Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins

Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neu...

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Main Authors: Timothy J Mosca, Liqun Luo
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-10-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/03726
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author Timothy J Mosca
Liqun Luo
author_facet Timothy J Mosca
Liqun Luo
author_sort Timothy J Mosca
collection DOAJ
description Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms.
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spelling doaj.art-55d8d0f36bd34726b27e4f83c97edc612022-12-22T02:01:22ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03726Synaptic organization of the Drosophila antennal lobe and its regulation by the TeneurinsTimothy J Mosca0https://orcid.org/0000-0003-3485-0719Liqun Luo1Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesUnderstanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms.https://elifesciences.org/articles/03726synapseolfactionTeneurinantennal lobedrosophila
spellingShingle Timothy J Mosca
Liqun Luo
Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
eLife
synapse
olfaction
Teneurin
antennal lobe
drosophila
title Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
title_full Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
title_fullStr Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
title_full_unstemmed Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
title_short Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
title_sort synaptic organization of the drosophila antennal lobe and its regulation by the teneurins
topic synapse
olfaction
Teneurin
antennal lobe
drosophila
url https://elifesciences.org/articles/03726
work_keys_str_mv AT timothyjmosca synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins
AT liqunluo synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins