miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster
Circadian clocks temporally organize behaviour and physiology of organisms with a rhythmicity of about 24 h. In Drosophila, the circadian clock is composed of mainly four clock genes: period (per), timeless (tim), Clock (Clk) and cycle (cyc) which constitutes the transcription-translation feedback l...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1082866/full |
| _version_ | 1797449280118587392 |
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| author | Geo Anna Maria John Nisha N. Kannan |
| author_facet | Geo Anna Maria John Nisha N. Kannan |
| author_sort | Geo Anna |
| collection | DOAJ |
| description | Circadian clocks temporally organize behaviour and physiology of organisms with a rhythmicity of about 24 h. In Drosophila, the circadian clock is composed of mainly four clock genes: period (per), timeless (tim), Clock (Clk) and cycle (cyc) which constitutes the transcription-translation feedback loop. The circadian clock is further regulated via post-transcriptional and post-translational mechanisms among which microRNAs (miRNAs) are well known post-transcriptional regulatory molecules. Here, we identified and characterized the role of miRNA-277 (miR-277) expressed in the clock neurons in regulating the circadian rhythm. Downregulation of miR-277 in the pacemaker neurons expressing circadian neuropeptide, pigment dispersing factor (PDF) advanced the phase of the morning activity peak under 12 h light: 12 h dark cycles (LD) at lower light intensities and these flies exhibited less robust rhythms compared to the controls under constant darkness. In addition, downregulation of miR-277 in the PDF expressing neurons abolished the Clk gene transcript oscillation under LD. Our study points to the potential role of miR-277 in fine tuning the Clk expression and in maintaining the phase of the circadian rhythm in Drosophila. |
| first_indexed | 2024-03-09T14:22:39Z |
| format | Article |
| id | doaj.art-55dd357b8500484e8cc9c4fbdaa81d78 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-09T14:22:39Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-55dd357b8500484e8cc9c4fbdaa81d782023-11-28T09:27:15ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-11-011410.3389/fphys.2023.10828661082866miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogasterGeo AnnaMaria JohnNisha N. KannanCircadian clocks temporally organize behaviour and physiology of organisms with a rhythmicity of about 24 h. In Drosophila, the circadian clock is composed of mainly four clock genes: period (per), timeless (tim), Clock (Clk) and cycle (cyc) which constitutes the transcription-translation feedback loop. The circadian clock is further regulated via post-transcriptional and post-translational mechanisms among which microRNAs (miRNAs) are well known post-transcriptional regulatory molecules. Here, we identified and characterized the role of miRNA-277 (miR-277) expressed in the clock neurons in regulating the circadian rhythm. Downregulation of miR-277 in the pacemaker neurons expressing circadian neuropeptide, pigment dispersing factor (PDF) advanced the phase of the morning activity peak under 12 h light: 12 h dark cycles (LD) at lower light intensities and these flies exhibited less robust rhythms compared to the controls under constant darkness. In addition, downregulation of miR-277 in the PDF expressing neurons abolished the Clk gene transcript oscillation under LD. Our study points to the potential role of miR-277 in fine tuning the Clk expression and in maintaining the phase of the circadian rhythm in Drosophila.https://www.frontiersin.org/articles/10.3389/fphys.2023.1082866/fullcircadianphaseamplitudefree running periodmiR-277light intensity |
| spellingShingle | Geo Anna Maria John Nisha N. Kannan miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster Frontiers in Physiology circadian phase amplitude free running period miR-277 light intensity |
| title | miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster |
| title_full | miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster |
| title_fullStr | miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster |
| title_full_unstemmed | miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster |
| title_short | miR-277 regulates the phase of circadian activity-rest rhythm in Drosophila melanogaster |
| title_sort | mir 277 regulates the phase of circadian activity rest rhythm in drosophila melanogaster |
| topic | circadian phase amplitude free running period miR-277 light intensity |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1082866/full |
| work_keys_str_mv | AT geoanna mir277regulatesthephaseofcircadianactivityrestrhythmindrosophilamelanogaster AT mariajohn mir277regulatesthephaseofcircadianactivityrestrhythmindrosophilamelanogaster AT nishankannan mir277regulatesthephaseofcircadianactivityrestrhythmindrosophilamelanogaster |