Increased level of B cell differentiation factor in systemic lupus erythematosus patients
Most autoimmune disease are driven by a dysfunction in T and B cells, but B cells are still an interesting area of research, perturbations in their development are implicated in autoimmune diseases. B cell differentiating factor (BCDF) plays a part in the differentiation of B cells. The aim was To a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X1830060X |
| _version_ | 1797205210233307136 |
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| author | Hala Zaki Raslan Hiba Sibaii Salwa Refat El- Zayat Hagar Hassan Mahitab El- Kassaby |
| author_facet | Hala Zaki Raslan Hiba Sibaii Salwa Refat El- Zayat Hagar Hassan Mahitab El- Kassaby |
| author_sort | Hala Zaki Raslan |
| collection | DOAJ |
| description | Most autoimmune disease are driven by a dysfunction in T and B cells, but B cells are still an interesting area of research, perturbations in their development are implicated in autoimmune diseases. B cell differentiating factor (BCDF) plays a part in the differentiation of B cells. The aim was To assess the levels of BCDF, IgM and IgG in SLE patients and whether they have any peculiarity in the clinical context of SLE. Thirty six patients with SLE and 24 healthy volunteers as control were enrolled in the study. BCDF was measured using Sandwich ELISA, total human IgM and IgG were measured by calorimetric methods. The mean concentrations of BCDF and IgM were significantly higher in patients with SLE as compared with controls (P < 0.001 and P < 0.0001 respectively). No significant difference was observed as regard IgG. We observed positive correlation between BCDF and IgM (r = 0.281, P = 0.03), and between IgG and IgM, duration of the disease (r = 0.468, P = 0.004, r = 0.337, P = 0.008 respectively). Moreover we observed lower IgM level in patients with discoid lesion (P = 0.009) and lower IgG level in those with hematologic manifestations (P = 0.02). ROC analysis revealed area under curve (AUC) 0.861 for BCDF and 0.902 for IgM, they can delineate SLE from controls at a cut-off value of 98.5 pg/ml, and 18 mg/dl IgM respectively. Conclusion: BCDF and IgM are increased in SLE patients and are promissing diagnostic markers for SLE. Keywords: BCDF, IgM, IgG, SLE |
| first_indexed | 2024-04-24T08:47:30Z |
| format | Article |
| id | doaj.art-55d0e14fd26d4ff29d4a7c1b82a7634e |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T08:47:30Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-55d0e14fd26d4ff29d4a7c1b82a7634e2024-04-16T13:12:43ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2018-12-01162467471Increased level of B cell differentiation factor in systemic lupus erythematosus patientsHala Zaki Raslan0Hiba Sibaii1Salwa Refat El- Zayat2Hagar Hassan3Mahitab El- Kassaby4Department of Internal Medicine, Medical Division National Research Center, 33 El-Bohouth Street, Dokki, POB:12311, Cairo, EgyptMedical physiology Department Medical Division National Research Center, 33 El-Bohouth Street, Dokki, POB:12311, Cairo, Egypt; Corresponding author.Medical physiology Department Medical Division National Research Center, 33 El-Bohouth Street, Dokki, POB:12311, Cairo, EgyptMedical physiology Department Medical Division National Research Center, 33 El-Bohouth Street, Dokki, POB:12311, Cairo, EgyptMedical physiology Department Medical Division National Research Center, 33 El-Bohouth Street, Dokki, POB:12311, Cairo, EgyptMost autoimmune disease are driven by a dysfunction in T and B cells, but B cells are still an interesting area of research, perturbations in their development are implicated in autoimmune diseases. B cell differentiating factor (BCDF) plays a part in the differentiation of B cells. The aim was To assess the levels of BCDF, IgM and IgG in SLE patients and whether they have any peculiarity in the clinical context of SLE. Thirty six patients with SLE and 24 healthy volunteers as control were enrolled in the study. BCDF was measured using Sandwich ELISA, total human IgM and IgG were measured by calorimetric methods. The mean concentrations of BCDF and IgM were significantly higher in patients with SLE as compared with controls (P < 0.001 and P < 0.0001 respectively). No significant difference was observed as regard IgG. We observed positive correlation between BCDF and IgM (r = 0.281, P = 0.03), and between IgG and IgM, duration of the disease (r = 0.468, P = 0.004, r = 0.337, P = 0.008 respectively). Moreover we observed lower IgM level in patients with discoid lesion (P = 0.009) and lower IgG level in those with hematologic manifestations (P = 0.02). ROC analysis revealed area under curve (AUC) 0.861 for BCDF and 0.902 for IgM, they can delineate SLE from controls at a cut-off value of 98.5 pg/ml, and 18 mg/dl IgM respectively. Conclusion: BCDF and IgM are increased in SLE patients and are promissing diagnostic markers for SLE. Keywords: BCDF, IgM, IgG, SLEhttp://www.sciencedirect.com/science/article/pii/S1687157X1830060X |
| spellingShingle | Hala Zaki Raslan Hiba Sibaii Salwa Refat El- Zayat Hagar Hassan Mahitab El- Kassaby Increased level of B cell differentiation factor in systemic lupus erythematosus patients Journal of Genetic Engineering and Biotechnology |
| title | Increased level of B cell differentiation factor in systemic lupus erythematosus patients |
| title_full | Increased level of B cell differentiation factor in systemic lupus erythematosus patients |
| title_fullStr | Increased level of B cell differentiation factor in systemic lupus erythematosus patients |
| title_full_unstemmed | Increased level of B cell differentiation factor in systemic lupus erythematosus patients |
| title_short | Increased level of B cell differentiation factor in systemic lupus erythematosus patients |
| title_sort | increased level of b cell differentiation factor in systemic lupus erythematosus patients |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X1830060X |
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