An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis
Feline infectious peritonitis (FIP) is a systemic disease manifestation of feline coronavirus (FCoV) and is the most important cause of infectious disease-related deaths in domestic cats. FIP has a variable clinical manifestation but is most often characterized by widespread vasculitis with visceral...
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MDPI AG
2024-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/1/141 |
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| author | Benjamin E. Curtis Zaid Abdo Barbara Graham Alora LaVoy Samantha J. M. Evans Kelly Santangelo Gregg A. Dean |
| author_facet | Benjamin E. Curtis Zaid Abdo Barbara Graham Alora LaVoy Samantha J. M. Evans Kelly Santangelo Gregg A. Dean |
| author_sort | Benjamin E. Curtis |
| collection | DOAJ |
| description | Feline infectious peritonitis (FIP) is a systemic disease manifestation of feline coronavirus (FCoV) and is the most important cause of infectious disease-related deaths in domestic cats. FIP has a variable clinical manifestation but is most often characterized by widespread vasculitis with visceral involvement and/or neurological disease that is typically fatal in the absence of antiviral therapy. Using an aptamer-based proteomics assay, we analyzed the plasma protein profiles of cats who were naturally infected with FIP (n = 19) in comparison to the plasma protein profiles of cats who were clinically healthy and negative for FCoV (n = 17) and cats who were positive for the enteric form of FCoV (n = 9). We identified 442 proteins that were significantly differentiable; in total, 219 increased and 223 decreased in FIP plasma versus clinically healthy cat plasma. Pathway enrichment and associated analyses showed that differentiable proteins were related to immune system processes, including the innate immune response, cytokine signaling, and antigen presentation, as well as apoptosis and vascular integrity. The relevance of these findings is discussed in the context of previous studies. While these results have the potential to inform diagnostic, therapeutic, and preventative investigations, they represent only a first step, and will require further validation. |
| first_indexed | 2024-03-08T10:32:25Z |
| format | Article |
| id | doaj.art-55dfa71375b44bc598209a4e2c0b1acc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T10:32:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-55dfa71375b44bc598209a4e2c0b1acc2024-01-26T18:48:34ZengMDPI AGViruses1999-49152024-01-0116114110.3390/v16010141An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious PeritonitisBenjamin E. Curtis0Zaid Abdo1Barbara Graham2Alora LaVoy3Samantha J. M. Evans4Kelly Santangelo5Gregg A. Dean6Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USAFeline infectious peritonitis (FIP) is a systemic disease manifestation of feline coronavirus (FCoV) and is the most important cause of infectious disease-related deaths in domestic cats. FIP has a variable clinical manifestation but is most often characterized by widespread vasculitis with visceral involvement and/or neurological disease that is typically fatal in the absence of antiviral therapy. Using an aptamer-based proteomics assay, we analyzed the plasma protein profiles of cats who were naturally infected with FIP (n = 19) in comparison to the plasma protein profiles of cats who were clinically healthy and negative for FCoV (n = 17) and cats who were positive for the enteric form of FCoV (n = 9). We identified 442 proteins that were significantly differentiable; in total, 219 increased and 223 decreased in FIP plasma versus clinically healthy cat plasma. Pathway enrichment and associated analyses showed that differentiable proteins were related to immune system processes, including the innate immune response, cytokine signaling, and antigen presentation, as well as apoptosis and vascular integrity. The relevance of these findings is discussed in the context of previous studies. While these results have the potential to inform diagnostic, therapeutic, and preventative investigations, they represent only a first step, and will require further validation.https://www.mdpi.com/1999-4915/16/1/141feline infectious peritonitisFIPprotein profileproteomicspathway analysisenrichment |
| spellingShingle | Benjamin E. Curtis Zaid Abdo Barbara Graham Alora LaVoy Samantha J. M. Evans Kelly Santangelo Gregg A. Dean An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis Viruses feline infectious peritonitis FIP protein profile proteomics pathway analysis enrichment |
| title | An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis |
| title_full | An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis |
| title_fullStr | An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis |
| title_full_unstemmed | An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis |
| title_short | An Aptamer-Based Proteomic Analysis of Plasma from Cats (<i>Felis catus</i>) with Clinical Feline Infectious Peritonitis |
| title_sort | aptamer based proteomic analysis of plasma from cats i felis catus i with clinical feline infectious peritonitis |
| topic | feline infectious peritonitis FIP protein profile proteomics pathway analysis enrichment |
| url | https://www.mdpi.com/1999-4915/16/1/141 |
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