Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications
Poor osteogenesis and bacterial infections lead to an implant failure, so the enhanced osteogenic and antimicrobial activity of the implantable device is of great importance in orthopedic applications. In this study, 2-methacryloyloxyethyl phosphocholine (MPC) was grafted onto 316L stainless steel (...
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MDPI AG
2019-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/7/939 |
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| author | Dave W. Chen Hsin-Hsin Yu Li-Jyuan Luo Selvaraj Rajesh Kumar Chien-Hao Chen Tung-Yi Lin Jui-Yang Lai Shingjiang Jessie Lue |
| author_facet | Dave W. Chen Hsin-Hsin Yu Li-Jyuan Luo Selvaraj Rajesh Kumar Chien-Hao Chen Tung-Yi Lin Jui-Yang Lai Shingjiang Jessie Lue |
| author_sort | Dave W. Chen |
| collection | DOAJ |
| description | Poor osteogenesis and bacterial infections lead to an implant failure, so the enhanced osteogenic and antimicrobial activity of the implantable device is of great importance in orthopedic applications. In this study, 2-methacryloyloxyethyl phosphocholine (MPC) was grafted onto 316L stainless steel (SS) using a facile photo-induced radical graft polymerization method via a benzophenone (BP) photo initiator. Atomic force microscopy (AFM) was employed to determine the nanoscale morphological changes on the surface. The grafted BP-MPC layer was estimated to be tens of nanometers thick. The SS-BP-MPC composite was more hydrophilic and smoother than the untreated and BP-treated SS samples. <i>Staphylococcus aureus</i> (<i>S. aureus</i>) bacteria binding onto the SS-BP-MPC composite film surface was significantly reduced compared with the pristine SS and SS-BP samples. Mouse pre-osteoblast (MC3T3-E1) cells showed good adhesion on the MPC-modified samples and better proliferation and metabolic activity (73% higher) than the pristine SS sample. Biological studies revealed that grafting MPC onto the SS substrate enhanced the antibacterial efficiency and also retained osteoblast biocompatibility. This proposed procedure is promising for use with other implant materials. |
| first_indexed | 2024-12-14T22:38:53Z |
| format | Article |
| id | doaj.art-55e83d1039454399acbb384e202aa569 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-14T22:38:53Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-55e83d1039454399acbb384e202aa5692022-12-21T22:45:02ZengMDPI AGNanomaterials2079-49912019-06-019793910.3390/nano9070939nano9070939Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant ApplicationsDave W. Chen0Hsin-Hsin Yu1Li-Jyuan Luo2Selvaraj Rajesh Kumar3Chien-Hao Chen4Tung-Yi Lin5Jui-Yang Lai6Shingjiang Jessie Lue7Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung City 401, TaiwanDepartment of Chemical and Materials Engineering and Green Technology Research Center, Chang Gung University, Guishan District, Taoyuan City 333, TaiwanGraduate institute of Biomedical Engineering, Chang Gung University, Guishan District, Taoyuan City 333, TaiwanDepartment of Chemical and Materials Engineering and Green Technology Research Center, Chang Gung University, Guishan District, Taoyuan City 333, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung City 401, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung City 401, TaiwanGraduate institute of Biomedical Engineering, Chang Gung University, Guishan District, Taoyuan City 333, TaiwanDepartment of Chemical and Materials Engineering and Green Technology Research Center, Chang Gung University, Guishan District, Taoyuan City 333, TaiwanPoor osteogenesis and bacterial infections lead to an implant failure, so the enhanced osteogenic and antimicrobial activity of the implantable device is of great importance in orthopedic applications. In this study, 2-methacryloyloxyethyl phosphocholine (MPC) was grafted onto 316L stainless steel (SS) using a facile photo-induced radical graft polymerization method via a benzophenone (BP) photo initiator. Atomic force microscopy (AFM) was employed to determine the nanoscale morphological changes on the surface. The grafted BP-MPC layer was estimated to be tens of nanometers thick. The SS-BP-MPC composite was more hydrophilic and smoother than the untreated and BP-treated SS samples. <i>Staphylococcus aureus</i> (<i>S. aureus</i>) bacteria binding onto the SS-BP-MPC composite film surface was significantly reduced compared with the pristine SS and SS-BP samples. Mouse pre-osteoblast (MC3T3-E1) cells showed good adhesion on the MPC-modified samples and better proliferation and metabolic activity (73% higher) than the pristine SS sample. Biological studies revealed that grafting MPC onto the SS substrate enhanced the antibacterial efficiency and also retained osteoblast biocompatibility. This proposed procedure is promising for use with other implant materials.https://www.mdpi.com/2079-4991/9/7/9392-methacryloyloxyethyl phosphocholine (MPC)antibacterial activityosseointegrationphoto-induced polymerizationorthopedic implants |
| spellingShingle | Dave W. Chen Hsin-Hsin Yu Li-Jyuan Luo Selvaraj Rajesh Kumar Chien-Hao Chen Tung-Yi Lin Jui-Yang Lai Shingjiang Jessie Lue Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications Nanomaterials 2-methacryloyloxyethyl phosphocholine (MPC) antibacterial activity osseointegration photo-induced polymerization orthopedic implants |
| title | Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications |
| title_full | Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications |
| title_fullStr | Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications |
| title_full_unstemmed | Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications |
| title_short | Osteoblast Biocompatibility and Antibacterial Effects Using 2-Methacryloyloxyethyl Phosphocholine-Grafted Stainless-Steel Composite for Implant Applications |
| title_sort | osteoblast biocompatibility and antibacterial effects using 2 methacryloyloxyethyl phosphocholine grafted stainless steel composite for implant applications |
| topic | 2-methacryloyloxyethyl phosphocholine (MPC) antibacterial activity osseointegration photo-induced polymerization orthopedic implants |
| url | https://www.mdpi.com/2079-4991/9/7/939 |
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