Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one opt...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880073/?tool=EBI |
| _version_ | 1818723652598759424 |
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| author | Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter |
| author_facet | Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter |
| author_sort | Oliver Treacy |
| collection | DOAJ |
| description | Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one option to improve their therapeutic potential. Ex-vivo genetic modification of MSCs using recombinant adenovirus (Ad) could be promising to reduce undesired immune responses as Ad will be removed before cell/tissue transplantation. In this regard, we investigated whether Ad-modification of MSCs alters their immunological properties in vitro and in vivo. We found that Ad-transduction of MSCs does not lead to up-regulation of major histocompatibility complex class I and II and co-stimulatory molecules CD80 and CD86. Moreover, Ad-transduction caused no significant changes in terms of pro-inflammatory cytokine expression, chemokine and chemokine receptor and Toll-like receptor expression. In addition, Ad-modification of MSCs had no affect on their ability to suppress T cell proliferation in vitro. In vivo injection of Ad-transduced MSCs did not change the frequency of various immune cell populations (antigen presenting cells, T helper and cytotoxic T cells, natural killer and natural killer T cells) neither in the blood nor in tissues. Our results indicate that Ad-modification has no major influence on the immunological properties of MSCs and therefore can be considered as a suitable gene vector for therapeutic applications of MSCs. |
| first_indexed | 2024-12-17T21:13:56Z |
| format | Article |
| id | doaj.art-55d431c8d85e47059b111fe8ebca5251 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T21:13:56Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-55d431c8d85e47059b111fe8ebca52512022-12-21T21:32:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4266210.1371/journal.pone.0042662Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.Oliver TreacyAideen E RyanTeresa HeinzlLisa O'FlynnMarese CreggMieszko WilkFrancesca OdoardiPaul LohanTimothy O'BrienMikhail NosovThomas RitterAdult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one option to improve their therapeutic potential. Ex-vivo genetic modification of MSCs using recombinant adenovirus (Ad) could be promising to reduce undesired immune responses as Ad will be removed before cell/tissue transplantation. In this regard, we investigated whether Ad-modification of MSCs alters their immunological properties in vitro and in vivo. We found that Ad-transduction of MSCs does not lead to up-regulation of major histocompatibility complex class I and II and co-stimulatory molecules CD80 and CD86. Moreover, Ad-transduction caused no significant changes in terms of pro-inflammatory cytokine expression, chemokine and chemokine receptor and Toll-like receptor expression. In addition, Ad-modification of MSCs had no affect on their ability to suppress T cell proliferation in vitro. In vivo injection of Ad-transduced MSCs did not change the frequency of various immune cell populations (antigen presenting cells, T helper and cytotoxic T cells, natural killer and natural killer T cells) neither in the blood nor in tissues. Our results indicate that Ad-modification has no major influence on the immunological properties of MSCs and therefore can be considered as a suitable gene vector for therapeutic applications of MSCs.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880073/?tool=EBI |
| spellingShingle | Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. PLoS ONE |
| title | Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. |
| title_full | Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. |
| title_fullStr | Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. |
| title_full_unstemmed | Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. |
| title_short | Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. |
| title_sort | adenoviral transduction of mesenchymal stem cells in vitro responses and in vivo immune responses after cell transplantation |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880073/?tool=EBI |
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