Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein
Erythrocyte recognition and invasion is critical for the intra-erythrocytic development of Plasmodium spp. parasites. The multistep invasion process involves specific interactions between parasite ligands and erythrocyte receptors. Erythrocyte-binding-like (EBL) proteins, type I integral transmembra...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-04-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.656620/full |
| _version_ | 1818878738879741952 |
|---|---|
| author | Takaaki Yuguchi Bernard N. Kanoi Hikaru Nagaoka Toyokazu Miura Daisuke Ito Hiroyuki Takeda Takafumi Tsuboi Eizo Takashima Hitoshi Otsuki |
| author_facet | Takaaki Yuguchi Bernard N. Kanoi Hikaru Nagaoka Toyokazu Miura Daisuke Ito Hiroyuki Takeda Takafumi Tsuboi Eizo Takashima Hitoshi Otsuki |
| author_sort | Takaaki Yuguchi |
| collection | DOAJ |
| description | Erythrocyte recognition and invasion is critical for the intra-erythrocytic development of Plasmodium spp. parasites. The multistep invasion process involves specific interactions between parasite ligands and erythrocyte receptors. Erythrocyte-binding-like (EBL) proteins, type I integral transmembrane proteins released from the merozoite micronemes, are known to play an important role in the initiation and formation of tight junctions between the apical end of the merozoite and the erythrocyte surface. In Plasmodium yoelii EBL (PyEBL), a single amino acid substitution in the putative Duffy binding domain dramatically changes parasite growth rate and virulence. This suggests that PyEBL is important for modulating the virulence of P. yoelii parasites. Based on these observations, we sought to elucidate the receptor of PyEBL that mediates its role as an invasion ligand. Using the eukaryotic wheat germ cell-free system, we systematically developed and screened a library of mouse erythrocyte proteins against native PyEBL using AlphaScreen technology. We report that PyEBL specifically interacts with basigin, an erythrocyte surface protein. We further confirmed that the N-terminal cysteine-rich Duffy binding-like region (EBL region 2), is responsible for the interaction, and that the binding is not affected by the C351Y mutation, which was previously shown to modulate virulence of P. yoelii. The identification of basigin as the putative PyEBL receptor offers new insights into the role of this molecule and provides an important base for in-depth studies towards developing novel interventions against malaria. |
| first_indexed | 2024-12-19T14:18:57Z |
| format | Article |
| id | doaj.art-55e7d9587c3e43b7a66dda713f5b5fea |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-19T14:18:57Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-55e7d9587c3e43b7a66dda713f5b5fea2022-12-21T20:17:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-04-011110.3389/fcimb.2021.656620656620Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface ProteinTakaaki Yuguchi0Bernard N. Kanoi1Hikaru Nagaoka2Toyokazu Miura3Daisuke Ito4Hiroyuki Takeda5Takafumi Tsuboi6Eizo Takashima7Hitoshi Otsuki8Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Medical Zoology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, JapanDivision of Proteo-Drug-Discovery Sciences, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, JapanDivision of Medical Zoology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, JapanErythrocyte recognition and invasion is critical for the intra-erythrocytic development of Plasmodium spp. parasites. The multistep invasion process involves specific interactions between parasite ligands and erythrocyte receptors. Erythrocyte-binding-like (EBL) proteins, type I integral transmembrane proteins released from the merozoite micronemes, are known to play an important role in the initiation and formation of tight junctions between the apical end of the merozoite and the erythrocyte surface. In Plasmodium yoelii EBL (PyEBL), a single amino acid substitution in the putative Duffy binding domain dramatically changes parasite growth rate and virulence. This suggests that PyEBL is important for modulating the virulence of P. yoelii parasites. Based on these observations, we sought to elucidate the receptor of PyEBL that mediates its role as an invasion ligand. Using the eukaryotic wheat germ cell-free system, we systematically developed and screened a library of mouse erythrocyte proteins against native PyEBL using AlphaScreen technology. We report that PyEBL specifically interacts with basigin, an erythrocyte surface protein. We further confirmed that the N-terminal cysteine-rich Duffy binding-like region (EBL region 2), is responsible for the interaction, and that the binding is not affected by the C351Y mutation, which was previously shown to modulate virulence of P. yoelii. The identification of basigin as the putative PyEBL receptor offers new insights into the role of this molecule and provides an important base for in-depth studies towards developing novel interventions against malaria.https://www.frontiersin.org/articles/10.3389/fcimb.2021.656620/fullPlasmodium yoeliiPyEBLbasigininvasionprotein-protein interactionCD147 |
| spellingShingle | Takaaki Yuguchi Bernard N. Kanoi Hikaru Nagaoka Toyokazu Miura Daisuke Ito Hiroyuki Takeda Takafumi Tsuboi Eizo Takashima Hitoshi Otsuki Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein Frontiers in Cellular and Infection Microbiology Plasmodium yoelii PyEBL basigin invasion protein-protein interaction CD147 |
| title | Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein |
| title_full | Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein |
| title_fullStr | Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein |
| title_full_unstemmed | Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein |
| title_short | Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein |
| title_sort | plasmodium yoelii erythrocyte binding like protein interacts with basigin an erythrocyte surface protein |
| topic | Plasmodium yoelii PyEBL basigin invasion protein-protein interaction CD147 |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.656620/full |
| work_keys_str_mv | AT takaakiyuguchi plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT bernardnkanoi plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT hikarunagaoka plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT toyokazumiura plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT daisukeito plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT hiroyukitakeda plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT takafumitsuboi plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT eizotakashima plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein AT hitoshiotsuki plasmodiumyoeliierythrocytebindinglikeproteininteractswithbasiginanerythrocytesurfaceprotein |