Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes
Utilization of R (resistance) genes to develop resistant cultivars is an effective strategy to combat against rice blast disease. In this study, R genes Pi46 and Pita in a resistant accession H4 were introgressed into an elite restorer line Hang-Hui-179 (HH179) using the marker-assisted backcross br...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916614156 |
| _version_ | 1818839004960784384 |
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| author | Wu-ming XIAO Li-xin LUO Hui WANG Tao GUO Yong-zhu LIU Ji-yong ZHOU Xiao-yuan ZHU Qi-yun YANG Zhi-qiang CHEN |
| author_facet | Wu-ming XIAO Li-xin LUO Hui WANG Tao GUO Yong-zhu LIU Ji-yong ZHOU Xiao-yuan ZHU Qi-yun YANG Zhi-qiang CHEN |
| author_sort | Wu-ming XIAO |
| collection | DOAJ |
| description | Utilization of R (resistance) genes to develop resistant cultivars is an effective strategy to combat against rice blast disease. In this study, R genes Pi46 and Pita in a resistant accession H4 were introgressed into an elite restorer line Hang-Hui-179 (HH179) using the marker-assisted backcross breeding (MABB) procedure. As a result, three improved lines (e.g., R1791 carrying Pi46 alone, R1792 carrying Pita alone and R1793 carrying both Pi46 and Pita) were developed. The three improved lines had significant genetic similarities with the recurrent parent HH179. Thus, they and HH179 could be recognized as near isogenic lines (NILs). The resistance spectrum of the three improved lines, which was tested at seedling stage, reached 91.1, 64.7 and 97.1%, respectively. This was markedly broader than that of HH179 (23.5%). Interestingly, R1793 showed resistance to panicle blast but neither R1791 nor R1792 exhibited resistance at two natural blast nurseries. The results implied that the stacking of Pi46 and Pita resulted in enhanced resistance, which was unachievable by either R gene alone. Further comparison indicated that the three improved lines were similar to HH179 in multiple agronomic traits; including plant height, tillers per plant, panicle length, spikelet fertility, and 1 000-grain weight. Thus, the three improved lines with different R genes can be used as new sources of resistance for developing variety. There is a complementary effect between the two R genes Pi46 and Pita. |
| first_indexed | 2024-12-19T03:47:24Z |
| format | Article |
| id | doaj.art-55daa7de5e2443568d6c06b5b4884083 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T03:47:24Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-55daa7de5e2443568d6c06b5b48840832022-12-21T20:37:05ZengElsevierJournal of Integrative Agriculture2095-31192016-10-01151022902298Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genesWu-ming XIAO0Li-xin LUO1Hui WANG2Tao GUO3Yong-zhu LIU4Ji-yong ZHOU5Xiao-yuan ZHU6Qi-yun YANG7Zhi-qiang CHEN8National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. China; XIAO Wu-mingNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. ChinaPlant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R. ChinaPlant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R. China; Correspondence YANG Qi-yunNational Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R. China; CHEN Zhi-qiangUtilization of R (resistance) genes to develop resistant cultivars is an effective strategy to combat against rice blast disease. In this study, R genes Pi46 and Pita in a resistant accession H4 were introgressed into an elite restorer line Hang-Hui-179 (HH179) using the marker-assisted backcross breeding (MABB) procedure. As a result, three improved lines (e.g., R1791 carrying Pi46 alone, R1792 carrying Pita alone and R1793 carrying both Pi46 and Pita) were developed. The three improved lines had significant genetic similarities with the recurrent parent HH179. Thus, they and HH179 could be recognized as near isogenic lines (NILs). The resistance spectrum of the three improved lines, which was tested at seedling stage, reached 91.1, 64.7 and 97.1%, respectively. This was markedly broader than that of HH179 (23.5%). Interestingly, R1793 showed resistance to panicle blast but neither R1791 nor R1792 exhibited resistance at two natural blast nurseries. The results implied that the stacking of Pi46 and Pita resulted in enhanced resistance, which was unachievable by either R gene alone. Further comparison indicated that the three improved lines were similar to HH179 in multiple agronomic traits; including plant height, tillers per plant, panicle length, spikelet fertility, and 1 000-grain weight. Thus, the three improved lines with different R genes can be used as new sources of resistance for developing variety. There is a complementary effect between the two R genes Pi46 and Pita.http://www.sciencedirect.com/science/article/pii/S2095311916614156rice blastresistance geneimprovementmarker-assisted selection |
| spellingShingle | Wu-ming XIAO Li-xin LUO Hui WANG Tao GUO Yong-zhu LIU Ji-yong ZHOU Xiao-yuan ZHU Qi-yun YANG Zhi-qiang CHEN Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes Journal of Integrative Agriculture rice blast resistance gene improvement marker-assisted selection |
| title | Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes |
| title_full | Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes |
| title_fullStr | Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes |
| title_full_unstemmed | Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes |
| title_short | Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes |
| title_sort | pyramiding of pi46 and pita to improve blast resistance and to evaluate the resistance effect of the two r genes |
| topic | rice blast resistance gene improvement marker-assisted selection |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916614156 |
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