Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program
Clam farmers worldwide face several challenges, including irregular seed supply and high mortalities due to pathogenic organisms such as Perkinsus olseni. In Europe, there is a high unmet consumer demand for native clam species such as Ruditapes decussatus. The high market value of R. decussatus mak...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/9728.pdf |
| _version_ | 1797419291397586944 |
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| author | Andreia Cruz Fiz da Costa Jenyfer Fernández-Pérez Ana Nantón Sergio Fernández-Boo Ana Insua Josefina Méndez |
| author_facet | Andreia Cruz Fiz da Costa Jenyfer Fernández-Pérez Ana Nantón Sergio Fernández-Boo Ana Insua Josefina Méndez |
| author_sort | Andreia Cruz |
| collection | DOAJ |
| description | Clam farmers worldwide face several challenges, including irregular seed supply and high mortalities due to pathogenic organisms such as Perkinsus olseni. In Europe, there is a high unmet consumer demand for native clam species such as Ruditapes decussatus. The high market value of R. decussatus makes the culture of this species potentially more attractive than that culture of the alien species Ruditapes philippinarum. Thus, there is a market opportunity in breeding and producing R. decussatus at an industrial scale. A selective breeding program to improve R. decussatus performance will be carried out in Portugal; and the first critical step to develop such a breeding program is the establishment of a founder population. In this study, intra- and interpopulation genetic diversity was assessed using 13 microsatellite markers in eight natural beds located in Portugal, Spain and Italy. Also, allele and genotypic frequencies of each microsatellite locus were assessed discriminating between clams infected and non-infected by P. olseni. All locations showed similar values for several genetic diversity parameters. Analyses of population differentiation (FST, Bayesian clustering and AMOVAs) revealed five genetically differentiated regions: Rías Altas and Rías Baixas (NW Spain), North/Central Coast of Portugal, Gulf of Cadiz and Adriatic Sea. Significant differences in the allelic and genotypic frequency distribution between infected clams and non-infected ones at four microsatellite loci are reported suggesting that resistance to the disease could have a genetic basis. Moreover, a positive or negative relationship between the frequency of certain alleles and the parasite infection was inferred. Further studies should confirm the potential use of those alleles as genetic markers for P. olseni infection. Integrating results of genetic diversity within and between populations and Perkinsus infection levels, a founder population for a R. decussatus breeding program is proposed, composed by individuals from Barallobre (Rías Altas), Pontevedra or Cangas (Rías Baixas), Óbidos (North/Central Coast of Portugal), Algarve (Gulf of Cadiz) and Venice (Adriatic Sea). |
| first_indexed | 2024-03-09T06:46:16Z |
| format | Article |
| id | doaj.art-55e0dd86f444434cb4cc91ad3b8a7fe3 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:46:16Z |
| publishDate | 2020-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-55e0dd86f444434cb4cc91ad3b8a7fe32023-12-03T10:35:22ZengPeerJ Inc.PeerJ2167-83592020-10-018e972810.7717/peerj.9728Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding programAndreia Cruz0Fiz da Costa1Jenyfer Fernández-Pérez2Ana Nantón3Sergio Fernández-Boo4Ana Insua5Josefina Méndez6Oceano Fresco S.A., Nazaré, PortugalOceano Fresco S.A., Nazaré, PortugalGrupo Xenomar, Departamento de Biología, Facultade de Ciencias and Centro de Investigación Científicas Avanzadas (CICA), Universidad de La Coruña, A Coruña, SpainGrupo Xenomar, Departamento de Biología, Facultade de Ciencias and Centro de Investigación Científicas Avanzadas (CICA), Universidad de La Coruña, A Coruña, SpainCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Matosinhos, PortugalGrupo Xenomar, Departamento de Biología, Facultade de Ciencias and Centro de Investigación Científicas Avanzadas (CICA), Universidad de La Coruña, A Coruña, SpainGrupo Xenomar, Departamento de Biología, Facultade de Ciencias and Centro de Investigación Científicas Avanzadas (CICA), Universidad de La Coruña, A Coruña, SpainClam farmers worldwide face several challenges, including irregular seed supply and high mortalities due to pathogenic organisms such as Perkinsus olseni. In Europe, there is a high unmet consumer demand for native clam species such as Ruditapes decussatus. The high market value of R. decussatus makes the culture of this species potentially more attractive than that culture of the alien species Ruditapes philippinarum. Thus, there is a market opportunity in breeding and producing R. decussatus at an industrial scale. A selective breeding program to improve R. decussatus performance will be carried out in Portugal; and the first critical step to develop such a breeding program is the establishment of a founder population. In this study, intra- and interpopulation genetic diversity was assessed using 13 microsatellite markers in eight natural beds located in Portugal, Spain and Italy. Also, allele and genotypic frequencies of each microsatellite locus were assessed discriminating between clams infected and non-infected by P. olseni. All locations showed similar values for several genetic diversity parameters. Analyses of population differentiation (FST, Bayesian clustering and AMOVAs) revealed five genetically differentiated regions: Rías Altas and Rías Baixas (NW Spain), North/Central Coast of Portugal, Gulf of Cadiz and Adriatic Sea. Significant differences in the allelic and genotypic frequency distribution between infected clams and non-infected ones at four microsatellite loci are reported suggesting that resistance to the disease could have a genetic basis. Moreover, a positive or negative relationship between the frequency of certain alleles and the parasite infection was inferred. Further studies should confirm the potential use of those alleles as genetic markers for P. olseni infection. Integrating results of genetic diversity within and between populations and Perkinsus infection levels, a founder population for a R. decussatus breeding program is proposed, composed by individuals from Barallobre (Rías Altas), Pontevedra or Cangas (Rías Baixas), Óbidos (North/Central Coast of Portugal), Algarve (Gulf of Cadiz) and Venice (Adriatic Sea).https://peerj.com/articles/9728.pdfRuditapes decussatusSelective breedingPerkinsus infectionGenetic diversityMicrosatellites |
| spellingShingle | Andreia Cruz Fiz da Costa Jenyfer Fernández-Pérez Ana Nantón Sergio Fernández-Boo Ana Insua Josefina Méndez Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program PeerJ Ruditapes decussatus Selective breeding Perkinsus infection Genetic diversity Microsatellites |
| title | Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program |
| title_full | Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program |
| title_fullStr | Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program |
| title_full_unstemmed | Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program |
| title_short | Genetic variability in Ruditapes decussatus clam combined with Perkinsus infection level to support founder population selection for a breeding program |
| title_sort | genetic variability in ruditapes decussatus clam combined with perkinsus infection level to support founder population selection for a breeding program |
| topic | Ruditapes decussatus Selective breeding Perkinsus infection Genetic diversity Microsatellites |
| url | https://peerj.com/articles/9728.pdf |
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