Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture
Bartonella species represent important causative agents of blood culture-negative infective endocarditis (IE). Their diagnosis represents a challenge for microbiologists and often relies on serological and molecular tools. However, even if the sensitivity of blood culture remains low, it should not...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | IDCases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221425092200275X |
| _version_ | 1811178986786521088 |
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| author | M. Robert R. Lecomte M. Michel G. Guimbretiere G. Croizier S. Corvec A.G. Leroy |
| author_facet | M. Robert R. Lecomte M. Michel G. Guimbretiere G. Croizier S. Corvec A.G. Leroy |
| author_sort | M. Robert |
| collection | DOAJ |
| description | Bartonella species represent important causative agents of blood culture-negative infective endocarditis (IE). Their diagnosis represents a challenge for microbiologists and often relies on serological and molecular tools. However, even if the sensitivity of blood culture remains low, it should not be definitely ruled out. Indeed, we report the unusual case of a 22 year-old Guinean homeless man diagnosed with an IE due to Bartonella quintana. Unexpectedly, conventional blood cultures were positive after 13 days of incubation. Subculture was obtained on blood and chocolate agar, after 15 days of incubation in a 5 % CO2 atmosphere. Bacterial identification was obtained up to the species level using molecular tools (16S rRNA gene amplification and sequencing). A literature review of B. quintana blood culture-positive IE was conducted and revealed eighteen similar reported cases on a 25-year period. This case illustrates that, despite low sensitivity, Bartonella IE may be diagnosed thanks to prolonged blood culture. |
| first_indexed | 2024-04-11T06:28:14Z |
| format | Article |
| id | doaj.art-55d48f53d4134825b32a9519d1c5fe9a |
| institution | Directory Open Access Journal |
| issn | 2214-2509 |
| language | English |
| last_indexed | 2024-04-11T06:28:14Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IDCases |
| spelling | doaj.art-55d48f53d4134825b32a9519d1c5fe9a2022-12-22T04:40:17ZengElsevierIDCases2214-25092022-01-0130e01647Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood cultureM. Robert0R. Lecomte1M. Michel2G. Guimbretiere3G. Croizier4S. Corvec5A.G. Leroy6Université de Nantes, CHU Nantes, Service de Bactériologie et des Contrôles Microbiologiques, F-44000 Nantes, France; Correspondence to: Service de Bactériologie et des Contrôles Microbiologiques, 9 quai Moncousu, Centre Hospitalier Universitaire de Nantes, 44 093 NANTES Cedex 1, France.Nantes Université, CHU Nantes, INSERM, Service de Maladies infectieuses et Tropicales, CIC 1413, F-44000 Nantes, FranceDepartment of Cardiology, CHU Nantes, Nantes, FranceDepartment of Thoracic and Cardiovascular Surgery, CHU Nantes, Nantes, FranceUniversité de Nantes, CHU Nantes, Service de Bactériologie et des Contrôles Microbiologiques, F-44000 Nantes, FranceUniversité de Nantes, CHU Nantes, Service de Bactériologie et des Contrôles Microbiologiques, F-44000 Nantes, France; Université de Nantes, CHU Nantes, INSERM, INCIT UMR 1302, F-44000 Nantes, FranceLaboratoire de Microbiologie, CHU Sud Réunion, Saint-Pierre, La Réunion, FranceBartonella species represent important causative agents of blood culture-negative infective endocarditis (IE). Their diagnosis represents a challenge for microbiologists and often relies on serological and molecular tools. However, even if the sensitivity of blood culture remains low, it should not be definitely ruled out. Indeed, we report the unusual case of a 22 year-old Guinean homeless man diagnosed with an IE due to Bartonella quintana. Unexpectedly, conventional blood cultures were positive after 13 days of incubation. Subculture was obtained on blood and chocolate agar, after 15 days of incubation in a 5 % CO2 atmosphere. Bacterial identification was obtained up to the species level using molecular tools (16S rRNA gene amplification and sequencing). A literature review of B. quintana blood culture-positive IE was conducted and revealed eighteen similar reported cases on a 25-year period. This case illustrates that, despite low sensitivity, Bartonella IE may be diagnosed thanks to prolonged blood culture.http://www.sciencedirect.com/science/article/pii/S221425092200275XBartonella quintanaBlood culture-positiveInfective endocarditisMolecular tools |
| spellingShingle | M. Robert R. Lecomte M. Michel G. Guimbretiere G. Croizier S. Corvec A.G. Leroy Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture IDCases Bartonella quintana Blood culture-positive Infective endocarditis Molecular tools |
| title | Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| title_full | Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| title_fullStr | Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| title_full_unstemmed | Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| title_short | Bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| title_sort | bartonella quintana infective endocarditis in a homeless man with unexpected positive blood culture |
| topic | Bartonella quintana Blood culture-positive Infective endocarditis Molecular tools |
| url | http://www.sciencedirect.com/science/article/pii/S221425092200275X |
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