Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis
Shixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and MarkerlynxTM analysis....
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| Format: | Article |
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MDPI AG
2010-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/15/9/6217/ |
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| author | Er-Xin Shang Da-Wei Qian Jian-Ming Guo Jin-Ao Duan Shu-Lan Su Wei Zhou Jin Zhang |
| author_facet | Er-Xin Shang Da-Wei Qian Jian-Ming Guo Jin-Ao Duan Shu-Lan Su Wei Zhou Jin Zhang |
| author_sort | Er-Xin Shang |
| collection | DOAJ |
| description | Shixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and MarkerlynxTM analysis. The analgesic activities of two extracts of Shixiao San were evaluated using the hot-plate test, acetic acid-induced writhing and dysmenorrhea mice model. The results showed that the analgesic activity of Shixiao San vinegary extract (boiling vinegar) was superior to the aqueous extract. UPLC-QTOF and MarkerlynxTM analytic results showed that the process of boiling in vinegar may improve the dissolution of Shixiao San flavanoids, and these flavanoids may contribute to the observed analgesic activity. This work demonstrated that UPLC/QTOFMS and MarkerlynxTM could serve as new methods for fast generation and automated analysis of information-rich data from Chinese herbal medicines. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T06:03:39Z |
| publishDate | 2010-09-01 |
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| spelling | doaj.art-55e25fc14ffd4035b47f9bd8278a8edf2022-12-21T21:18:36ZengMDPI AGMolecules1420-30492010-09-011596217623010.3390/molecules15096217Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx AnalysisEr-Xin ShangDa-Wei QianJian-Ming GuoJin-Ao DuanShu-Lan SuWei ZhouJin ZhangShixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and MarkerlynxTM analysis. The analgesic activities of two extracts of Shixiao San were evaluated using the hot-plate test, acetic acid-induced writhing and dysmenorrhea mice model. The results showed that the analgesic activity of Shixiao San vinegary extract (boiling vinegar) was superior to the aqueous extract. UPLC-QTOF and MarkerlynxTM analytic results showed that the process of boiling in vinegar may improve the dissolution of Shixiao San flavanoids, and these flavanoids may contribute to the observed analgesic activity. This work demonstrated that UPLC/QTOFMS and MarkerlynxTM could serve as new methods for fast generation and automated analysis of information-rich data from Chinese herbal medicines.http://www.mdpi.com/1420-3049/15/9/6217/Shixiao Sananalgesic activityactive componentsUPLC-QTOF-MSMarkerlynxTM |
| spellingShingle | Er-Xin Shang Da-Wei Qian Jian-Ming Guo Jin-Ao Duan Shu-Lan Su Wei Zhou Jin Zhang Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis Molecules Shixiao San analgesic activity active components UPLC-QTOF-MS MarkerlynxTM |
| title | Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis |
| title_full | Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis |
| title_fullStr | Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis |
| title_full_unstemmed | Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis |
| title_short | Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis |
| title_sort | characterization of the active constituents in shixiao san using bioactivity evaluation followed by uplc qtof and markerlynx analysis |
| topic | Shixiao San analgesic activity active components UPLC-QTOF-MS MarkerlynxTM |
| url | http://www.mdpi.com/1420-3049/15/9/6217/ |
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