Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System
The strawberry (<i>Fragaria × ananassa</i> Duch.) is an important fruit crop cultivated worldwide for its unique taste and nutritional properties. One of the major risks associated with strawberry production is cold damage. Recently, melatonin has emerged as a multifunctional signaling m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/3/194 |
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| author | Faisal Hayat Zixun Sun Zhaojun Ni Shahid Iqbal Wuyi Xu Zhihong Gao Yushan Qiao Muhammad Aammar Tufail Mohammad Shah Jahan Ummara Khan Chunyan Wan Xianbin Gu |
| author_facet | Faisal Hayat Zixun Sun Zhaojun Ni Shahid Iqbal Wuyi Xu Zhihong Gao Yushan Qiao Muhammad Aammar Tufail Mohammad Shah Jahan Ummara Khan Chunyan Wan Xianbin Gu |
| author_sort | Faisal Hayat |
| collection | DOAJ |
| description | The strawberry (<i>Fragaria × ananassa</i> Duch.) is an important fruit crop cultivated worldwide for its unique taste and nutritional properties. One of the major risks associated with strawberry production is cold damage. Recently, melatonin has emerged as a multifunctional signaling molecule that influences plant growth and development and reduces adverse consequences of cold stress. The present study was conducted to investigate the defensive role of melatonin and its potential interrelation with abscisic acid (ABA) in strawberry plants under cold stress. The results demonstrate that melatonin application conferred improved cold tolerance on strawberry seedlings by reducing malondialdehyde and hydrogen peroxide contents under cold stress. Conversely, pretreatment of strawberry plants with 100 μM melatonin increased soluble sugar contents and different antioxidant enzyme activities (ascorbate peroxidase, catalase, and peroxidase) and non-enzymatic antioxidant (ascorbate and glutathione) activities under cold stress. Furthermore, exogenous melatonin treatment stimulated the expression of the DREB/CBF—COR pathways’ downstream genes. Interestingly, ABA treatment did not change the expression of the DREB/CBF—COR pathway. These findings imply that the DREB/CBF-COR pathway confers cold tolerance on strawberry seedlings through exogenous melatonin application. Taken together, our results reveal that melatonin (100 μM) pretreatment protects strawberry plants from the damages induced by cold stress through enhanced antioxidant defense potential and modulating the DREB/CBF—COR pathway. |
| first_indexed | 2024-03-09T19:45:19Z |
| format | Article |
| id | doaj.art-55d749432f3749c3b9d976f159b42471 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T19:45:19Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-55d749432f3749c3b9d976f159b424712023-11-24T01:25:05ZengMDPI AGHorticulturae2311-75242022-02-018319410.3390/horticulturae8030194Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense SystemFaisal Hayat0Zixun Sun1Zhaojun Ni2Shahid Iqbal3Wuyi Xu4Zhihong Gao5Yushan Qiao6Muhammad Aammar Tufail7Mohammad Shah Jahan8Ummara Khan9Chunyan Wan10Xianbin Gu11Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute for General Microbiology, Christian-Albrechts-Universität zu Kiel Am Botanischen Garten 1-9, 24118 Kiel, GermanyDepartment of Horticulture, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshKey Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaZhenjiang Agricultural Science Research Institute, Jurong 212400, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe strawberry (<i>Fragaria × ananassa</i> Duch.) is an important fruit crop cultivated worldwide for its unique taste and nutritional properties. One of the major risks associated with strawberry production is cold damage. Recently, melatonin has emerged as a multifunctional signaling molecule that influences plant growth and development and reduces adverse consequences of cold stress. The present study was conducted to investigate the defensive role of melatonin and its potential interrelation with abscisic acid (ABA) in strawberry plants under cold stress. The results demonstrate that melatonin application conferred improved cold tolerance on strawberry seedlings by reducing malondialdehyde and hydrogen peroxide contents under cold stress. Conversely, pretreatment of strawberry plants with 100 μM melatonin increased soluble sugar contents and different antioxidant enzyme activities (ascorbate peroxidase, catalase, and peroxidase) and non-enzymatic antioxidant (ascorbate and glutathione) activities under cold stress. Furthermore, exogenous melatonin treatment stimulated the expression of the DREB/CBF—COR pathways’ downstream genes. Interestingly, ABA treatment did not change the expression of the DREB/CBF—COR pathway. These findings imply that the DREB/CBF-COR pathway confers cold tolerance on strawberry seedlings through exogenous melatonin application. Taken together, our results reveal that melatonin (100 μM) pretreatment protects strawberry plants from the damages induced by cold stress through enhanced antioxidant defense potential and modulating the DREB/CBF—COR pathway.https://www.mdpi.com/2311-7524/8/3/194melatoninabiotic stresscold toleranceantioxidant potentialgene expression |
| spellingShingle | Faisal Hayat Zixun Sun Zhaojun Ni Shahid Iqbal Wuyi Xu Zhihong Gao Yushan Qiao Muhammad Aammar Tufail Mohammad Shah Jahan Ummara Khan Chunyan Wan Xianbin Gu Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System Horticulturae melatonin abiotic stress cold tolerance antioxidant potential gene expression |
| title | Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System |
| title_full | Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System |
| title_fullStr | Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System |
| title_full_unstemmed | Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System |
| title_short | Exogenous Melatonin Improves Cold Tolerance of Strawberry (<i>Fragaria × ananassa</i> Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System |
| title_sort | exogenous melatonin improves cold tolerance of strawberry i fragaria ananassa i duch through modulation of dreb cbf cor pathway and antioxidant defense system |
| topic | melatonin abiotic stress cold tolerance antioxidant potential gene expression |
| url | https://www.mdpi.com/2311-7524/8/3/194 |
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