Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma
ABSTRACT Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regard...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100082&lng=en&tlng=en |
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| author | M.B.R. Faleiro L.C. Cintra R.S.A. Jesuino A.D. Damasceno V.M.B.D. Moura |
| author_facet | M.B.R. Faleiro L.C. Cintra R.S.A. Jesuino A.D. Damasceno V.M.B.D. Moura |
| author_sort | M.B.R. Faleiro |
| collection | DOAJ |
| description | ABSTRACT Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regarding number of p27 and p53 labeled cells, difference between normal and PIA and PC was observed, as well as between PIA and PC for p53. Immunostaining intensities of p21, p27 and p53 were different when comparing normal tissues to PIA and PC. Sixteen cDNA of canine prostatic FFPE tissue were subjected to RT-PCR and RT-qPCR, four normal, three PIA, and nine PC. CDKN1A mRNA was detected in four PC by RT-PCR, and it was overexpressed when compared to normal by RT-qPCR, in one PIA and six PC. CDKN1B mRNA was detected in three PC by RT-PCR and it was overexpressed in three PC and decreased in one PC. TP53 mRNA was overexpressed in one PIA and three PC. In conclusion, when overexpressed in canine prostate with premalignant and malignant, p21 and p27 play a role controlling cell proliferation, working as a protective factor in the evolution of PIA to PC, and in the PC development, even in the presence of altered p53. |
| first_indexed | 2024-04-12T21:51:50Z |
| format | Article |
| id | doaj.art-55ddc9a786d94afb8d246b9e50725324 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-04-12T21:51:50Z |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-55ddc9a786d94afb8d246b9e507253242022-12-22T03:15:28ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-4162701829210.1590/1678-4162-9472S0102-09352018000100082Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinomaM.B.R. FaleiroL.C. CintraR.S.A. JesuinoA.D. DamascenoV.M.B.D. MouraABSTRACT Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regarding number of p27 and p53 labeled cells, difference between normal and PIA and PC was observed, as well as between PIA and PC for p53. Immunostaining intensities of p21, p27 and p53 were different when comparing normal tissues to PIA and PC. Sixteen cDNA of canine prostatic FFPE tissue were subjected to RT-PCR and RT-qPCR, four normal, three PIA, and nine PC. CDKN1A mRNA was detected in four PC by RT-PCR, and it was overexpressed when compared to normal by RT-qPCR, in one PIA and six PC. CDKN1B mRNA was detected in three PC by RT-PCR and it was overexpressed in three PC and decreased in one PC. TP53 mRNA was overexpressed in one PIA and three PC. In conclusion, when overexpressed in canine prostate with premalignant and malignant, p21 and p27 play a role controlling cell proliferation, working as a protective factor in the evolution of PIA to PC, and in the PC development, even in the presence of altered p53.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100082&lng=en&tlng=enp21p27p53PIART-qPCR |
| spellingShingle | M.B.R. Faleiro L.C. Cintra R.S.A. Jesuino A.D. Damasceno V.M.B.D. Moura Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma Arquivo Brasileiro de Medicina Veterinária e Zootecnia p21 p27 p53 PIA RT-qPCR |
| title | Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| title_full | Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| title_fullStr | Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| title_full_unstemmed | Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| title_short | Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| title_sort | expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma |
| topic | p21 p27 p53 PIA RT-qPCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100082&lng=en&tlng=en |
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