid (UA) induced by hyperuricemia. LR1155 and LF2644 significantly elevated the fecal UA levels, increased the UA content and up-regulated gene expression of UA transporter, ATP-binding cassette subfamily G-2 (ABCG2), in colon and jejunum tissues, suggesting the accelerated UA excretion from the intestine. Besides, LR1155 significantly inhibited the activity of xanthine oxidase (XOD) in liver and serum, benefited the reduce of UA production. In addition, LF2644 strengthened the gut barrier functions through an up-regulation of the gene expressions for occluding and mucin2, accompanied with the reduced inflammatory indicators of lipopolysaccharide (LPS) and interleukin-1β (IL-1β) in hyperuricemic rat. Moreover, using 16s rDNA high-throughput sequencing of feces, LR1155 was shown to improve the hyperuricemia induced gut microbial dysbiosis. The genera Roseburia, Butyricicoccus, Prevotella, Oscillibacter, and Bifidobacterium may associate with the effect of LR1155 on microbiota in hyperuricemic rats. Collectively, the results indicated that LR1155 and LF2644 exhibit urate-lowering effects and could be used alone or in combination as a new adjuvant treatment for hyperuricemia. |
| first_indexed | 2024-04-12T03:56:04Z |
| format | Article |
| id | doaj.art-55cd3481548140978dda11f0d8c8437b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T03:56:04Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-55cd3481548140978dda11f0d8c8437b2022-12-22T03:48:50ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.993951993951Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic ratsYanjun Li0Yanjun Li1Yanjun Li2Jun Zhu3Jun Zhu4Guodong Lin5Guodong Lin6Kan Gao7Kan Gao8Yunxia Yu9Yunxia Yu10Su Chen11Su Chen12Lie Chen13Lie Chen14Zuoguo Chen15Zuoguo Chen16Li Li17Li Li18College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaDepartment of Research and Development, Hangzhou Wahaha Group Co., Ltd., Hangzhou, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou, ChinaHyperuricemia is the main cause of gout and involved in the occurrence of multiple diseases, such as hypertension, metabolic disorders and chronic kidney disease. Emerging evidence suggests that lactic acid bacteria (LAB) have shown the beneficial effects on the prevention or treatment of hyperuricemia. In this study, the urate-lowering effect of two LAB strains, Lacticaseibacillus rhamnosus 1155 (LR1155) and Limosilactobacillus fermentum 2644 (LF2644) on hyperuricemic rats were investigated. A hyperuricemic rat model was induced by the intragastric treatment of potassium oxonate, combined with a high purine diet. The oral administration of LR1155, LF2644, or a combination of LR1155 and LF2644 for 4 weeks significantly prevented the rise of the serum uric acid (UA) induced by hyperuricemia. LR1155 and LF2644 significantly elevated the fecal UA levels, increased the UA content and up-regulated gene expression of UA transporter, ATP-binding cassette subfamily G-2 (ABCG2), in colon and jejunum tissues, suggesting the accelerated UA excretion from the intestine. Besides, LR1155 significantly inhibited the activity of xanthine oxidase (XOD) in liver and serum, benefited the reduce of UA production. In addition, LF2644 strengthened the gut barrier functions through an up-regulation of the gene expressions for occluding and mucin2, accompanied with the reduced inflammatory indicators of lipopolysaccharide (LPS) and interleukin-1β (IL-1β) in hyperuricemic rat. Moreover, using 16s rDNA high-throughput sequencing of feces, LR1155 was shown to improve the hyperuricemia induced gut microbial dysbiosis. The genera Roseburia, Butyricicoccus, Prevotella, Oscillibacter, and Bifidobacterium may associate with the effect of LR1155 on microbiota in hyperuricemic rats. Collectively, the results indicated that LR1155 and LF2644 exhibit urate-lowering effects and could be used alone or in combination as a new adjuvant treatment for hyperuricemia.https://www.frontiersin.org/articles/10.3389/fnut.2022.993951/fullhyperuricemialactic acid bacteriaurate-lowering effectintestinal excretionxanthine oxidaseABCG2 |
| spellingShingle | Yanjun Li Yanjun Li Yanjun Li Jun Zhu Jun Zhu Guodong Lin Guodong Lin Kan Gao Kan Gao Yunxia Yu Yunxia Yu Su Chen Su Chen Lie Chen Lie Chen Zuoguo Chen Zuoguo Chen Li Li Li Li Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats Frontiers in Nutrition hyperuricemia lactic acid bacteria urate-lowering effect intestinal excretion xanthine oxidase ABCG2 |
| title | Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats |
| title_full | Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats |
| title_fullStr | Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats |
| title_full_unstemmed | Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats |
| title_short | Probiotic effects of Lacticaseibacillus rhamnosus 1155 and Limosilactobacillus fermentum 2644 on hyperuricemic rats |
| title_sort | probiotic effects of lacticaseibacillus rhamnosus 1155 and limosilactobacillus fermentum 2644 on hyperuricemic rats |
| topic | hyperuricemia lactic acid bacteria urate-lowering effect intestinal excretion xanthine oxidase ABCG2 |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.993951/full |
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