Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1
Penta-<i>O</i>-galloyl-β-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a gallotannin polyphenolic compound that occurs naturally in fermented <i>Rhus verniciflua</i>. The present study aimed to examine the effect of PGG on UV...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/11/561 |
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| author | Ji-An Kim Jae-Eun Lee Ji Hye Kim Hyo-Jeong Lee Nam Joo Kang |
| author_facet | Ji-An Kim Jae-Eun Lee Ji Hye Kim Hyo-Jeong Lee Nam Joo Kang |
| author_sort | Ji-An Kim |
| collection | DOAJ |
| description | Penta-<i>O</i>-galloyl-β-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a gallotannin polyphenolic compound that occurs naturally in fermented <i>Rhus verniciflua</i>. The present study aimed to examine the effect of PGG on UVB-induced skin aging and its molecular mechanisms in HaCaT human keratinocytes and SKH-1 hairless mice models. PGG suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in HaCaT cells by inhibiting phosphorylation of RAF/MEK/ERK, MKK3/6/p38, and c-Jun. UVB-induced ERK and p38 signaling pathways that induce the MMP-1 expression were mediated by PAK1 in HaCaT cells. PGG suppressed PAK1 and JNK1 kinase activities, and directly bound both PAK1 in an ATP-competitive manner and JNK1 in an ATP-noncompetitive manner. Consistently, PGG decreased UVB-induced wrinkle formation, epidermal thickness, type 1 collagen and MMP-13 expression in mouse skin. Overall, these results indicate that PGG exhibits anti-photoaging effects in vitro and in vivo by the suppression of PAK1 and JNK1 kinase activities, and may be useful for the prevention of skin aging. |
| first_indexed | 2024-03-12T10:36:32Z |
| format | Article |
| id | doaj.art-55dfffa2328441f4a8ada8dd4a99d36a |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T10:36:32Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-55dfffa2328441f4a8ada8dd4a99d36a2023-09-02T08:37:11ZengMDPI AGAntioxidants2076-39212019-11-0181156110.3390/antiox8110561antiox8110561Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1Ji-An Kim0Jae-Eun Lee1Ji Hye Kim2Hyo-Jeong Lee3Nam Joo Kang4School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaCollege of Korean Medicine, Cancer Preventive Material Research Center, Kyunghee University, Seoul 02447, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaPenta-<i>O</i>-galloyl-β-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a gallotannin polyphenolic compound that occurs naturally in fermented <i>Rhus verniciflua</i>. The present study aimed to examine the effect of PGG on UVB-induced skin aging and its molecular mechanisms in HaCaT human keratinocytes and SKH-1 hairless mice models. PGG suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in HaCaT cells by inhibiting phosphorylation of RAF/MEK/ERK, MKK3/6/p38, and c-Jun. UVB-induced ERK and p38 signaling pathways that induce the MMP-1 expression were mediated by PAK1 in HaCaT cells. PGG suppressed PAK1 and JNK1 kinase activities, and directly bound both PAK1 in an ATP-competitive manner and JNK1 in an ATP-noncompetitive manner. Consistently, PGG decreased UVB-induced wrinkle formation, epidermal thickness, type 1 collagen and MMP-13 expression in mouse skin. Overall, these results indicate that PGG exhibits anti-photoaging effects in vitro and in vivo by the suppression of PAK1 and JNK1 kinase activities, and may be useful for the prevention of skin aging.https://www.mdpi.com/2076-3921/8/11/561penta-1,2,3,4,6-<i>o</i>-galloyl-β-<span style="font-variant: small-caps">d</span>-glucosejnk1pak1photoagingultraviolet b |
| spellingShingle | Ji-An Kim Jae-Eun Lee Ji Hye Kim Hyo-Jeong Lee Nam Joo Kang Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 Antioxidants penta-1,2,3,4,6-<i>o</i>-galloyl-β-<span style="font-variant: small-caps">d</span>-glucose jnk1 pak1 photoaging ultraviolet b |
| title | Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 |
| title_full | Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 |
| title_fullStr | Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 |
| title_full_unstemmed | Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 |
| title_short | Penta-1,2,3,4,6-<i>O</i>-Galloyl-β-<span style="font-variant: small-caps">d</span>-Glucose Inhibits UVB-Induced Photoaging by Targeting PAK1 and JNK1 |
| title_sort | penta 1 2 3 4 6 i o i galloyl β span style font variant small caps d span glucose inhibits uvb induced photoaging by targeting pak1 and jnk1 |
| topic | penta-1,2,3,4,6-<i>o</i>-galloyl-β-<span style="font-variant: small-caps">d</span>-glucose jnk1 pak1 photoaging ultraviolet b |
| url | https://www.mdpi.com/2076-3921/8/11/561 |
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