Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neu...
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Format: | Article |
Language: | English |
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eLife Sciences Publications Ltd
2014-10-01
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Series: | eLife |
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Online Access: | https://elifesciences.org/articles/03726 |
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author | Timothy J Mosca Liqun Luo |
author_facet | Timothy J Mosca Liqun Luo |
author_sort | Timothy J Mosca |
collection | DOAJ |
description | Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms. |
first_indexed | 2024-12-10T05:01:39Z |
format | Article |
id | doaj.art-55d8d0f36bd34726b27e4f83c97edc61 |
institution | Directory Open Access Journal |
issn | 2050-084X |
language | English |
last_indexed | 2024-12-10T05:01:39Z |
publishDate | 2014-10-01 |
publisher | eLife Sciences Publications Ltd |
record_format | Article |
series | eLife |
spelling | doaj.art-55d8d0f36bd34726b27e4f83c97edc612022-12-22T02:01:22ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03726Synaptic organization of the Drosophila antennal lobe and its regulation by the TeneurinsTimothy J Mosca0https://orcid.org/0000-0003-3485-0719Liqun Luo1Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesUnderstanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms.https://elifesciences.org/articles/03726synapseolfactionTeneurinantennal lobedrosophila |
spellingShingle | Timothy J Mosca Liqun Luo Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins eLife synapse olfaction Teneurin antennal lobe drosophila |
title | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_full | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_fullStr | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_full_unstemmed | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_short | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_sort | synaptic organization of the drosophila antennal lobe and its regulation by the teneurins |
topic | synapse olfaction Teneurin antennal lobe drosophila |
url | https://elifesciences.org/articles/03726 |
work_keys_str_mv | AT timothyjmosca synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins AT liqunluo synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins |